Efficient coupled transcription/ translation from PCR template by a hollow-fiber membrane bioreactor

Biotechnology and Bioengineering

Volumn 64, Issue 2, 1999, Pages 194-199

  Nakano, Hideo ^a   Shinbata, Tomoya ^a   Okumura, Reiko ^a   Sekiguchi, Satoshi ^b   Fujishiro, Masatoshi ^c   Yamane, Tsuneo ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b Kanagawa 243 0041   (Japan)

^c Tomy Seiko Co Ltd   (Japan)

Author keywords

Bioreactor; Coupled transcription translation; Hollow fiber membrane; Polymerase chain reaction; T7 terminator

Indexed keywords

BIOLOGICAL MEMBRANES; BIOREACTORS; BIOSYNTHESIS; ENZYMES; ESCHERICHIA COLI; FIBROUS MEMBRANES; MASS TRANSFER; SUBSTRATES;

HOLLOW-FIBER MEMBRANES; POLYMERASE CHAIN REACTION (PCR);

GENETIC ENGINEERING;

GENE PRODUCT; MESSENGER RNA; NUCLEOTIDE; PROTEIN;

ARTICLE; CONCENTRATION (PARAMETERS); DNA FLANKING REGION; DNA TEMPLATE; ESCHERICHIA COLI; GENETIC TRANSCRIPTION; HOLLOW FIBER MEMBRANE; MEMBRANE REACTOR; MESSENGER RNA SYNTHESIS; POLYMERASE CHAIN REACTION; PROTEIN SYNTHESIS; REGULATOR GENE; REPORTER GENE; RNA TRANSLATION;

BASE SEQUENCE; BIOREACTORS; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PROTEIN BIOSYNTHESIS; TIME FACTORS; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

ESCHERICHIA; ESCHERICHIA COLI; UNIDENTIFIED BACTERIOPHAGE;

EID: 0033587797     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990720)64:2<194::AID-BIT8>3.0.CO;2-5     Document Type: Article

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