Deoxycholate induces DNA damage and apoptosis in human colon epithelial cells expressing either mutant or wild-type p53

International Journal of Biochemistry and Cell Biology

Volumn 33, Issue 2, 2001, Pages 193-203

  Powolny, Anna ^a,b   Xu, Jing ^a   Loo, George ^a  

^a UNIVERSITY OF NORTH CAROLINA AT GREENSBORO   (United States)

^b UNIVERSITY OF WROC AW   (Poland)

Author keywords

Apoptosis; Bile acids; Deoxycholate; DNA damage; p53

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; BILE ACID; CELL DNA; DEOXYCHOLIC ACID; DEOXYURIDINE TRIPHOSPHATE PYROPHOSPHATASE; DNA FRAGMENT; FLUORESCEIN ISOTHIOCYANATE; LIPOCORTIN 5; MUTANT PROTEIN; PROTEIN P53;

ANALYTIC METHOD; APOPTOSIS; ARTICLE; CELL LINE; CHROMATIN CONDENSATION; COLON INJURY; COLON MUCOSA; CONTROLLED STUDY; DNA DAMAGE; DNA STRAND BREAKAGE; EPITHELIUM CELL; GENE EXPRESSION; GENOTOXICITY; HUMAN; HUMAN CELL; PROTEIN BINDING; TUMOR SUPPRESSOR GENE;

ADENOCARCINOMA; APOPTOSIS; COLONIC NEOPLASMS; COMET ASSAY; DEOXYCHOLIC ACID; DNA DAMAGE; EPITHELIAL CELLS; GENES, P53; HUMANS; IN SITU NICK-END LABELING; MICROSCOPY, FLUORESCENCE; MUTATION; STAINING AND LABELING; TUMOR CELLS, CULTURED;

EID: 0035251866     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1357-2725(00)00080-7     Document Type: Article

References (44)

1. Lessons from hereditary colorectal cancer
2. Dietary factors in human colorectal cancer
3. Nutritional factors and colon cancer
4. The role of fat, fatty acids, and total energy intake in the etiology of human colon cancer
5. Faecal steroids and colorectal carcinogenesis
6. The continuing importance of bile acids in liver and intestinal disease
7. Effect of dietary deoxycholic acid and cholesterol on fecal steroid concentration and its impact on the colonic crypt cell proliferation in azoxymethane-treated rats
8. Enhancing effect of bile and bile acid on stomach tumorigenesis induced by N′-methyl-N′-nitro-N-nitrosoguanidine in Wistar rats
9. Faecal unconjugated bile acids in patients with colorectal cancer or polyps
10. In vitro formation of DNA adducts with bile acids
11. Bile acids, non-phorbol-ester-type tumor promoters, stimulate the phosphorylation of protein kinase C substrates in human platelets and colon cell line HT29
12. Induction of the transcription factor AP-1 in cultured human colon adenocarcinoma cells following exposure to bile acids
13. The stress-response proteins poly(ADP-ribose)polymerase and NF-κB protect against bile salt-induced apoptosis
14. Dihydroxy bile acids activate the transcription of cyclooxygenase-2
15. Expression of cyclooxygenase-1 and -2 in human colorectal cancer
16. Secondary bile acid induced DNA damage in HT29 cells: Are free radicals involved
17. Free radicals and antioxidants: A personal view
18. Life and death by p53
19. Role of apoptosis in biology and pathology: Resistance to apoptosis in colon carcinogenesis
20. Structure and function of the p53 tumor suppressor gene: Clues for rational cancer therapeutic strategies
21. DNA-dependent protein kinase inhibitor (OK-1035) suppresses p21 expression in HCT116 cells containing wild-type p53 induced by adriamycin
22. Antioxidants enhance the cytotoxicity of chemotherapeutic agents in colorectal cancer: A p53-independent induction of p21WAF1/CIP1 via C/EBP
23. A simple technique for quantitation of low levels of DNA damage in individual cells
24. Effect of copper deficiency on oxidative DNA damage in Jurkat T-lymphocytes
25. Effects of epigallocatechin gallate and quercetin on oxidative damage to cellular DNA
26. Evaluation of manual and image analysis quantitation of DNA damage in the alkaline comet assay
27. Effect of high fat consumption on cell proliferation activity of colorectal mucosa and on soluble faecal bile acids
28. Genotoxic activity in human faecal water and the role of bole acids: A case study using the alkaline comet assay
29. Generation of hydroperoxides in isolated rat hepatocytes and hepatic mitochondria exposed to hydrophobic bile acids
30. Evidence for involvement of oxygen free radicals in bile acid toxicity to isolated rat hepatocytes
31. Reactions of oxyl radicals with DNA
32. Electrochemical detection of 8-hydroxy-2-deoxyguanosine levels in cellular DNA
33. Role of nitric oxide and peroxynitrite in bile salt-induced apoptosis: Relevance to colon carcinogenesis
34. The chemistry of DNA damage from nitric oxide and peroxynitrite
35. Possible mechanisms involved in apoptosis of colon tumor cell lines induced by deoxycholic acid, short-chain fatty acids, and their mixtures
36. Effect of colonic luminal components on induction of apoptosis in human colonic cell lines
37. Mechanisms of p53-induced apoptosis
38. Induction of the WAF1/CIP1 protein and apoptosis in human T-cell leukemia virus type I-transformed lymphocytes after treatment with adriamycin by using a p53-independent pathway
39. Bile acid activation of the gadd 153 promoter and of p53-independent apoptosis: Relevance to colon cancer
40. Wild-type p53 protein potentiates phototoxicity of 2-BA-2-DMBA in HT29 cells expressing endogenous mutant p53
41. Enterocyte differentiation is compatible with SV40 large T expression and loss of p53 function in human colonic Caco-2 cells. Status of the pRb1 and pRb2 tumor suppressor gene products
42. Toxic bile acids induce rodent hepatocyte apoptosis via direct activation of Fas
43. The fas signaling pathway is functional in colon carcinoma cells and induces apoptosis