Evaluation of manual and image analysis quantification of DNA damage in the alkaline comet assay

Mutagenesis

Volumn 12, Issue 4, 1997, Pages 209-214

  McCarthy, P J ^a   Sweetman, S F ^a   McKenna, P G ^a   McKelvey Martin, V J ^a  

^a UNIVERSITY OF ULSTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; HYDROGEN PEROXIDE;

ANIMAL CELL; ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; DNA DAMAGE; DNA STRAND BREAKAGE; ERYTHROLEUKEMIA CELL; GEL ELECTROPHORESIS; IMAGE ANALYSIS; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; DNA; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, AGAR GEL; HYDROGEN PEROXIDE; IMAGE PROCESSING, COMPUTER-ASSISTED; LEUKEMIA, ERYTHROBLASTIC, ACUTE; MICE; MUTAGENICITY TESTS; OXIDANTS; REPRODUCIBILITY OF RESULTS; TUMOR CELLS, CULTURED;

ANIMALIA;

EID: 0030758092     PISSN: 02678357     EISSN: None     Source Type: Journal    
DOI: 10.1093/mutage/12.4.209     Document Type: Article

References (10)

1. Dizdaroglu,M., Rao,G., Halliwell,B. and Gajewski,E. (1991) Damage to the DNA bases in mammalian chromatin by hydrogen peroxide in the presence of ferric and cupric ions. Arch. Biochem. Biophys., 285, 317-324.
2. Gedik,A.M., Ewen,S.W.B. and Collins,A.R. (1992) Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells. Int. J. Radiat. Biol., 62, 313-320.
3. McKelvey,V.J., Mckerr,G., Joyner,D. and Brown,M. (1992a) The DNA comet as an indicator of DNA damage. Nature (UK) Product Review August,p. 30.
4. McKelvey,V.J., McKerr,G. and Joyner,D. (1992b) The comet assay and DNA damage. Microsc. Anal., 29,9.
5. McKelvey-Martin,V.J., Green,M., Schmezer,P., Pool-Zobel,B.L., DeMeo,M.P. and Collins,A. (1993) The single cell gel electrophoresis assay (comet assay): a European review. Mutat. Res., 288, 47-63.
6. McKelvey,V.J., Tice,R.R. and Singh,N.P. (1990) A technique for measuring DNA single strand breaks and alkali labile sites in individual cells. Br. J. Cancer, 62, 529.
7. Olive,P.L. and Ranath,J.P. (1993) Induction and rejoining of radiation induced DNA single strand breaks: Tail moment as a function of position in the cell cycle. Mutat. Res. (DNA repair), 294, 275-283.
8. Olive,P.L., Wlodek,R.E., Durand,R.E. and Banath,J.P. (1992) Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp. Cell Res., 198, 259-267.
9. Olive,P.L., Banath,J.P. and Durand,R.P. (1990) Heterogeneity in radiation induced DNA damage and repair in tumour and normal cells measured during the 'comet' assay. Radiat. Res., 122, 86-94.
10. Singh,N.P., McCoy,M.T., Tice,R. and Schneider,E.L. (1988) A simple technique for the quantitation of low levels of DNA damage in individual cells. Exp. Cell Res., 175, 184-191.