Targeting of reporter molecules to mitochondria to measure calcium, ATP, and pH

Methods in Cell Biology

Volumn 65, Issue 65, 2001, Pages 353-380

  Porcelli, Anna M ^a   Pinton, Paolo ^b   Ainscow, Edward K ^c   Chiesa, Anna ^d   Rugolo, Michela ^a   Rutter, Guy A ^c   Rizzuto, Rosario ^d  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b CNR   (Italy)

^c UNIVERSITY OF BRISTOL   (United Kingdom)

^d Section of General Pathology   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; AEQUORIN; BACTERIAL PROTEIN; CALCIUM; CARBONYL CYANIDE 4 (TRIFLUOROMETHOXY)PHENYLHYDRAZONE; CHELATING AGENT; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; EGTAZIC ACID; GREEN FLUORESCENT PROTEIN; HISTAMINE; HYBRID PROTEIN; IONOPHORE; LUCIFERASE; LUCIFERIN; PHOTOPROTEIN; SIGNAL PEPTIDE; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ANIMAL; ARTICLE; CELL LINE; CHEMISTRY; DRUG EFFECT; GENETICS; HUMAN; IMAGE PROCESSING; IMMUNOHISTOCHEMISTRY; INSTRUMENTATION; INTRACELLULAR MEMBRANE; METABOLISM; METHODOLOGY; MITOCHONDRION; PH; PHYSIOLOGY; REPORTER GENE;

ADENOSINE TRIPHOSPHATE; AEQUORIN; ANIMALS; BACTERIAL PROTEINS; CALCIUM; CARBONYL CYANIDE P-TRIFLUOROMETHOXYPHENYLHYDRAZONE; CELL LINE; CHELATING AGENTS; EGTAZIC ACID; FIREFLY LUCIFERIN; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HISTAMINE; HUMANS; HYDROGEN-ION CONCENTRATION; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; INDICATORS AND REAGENTS; INTRACELLULAR MEMBRANES; IONOPHORES; LUCIFERASES; LUMINESCENT PROTEINS; MITOCHONDRIA; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS;

EID: 0035230837     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/s0091-679x(01)65021-0     Document Type: Article

References (59)

1. Aflalo, C. (1990). Targeting of cloned firefly luciferase to yeast mitochondria. Biochemistry 29, 4758-4766.
2. + channel behavior. J. Membr. Biol. 159, 161-168.
3. Bernardi, P., Scorrano, L., Colonna, R., Petronilli, V., and Di Lisa, F. (1999). Mitochondrial and cell death: Mechanistic aspects and methodological issues. Eur. J. Biochem. 264, 687-701.
4. Bowers, K. C., Allshire, A. P., and Cobbold, P. H. (1992). Bioluminescent measurement in single cardiomyocytes of sudden cytosolic ATP depletion coincident with rigor. J. Mol. Cell Cardiol. 24, 213-218.
5. c): A critical evaluation. J. Biol. Chem. 270, 9896-9903.
6. 2+ concentration measured with specifically targeted recombinant aequorin. EMBO J. 12, 4813-4819.
7. 2+ homeostasis in primary cultures of skeletal myotubes. Mol. Cell. Biol. 8, 129-143.
8. Carafoli, E. (1987). Intracellular calcium homeostasis. Annu. Rev. Biochem. 56, 395-433.
9. 2+, and volume inside cultured adult rabbit cardiac myocytes during chemical hypoxia: A multiparameter digitized confocal microscopic study. Biophys. J. 66, 942-952.
10. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., and Prasher, D. C. (1994). Green fluorescent protein as a marker for gene expression. Science 263, 802-805.
11. Contag, P. R., Olomu, I. N., Stevenson, D. K., and Contag, C. H. (1998). Bioluminescent indicators in living mammals. Nature Med. 4, 245-247.
12. Conti, E., Franks, N. P., and Brick, P. (1996). Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes. Structure 4, 287-298.
13. DeLuca, M., Wannlund, J., and McElroy, W. D. (1979). Factors affecting the kinetics of light emission from crude and purified firefly luciferase. Anal. Biochem. 95, 194-198.
14. Denton, R. M., and McCormack, J. G. (1980). On the role of the calcium transport cycle in the heart and other mammalian mitochondria. FEBS Lett. 119, 1-8.
15. De Wet, J. R., Wood, K. V., and DeLuca, M., et al. (1987). Firefly luciferase gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7, 725-737.
16. Elsliger, M.-A., Wachter, R. M., Hanson, G. T., Kallio, K., and Remington, S. J. (1999). Structural and spectral response of green fluorescent protein variants to changes in pH. Biochemistry 38, 5296-5301.
17. Gottlieb, R. A., Grauol, D. L., Zhu, J. Y., and Engler, R. L. (1996). Preconditioning rabbit cardiomyocytes: Role of pH, vacuolar proton ATPase and apoptosis. J. Clin. Invest. 97, 2391-2398.
18. Gunter, K. K., and Gunter, T. E. (1994). Transport of calcium by mitochondria. J. Bioenerg. Biomembr. 26, 471-485.
19. Halestrap, A. P. (1989). The regulation of the matrix volume of mammalian mitochondria in vivo and in vitro and its role in the control of mitochondrial metabolism. Biochim. Biophys. Acta 973, 355-382.
20. He, T. C., Zhou, S., da Costa, L. T., Yu, J., Kinzler, K. W., and Vogelstein, B. (1998). A simplified system for generating recombinant adenoviruses. Proc. Natl. Acad. Sci. USA 95, 2509-2514.
21. Isfort, R. J., Cody, D. B., Asquith, T. N., Ridder, G. M., Stuard, S. B., and LeoBoeuf, R. A. (1993). Induction of protein phosphorylation, protein synthesis: Immediate-early-gene expression and cellular proliferation by intracellular pH modulation. Implications for the role of hydrogen ions in signal transduction. Eur. J. Biochem. 213, 349-357.
22. Jouvaille, L. S., Pinton, P., Bastianutto, C., Rutter, G. A., and Rizzuto, R. (1999). Regulation of mitochondrial ATP synthesis by calcium: Evidence for a long-term metabolic priming. Proc. Natl. Acad. Sci. USA 96, 13807-13812.
23. Kennedy, H. J., Pouli, A. E., Jouvaille, L. S., Rizzuto, R., and Rutter, G. A. (1999). Glucose-induced ATP microdomains in single islet beta-cells. J. Biol. Chem. 274, 13281-13291.
24. Kneen, M., Farinas, J., Li, Y., and Verkman, A. S. (1998). Green fluorescent protein as a noninvasive intracellular pH indicator. Biophys. J. 74, 1591-1599.
25. LaNoue, K., Mizani, S. M., and Klingenberg, M. (1978). Electrical imbalance of adenine nucleotide transport across the mitochondrial membrane. J. Biol. Chem. 253, 191-198.
26. Llopis, J., McCaffery, J. M., Miyawaki, A., Farquhar, M., and Tsien, R. Y. (1998). Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. USA 95, 6803-6807.
27. Maechler, P., Wang, H., and Wollheim, C. B. (1998). Continuous monitoring of ATP levels in living insulin secreting cells expressing cytosolic firefly luciferase. FEBS Lett. 422, 328-332.
28. 2+ beneath the plasma membrane of living A7r5 cells. EMBO J. 16, 1575-1581.
29. 2+ concentration in the endoplasmic reticulum of intact cells. EMBO J. 14, 5467-5475.
30. Nicholls, D. G. (1982). "Bioenergetics: An Introduction to the Chemiosmotic Theory." Academic Press, London
31. Nicholls, D. G., and Fergusson, S. F. (1992). Quantitative bioenergetics: The measurements of driving forces In "Bioenergetics 2," p. 39-63. Academic Press, London
32. Orchard, C. H., and Kentish, J. C. (1990). Effects of changes of pH on the contractile function of cardiac muscle. Am. J. Physiol. 258, C967-C981.
33. Ormö, M., Cubitt, A. B., Kallio, Gross, L. A., Tsien, R. Y., and Remington, S. J. (1996). Crystal structure of the Aequorea victoria green fluorescent protein. Science 273, 1392-1395.
34. Orrenius, S., Burgess, D. H., Hampton, M. B., and Zhivotovsky, B. (1997). Mitochondira as the focus of apoptosis research. Cell Death Differ. 4, 427-428.
35. Patterson, G. H., Knobel, S. M., Sharif, W. D., Kain, S. R., and Piston, D. W. (1997). Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy. Biophys. J. 73, 2782-2790.
36. Pines, J. (1995). GFP in mammalian cells. Trends Genet. 11, 326-327.
37. Pinton, P., Brini, M., Bastianutto, C., Tuft, R. A., Pozzan, T., and Rizzuto, R. (1998a). New light on mitochondrial calcium. BioFactors 8, 243-253.
38. 2+ store, with distinct functional properties from the endoplasmic reticulum. EMBO J. 18, 5298-5308.
39. 2+ stores. Physiol. Rev. 74/3, 595-636.
40. 2+ concentration sensed by strategically located mitochondria. Science 262, 744-747.
41. Rizzuto, R., Brini, M., Pizzo, P., Murgia, M., and Pozzan, T. (1995). Chimeric green fluorescent protein: A new tool for visualizing subcellular organelles in living cells. Curr. Biol. 5, 635-642.
42. Rizzuto, R., Carrington, W., and Tuft, R. A. (1998a). Digital imaging microscopy of living cells. Trends Cell Biol. 8, 288-292.
43. 2+ responses. Science 280, 1763-1766.
44. 2+ revealed by specifically targeted recombinant aequorin. Nature 358, 325-328.
45. Robb-Gaspers, L. D., Burnett, P., Rutter, G. A., Denton, R. M., Rizzuto, R., and Thomas, A. P. (1998). Integrating cytosolic calcium signals into mitochondrial metabolic responses. EMBO J. 17, 4987-5000.
46. Robey, R. B., Ruiz, O., Santos, A. V. P., Ma, J., Kear, F., Wang, L.-J., Li, C.-J., Bernardo, A. A., and Arruda, J. A. L. (1998). pH-dependent fluorescence of a heterologously expressed Aequorea green fluorescent protein mutant: In situ spectral characteristics and applicability to intracellular pH estimation. Biochemistry 37, 9894-9901.
47. Roos, A., and Boron, W. F. (1981). Intracellular pH. Physiol. Rev. 61, 296-434.
48. 2+-binding to citrate cycle enzymes. Int. J. Biochem. 22, 1081-1088.
49. 2+ with recombinant targeted aequorin: Significance for the regulation of pyruvate dehydrogenase activity. Proc. Natl. Acad. Sci. USA 93, 5489-5494.
50. Rutter, G. A., Kennedy, H. J., Wood, C. D., White, M. R. H., and Tavare, J. M. (1998). Quantitative realtime imaging of gene expression in single cells using multiple luciferase reporters. Chem. Biol. 5, R285-R290. [Abstract]
51. 2+ to supramicromolar levels in a pancreatic β-cell line. J. Biol. Chem. 268, 22385-22390.
52. Rutter, G. A., White, M. R. H., and Tavare, J. M. (1995). Non-invasive imaging of luciferase gene expression in single living cells reveals the involvement of MAP kinase in insulin signalling. Curr. Biol. 5, 890-899.
53. Scholz, T. D., Laughlin, M. R., Balaban, R. S., Kupriyanov, V. V., and Heineman, F. W. (1995). Effect of substrate on mitochondrial NADH, cytosolic redox state, and phosphorylated compounds in isolated hearts. Am. J. Physiol. 268, H82-H91.
54. Soboll, S., Scholz, R., and Heldt, H. W. (1978). Subcellular metabolite concentrations: Dependence of mitochondrial and cytosolic ATP systems on the metabolic state of perfused rat liver. Eur. J. Biochem. 87, 377-390.
55. Stanley, P. E., and Williams, S. G. (1969). Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme. Anal. Biochem. 29, 381-392.
56. Tsien, R. Y. (1998). The green fluorescent protein. Annu. Rev. Biochem. 67, 509-544.
57. Ward, W. W., and Bokman, S. H. (1982). Reversible denaturation of Aequorea green fluorescent protein: Physical separation and characterization of the renatured protein. Biochemistry 21, 4535-4540.
58. White, M. R. H., Masuko, M., Amet, L., Elliott, G., Braddock, M., Kingsman, A. J., and Kingsman, S. M. (1994). Real-time analysis of the transcriptional regulation of HIV and hCMV promoters in single mammalian cells. J. Cell Sci. 108, 441-455.
59. Zhou, Y., Marcus, E. M., Haugland, R. P., and Opas, M. (1995). Use of a new fluorescent probe, seminaphthofluorescein-calcein, for determination of intracellular pH by simultaneous dual-emission imaging laser scanning confocal microscopy. J. Cell Physiol. 146, 9-16.