Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responses

Science

Volumn 280, Issue 5370, 1998, Pages 1763-1766

  Rizzuto, Rosario ^a   Pinton, Paolo ^a   Carrington, Walter ^b   Fay, Frederic S ^b   Fogarty, Kevin E ^b   Lifshitz, Lawrence M ^b   Tuft, Richard A ^b   Pozzan, Tullio ^a  

^a UNIVERSITY OF PADOVA   (Italy)

^b UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; AEQUORIN; CALCIUM ION; CARBONYL CYANIDE 4 (TRIFLUOROMETHOXY)PHENYLHYDRAZONE; GREEN FLUORESCENT PROTEIN; HISTAMINE; INOSITOL 1,4,5 TRISPHOSPHATE; MITOCHONDRIAL DNA; PHOTOPROTEIN;

ARTICLE; CHIMERA; CYTOARCHITECTURE; CYTOPLASM; DIFFUSION; ENDOPLASMIC RETICULUM; HUMAN; HUMAN CELL; MITOCHONDRION; PRIORITY JOURNAL;

ADENOSINE TRIPHOSPHATE; AEQUORIN; CALCIUM; CALCIUM CHANNELS; CELL COMPARTMENTATION; CYTOSOL; ENDOPLASMIC RETICULUM; GREEN FLUORESCENT PROTEINS; HELA CELLS; HISTAMINE; HUMANS; INOSITOL 1,4,5-TRISPHOSPHATE; INTRACELLULAR MEMBRANES; ION CHANNEL GATING; LUMINESCENT PROTEINS; MITOCHONDRIA; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION; TRANSFECTION;

EID: 0032511112     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5370.1763     Document Type: Article

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31. The ER and mitochondria images were separately thresholded over a range that eliminated background but preserved organellar structure. Subresolution functional overlap was determined from the percentage of mitochondria surface voxels that also contained ER; compensating for the limited resolution (which increases apparent overlap) increased the range of estimated overlap.
32. 2+ uniporter is located.
33. 2+ uniporter is located.
34. 2+ uniporter is located.
35. 2+ uniporter is located.
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39. 2+], ionic strength, and temperature (12).
40. c (14), was delayed.
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42. 2+] as cAEQ.
43. 2+] in these microdomains was high enough to cause complete discharge of all their aequorin content, the regions should correspond to ~∼ to 5% of the MIMS.
44. We thank M. Murgia for constructing the erGFP(S65T) cDNA; R. Bisson, F. DiLisa, C. Montecucco, and G. Rutter for critically reading and discussing the manuscript; and J. Carmichael, G. Ronconi, and M. Santato for technical assistance. Supported by grants from Telethon (845, 850), from the Human Frontier Science Program, from the E. U. Biomed program (BMH4CT960181), from the Armenise Foundation (Harvard University), from the Italian University Ministry, and from the British Research Council to R.R. and T.P., by grants from NSF (DBI-9200027 and DBI-9724611) and NIH (HL14523 and RR09799) to W.C., F.S.F., K.E.F., L.M.L., and R.A.T.; and by a NATO Travel grant to R.R. and F.S.F. This report is dedicated to the memory of our dear friend and colleague Fredric S. Fay, whose enthusiasm and insights nurtured this collaboration, but who did not live to see its fruition.