Domains of high Ca2+ beneath the plasma membrane of living A7r5 cells

EMBO Journal

Volumn 16, Issue 7, 1997, Pages 1575-1581

  Marsault, Robert ^a   Murgia, Marta ^a   Pozzan, Tullio ^a   Rizzuto, Rosario ^a  

^a UNIVERSITY OF PADOVA   (Italy)

Author keywords

Calcium; Plasma membrane; Recombinant aequorin; Targeting

Indexed keywords

AEQUORIN; CALCIUM CHANNEL; CALCIUM ION; CELL MEMBRANE PROTEIN; PHOTOPROTEIN; CALCIUM; EGTAZIC ACID; HYBRID PROTEIN; INOSITOL 1,4,5 TRISPHOSPHATE; MEMBRANE PROTEIN; NERVE PROTEIN; PRIMER DNA; SNAP25 PROTEIN, RAT; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; VASOPRESSIN DERIVATIVE;

ANIMAL CELL; AORTA MEDIA; ARTICLE; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CELL MEMBRANE; CELL ORGANELLE; CHIMERA; CYTOPLASM; CYTOSOL; IMMUNOCYTOCHEMISTRY; MUSCLE FIBER CULTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROCESSING; PROTEIN PROTEIN INTERACTION; PROTEIN TARGETING; RAT; SYNAPTOSOME; ANIMAL; AORTA; BIOSYNTHESIS; CELL LINE; GENETIC TRANSFECTION; KINETICS; METABOLISM; SITE DIRECTED MUTAGENESIS; VASCULAR SMOOTH MUSCLE;

AEQUORIN; ANIMALS; AORTA; CALCIUM; CELL LINE; CELL MEMBRANE; CYTOSOL; DNA PRIMERS; EGTAZIC ACID; INOSITOL 1,4,5-TRISPHOSPHATE; KINETICS; MEMBRANE PROTEINS; MUSCLE, SMOOTH, VASCULAR; MUTAGENESIS, SITE-DIRECTED; NERVE TISSUE PROTEINS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RATS; RECOMBINANT FUSION PROTEINS; SYNAPTOSOMAL-ASSOCIATED PROTEIN 25; TRANSFECTION; VASOPRESSINS;

EID: 0031000753     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.7.1575     Document Type: Article

References (27)

1. 2+ concentration measured with specifically targeted recombinant aequorin. EMBO J., 12, 4813-4819.
2. 2+]). A critical evaluation. J. Biol. Chem., 270, 9896-9903.
3. 2+ efflux in A7r5 rat smooth muscle cells. J. Physiol., 485, 455-468.
4. Clapham,D.E. (1995) Calcium signaling. Cell, 80, 259-268
5. 2+ indicator. J. Biol. Chem., 13, 10141-10149.
6. 2+ indicator FFP18. Proc. Natl Acad. Sci. USA, 93, 5368-5373.
7. 2+ influx - how many mechanisms for how many channels? Trends Pharmacol. Sci., 15, 77-83.
8. Field,J., Nikawa,J., Broek,D., MacDonald,B., Rodgers,L., Wilso,I.A., Lerner,R.A. and Wigler,M. (1988) Purification of a RAS-responsive adenyl-cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol. Cell. Biol., 8, 2159-2165.
9. 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem., 260, 3440-3450.
10. Heidelberger,R., Heinemann,C. Neher,E. and Matthews,G. (1994) Calcium dependence of the rate of exocytosis in a synaptic terminal. Nature, 371, 513-515.
11. Hess,D.T., Slater,T.M., Wilson,M.C. and Skene,J.H.P. (1992) The 25 kDa synaptosomal-associated protein SNAP-25 is the major methionine-rich polypeptide in rapid axonal transport and a major substrate for palmitoylation in adult CNS. J. Neurosci., 12, 4634-4641.
12. Hille,B., Woodhull,A.M. and Shapiro,B.I. (1975) Negative surface charge near sodium channels of nerve: divalent ions, monovalent ions, and pH. Philos. Trans. R. Soc. Lond. B., 270, 301-318.
13. Hoth,M. and Penner,R. (1992) Depletion of intracellular calcium stores activates a calcium current in mast cells. Nature, 355, 353-356.
14. Hoth,M. and Penner,R. (1993) Calcium release-activated calcium current in rat mast cells. J. Physiol., 465, 359-386.
15. Inouye,S., Noguchi,M., Sakaki,Y., Takagi,Y., Miyata,T., Iwanaga,S., Miyata,T. and Tsuji,F.I. (1985) Cloning and sequence analysis of cDNA for the luminescent protein aequorin. Proc. Nail Acad. Sci. USA, 82, 3154-3158.
16. Kimes,B.W. and Brandt,B.C. (1976) Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp. Cell Res., 98, 349-366.
17. Llinás,R., Sugimori,M. and Silver,R.B. (1992) Microdomains of high calcium concentration in a presynaptic terminal. Science, 256, 677-679.
18. Llinás,R., Sugimori,M. and Silver,R.B. (1995) The concept of calcium concentration microdomains in synaptic transmission. Neuropharmacology, 34, 1443-1451.
19. 2+ concentration in the endoplasmic reticulum of intact cells. EMBO J., 14, 5467-5475.
20. 2+ revealed by specifically targeted recombinant aequorin. Nature, 358, 325-328.
21. 2+ close to IP3-sensitive channels that are sensed by neighboring mitochondria. Science, 262, 744-747.
22. 2+ homeostasis in intact cells. J. Cell Biol., 126, 1183-1194.
23. Rizzuto,R., Brini,M., Bastianutto,C., Marsault,R. and Pozzan,T. (1995) Photoprotein-mediated measurement of calcium ion concentration in mitochondria of living cells. Methods Enzymol., 260, 417-428.
24. 2+-dependent aequorin luminescence of microdomains and QEDs in synaptic preterminals. Biol. Bull., 187, 293-299.
25. 2+ chelating agent. Biochem. Biophys. Res. Commun., 218, 837-841.
26. + channel in aortic smooth muscle cells (A7r5 line and primary culture). Plügers Arch., 420, 417-423.
27. Zweifach,A. and Lewis,R.S. (1995) Rapid inactivation of depletion-activated calcium current (Icrac) due to local calcium feedback. J. Gen. Physiol., 105, 209-226.