Real-time imaging of gene expression in single living cells

Chemistry and Biology

Volumn 5, Issue 11, 1998, Pages

  Rutter, Guy A ^a   Kennedy, Helen J ^a   Wood, Christopher D ^b   White, Michael R H ^b   Tavaré, Jeremy M ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

^b UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE; GENE EXPRESSION; GENETIC TRANSCRIPTION; IMAGING; LIVING CELL; LUMINESCENCE; REPORTER GENE;

EID: 0032213851     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(98)90287-3     Document Type: Article

References (27)

1. Ormo, M., Cubitt, A.B., Kallio, K., Gross, L.A., Tsien, R.Y. & Remington, S.J. (1996). Crystal structure of the Aequorea victoria green fluorescent protein. Science 273, 1392-1395.
2. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W. & Prasher, D.C. (1994). Green fluorescent protein as a marker for gene expression. Science 263, 802-805
3. Heim, R. & Tsien, R.Y. (1996). Engineering green fluorescent protein for improved brightness, longer wavelengths & fluorescence resonance energy transfer. Curr. Biol. 6, 178-182.
4. Rizzuto, R., Brini, M., Pizzo, P., Murgia, M. & Pozzan, T. (1995). Chimeric green fluorescent protein as a tool for visualising subcellular organelles in living cells. Curr. Biol. 5, 635-642.
5. Venkateswarlu, K., Oatey, P.B., Tavaré, J.M. & Cullen, P.J.(1998). Insulin-dependent translocation of ARNO to the plasma membrane of adipocytes requires phosphatidylinositol 3-kinase. Curr. Biol. 8, 463-466.
6. Pouli, A.E., Emmanouilidou, E., Zhao, C., Wasmeier, C., Hutton, J.C. & Rutter, G.A. (1998). Secretory granule dynamics visualised in vivo with a phogrin-green fluorescent protein chimaera. Biochem. J. 333, 193-199.
7. Kaether, C., Salm, T., Glombik, M., Almers, W. & Gerdes, H.H. (1997). Targeting of green fluorescent protein to neuroendocrine secretory granules: a new tool for real time studies of regulated protein secretion. Eur. J. Cell Biol. 74, 133-142.
8. Ludin, B. & Matus, A. (1998). GFP illuminates the cytoskeleton. Trends Cell Biol. 8, 72-77.
9. Siemering, K.R., Golbik, R., Sever, R. & Haseloff, J. (1996). Mutations that suppress the thermosensitivity of green fluorescent protein. Curr. Biol. 6, 1653-1663.
10. Rutter, G.A., White, M.R.H. & Tavaré, J.M. (1995). Non-invasive imaging of luciferase gene expression in single living cells reveals the involvement of MAP kinase in insulin signalling. Curr. Biol. 5, 890-899.
11. deVargas, L.M., Sobolewski, J., Siegel, R. & Moss, L.G. (1997). Individual beta cells within the intact islet differentially respond to glucose. J. Biol. Chem. 272, 26573-26577.
12. Leibiger, I.B., Leibiger, B., Moede, T. & Berggren, P.O. (1998). Exocytosis of insulin promotes insulin gene transcription via the insulin receptor PI-3 kinase p70 s6 kinase and CaM kinase pathways. Mol. Cell 1, 933-938.
13. 2+ based on green fluorescent proteins and calmodulin. Nature 388, 882-887.
14. Zlokarnik, G., et al., & Tsien, R.Y. (1998). Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter. Science 279, 84-88.
15. 2+ spike frequency can optimize gene expression. Nature 392, 936-941.
16. deWet, J., Wood, K.V., DeLuca, M., Helinski, D.R. & Subramani, S. (1987). Firefly luciferase gene: structure and expression in mammalian cells. Mol. Cell. Biol. 7, 725-737.
17. K.V. Wood (1995). Marker proteins for gene expression. Curr. Opin. Biotechnol. 6, 50-58.
18. Lorenz, W.W., McCann, R.O., Longiaru, M. & Cormier, M.J. (1991) Isolation and expression of a cDNA encoding Renilla reniformis luciferase. Proc. Natl Acad Sci. USA 88, 4438-4442.
19. Tavaré, J.M. & Rutter, G.A. (1998). Luminescence imaging of regulated gene expression in single living cells. In Fluorescent Imaging of Optical Probes. (Mason, W.T., ed.), Academic Press, in press.
20. White, M.R.H., et al., & Chiswell, D.J. (1990). Imaging of firefly luciferase expression in single mammalian cells using high sensitivity charge coupled device cameras. Technique 2, 194-201.
21. Craig, F.F., Simmonds, A.C., Watmore, D., McCapra F. & White, M.R.H. (1991). Membrane permeable luciferin esters for assay of firefly luciferase in live intact cells. Biochem. J. 276, 637-641.
22. Takasuka, N., White, M.R, Wood, C.D., Robertson, W.R. & Davis, J.R. (1998). Dynamic changes in prolactin promoter activation in individual living lactotrophic cells. Endocrinol. 139, 1361-1368.
23. Kennedy, H.J., Viollet, B., Rafiq, I., Kahn, A. & Rutter, G.A. (1997). Upstream stimulatory factor-2 (USF2) activity is required for glucose stimulation of L-pyruvate kinase promoter activity in single living islet beta cells. J. Biol. Chem. 272, 20636-20640.
24. Arlan, T., Mamaev, S.V. Mamaeva, N.V. & Hecht, SM (1997). Structurally modified luciferase, effects of amino acid substitution at position 286. J. Am. Chem. Soc, 119, 10877-10887.
25. Brandes, C., et al., & Hall, J.C. (1996). Novel features of Drosophila period transcription revealed by real-time luciferase reporting. Neuron 16, 687-692.
26. Contag, C.H., et al., & Benaron, DA (1997), Visualizing gene expression in living mammals using a bioluminescent reporter. Photochem. Photobiol. 66, 523-531.
27. Niswender, K.D., Blackman, S.M., Rohde, L., Magnuson, M.A. & Piston, D.W. (1995). Quantitative imaging of green fluorescent protein in cultured cells: comparison of microscopic techniques, use in fusion proteins and detection limits. J. Microsc. 180, 109-116.