Exchange of the basic domain of human immunodeficiency virus type 1 Rev for a polyarginine stretch expands the RNA binding specificity, and a minimal arginine cluster is required for optimal RRE RNA binding affinity, nuclear accumulation, and trans-activation

Journal of Virology

Volumn 75, Issue 6, 2001, Pages 2957-2971

  Nam, Y S ^a   Petrovic, A ^a   Jeong, K S ^a   Venkatesan, S ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COAT PROTEIN; LEPTOMYCIN B; POLYARGININE; REGULATOR PROTEIN; REV PROTEIN; STRUCTURAL PROTEIN;

ARTICLE; BINDING AFFINITY; HUMAN IMMUNODEFICIENCY VIRUS 1; INTRACELLULAR TRANSPORT; MS2 VIRUS; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; OLIGOMERIZATION; PHENOTYPE; PRIORITY JOURNAL; PROTEIN ANALYSIS; RNA BINDING; TRANSACTIVATION; VIRUS MORPHOLOGY;

ANIMALS; ARGININE; CAPSID; CAPSID PROTEINS; CELL LINE; CELL NUCLEUS; GENE PRODUCTS, REV; GENES, ENV; GENES, REV; HIV-1; HUMANS; MULTIGENE FAMILY; MUTAGENESIS, INSERTIONAL; NUCLEAR LOCALIZATION SIGNALS; PEPTIDES; PLASMIDS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; RNA, VIRAL; RNA-BINDING PROTEINS; TRANS-ACTIVATION (GENETICS);

EID: 0035120436     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.6.2957-2971.2001     Document Type: Article

References (99)

1. The specificity of the CRMI-Rev nuclear export signal interaction is mediated by RanGTP
2. HIV-1 Rev regulation involves recognition of non-Watson-Crick base pairs in viral RNA
3. Alpha helix-RNA major groove recognition in an HIV-1 rev peptide-RRE RNA complex
4. Binding of an HIV Rev peptide to Rev responsive element RNA induces formation of purine-purine base pairs
5. Inhibition of human immunodeficiency virus type 1 replication in human T cells by retroviral-mediated gene transfer of a dominant-negative Rev trans-activator
6. Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins
7. Functional mapping of the human immunodeficiency virus type 1 Rev RNA binding domain: New insights into the domain structure of Rev and Rex
8. Transdominant repressors for human T-cell leukemia virus type 1 Rex and human immunodeficiency virus type 1 Rev function
9. Analysis of arginine-rich peptides from the HIV Tat protein reveals unusual features of RNA-protein recognition
10. Arginine-mediated RNA recognition: The arginine fork
11. Human immunodeficiency virus type 1 Rev is required in vivo for binding of poly(A)-binding protein to Rev-dependent RNAs
12. Sequence specific interaction of R17 coat protein with its ribonucleic acid binding site
13. Regulation by HIV Rev depends upon recognition of splice sites
14. The basic RNA-binding domain of HIV-2 Tat contributes to preferential trans-activation of a TAR2-containing LTR
15. Identification of sequences important in the nucleolar localization of human immunodeficiency virus Rev: Relevance of nucleolar localization to function
16. Characterization of HIV-1 REV protein: Binding stoichiometry and minimal RNA substrate
17. Specific binding of HIV-1 recombinant Rev protein to the Rev-responsive element in vitro
18. The amino terminal domain of HIV-1 Rev is required for discrimination of the RRE from nonspecific RNA
19. The rev gene product of the human immunodeficiency virus affects envelope-specific RNA localization
20. RevM10-mediated inhibition of HIV-1 replication in chronically infected T cells
21. HTLV III expression and production involve complex regulation at the levels of splicing and translation of viral RNA
22. Rev protein of human immunodeficiency virus type 1 affects the stability and transport of the viral mRNA
23. CRM1 is an export receptor for leucine-rich nuclear export signals
24. Site-specific phosphorylation of the human immunodeficiency virus type-1 Rev protein accelerates formation of an efficient RNA-binding conformation
25. A human nucleoporin-like protein that specifically interacts with HIV Rev
26. CRM1 is responsible for intracellular transport mediated by the nuclear export signal
27. The interaction between HIV-1 Tat and human cyclin T1 requires zinc and a critical cysteine residue that is not conserved in the murine CycT1 protein
28. The refined structure of bacteriophage MS2 at 2.8 Å resolution
29. A 41 amino acid motif in importin-alpha confers binding to importin-beta and hence transit into the nucleus
30. The Rev (Trs/Art) protein of human immunodeficiency virus type 1 affects viral mRNA and protein expression via cis-acting sequences in the env region
31. Regulation of human immunodeficiency virus env expression by the rev gene product
32. Scanning mutagenesis of the arginine-rich region of the human immunodeficiency virus type 1 Rev trans-activator
33. HIV-1 regulator of virion expression (Rev) binds to an RNA stem-loop structure located in the Rev response element region
34. Human immunodeficiency virus type 1 regulator of virion expression, rev forms nucleoprotein filaments after binding to a purine-rich "bubble" located within the rev-responsive region of viral mRNAs
35. Interactions between HIV Rev and nuclear import and export factors: The Rev nuclear localisation signal mediates specific binding to human importin-beta
36. Human immunodeficiency virus Rev protein recognizes a target sequence in Rev-responsive element RNA within the context of RNA secondary structure
37. A specific sequence with a bulged guanosine residue(s) in a stem-bulge-stem structure of Rev-responsive element RNA is required for trans activation by human immunodeficiency virus type 1 Rev
38. Effector domains of human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type 1 Rex are functionally interchangeable and share an essential peptide motif
39. Steroid-receptor fusion of the human immunodeficiency virus type 1 Rev transactivalor: Mapping cryptic functions of the arginine-rich motif
40. trans-Dominant inhibition of human immunodeficiency virus type 1 Rev occurs through formation of inactive protein complexes
41. Minimal Rev-responsive element for type 1 human immunodeficiency virus
42. Deletions near the N-terminus of HIV-1 Rev reduce RNA binding affinity and dominantly interfere with Rev function irrespective of the RNA target
43. The human immunodeficiency virus type 1 Rev protein shuttles between the cytoplasm and nuclear compartments
44. Structural analysis of the interaction between the human immunodeficiency virus Rev protein and the Rev response element
45. Specific binding of a basic peptide from HIV-1 Rev
46. Expression of the art/trs protein of HIV and study of its role in viral envelope synthesis
47. Inhibition of human immunodeficiency virus type 1 Rev function by a Rev mutant which interferes with nuclear/nucleolar localization of Rev
48. Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region
49. Leptomycin B inhibition of signal-mediated nuclear export by direct binding to CRM1
50. Reconstitution of HIV-1 rev nuclear export: Independent requirements for nuclear import and export
51. Sequence requirements for Rev multimerization in vivo
52. Functional dissection of the HIV-1 Rev trans-activator-derivation of a transdominant repressor of Rev function
53. The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA
54. HIV-1 structural gene expression requires the binding of multiple Rev monomers to the viral RRE: Implications for HIV-1 latency
55. Rev and the fate of pre-mRNA in the nucleus: Implications for the regulation of RNA processing in eukayotes
56. Stable expression of transdominant Rev protein in human T cells inhibits human immunodeficiency virus replication
57. Mutational definition of the human immunodeficiency virus type 1 Rev activation domain
58. HIV-1 structural gene expression requires binding of the Rev trans-activator to its RNA target sequence
59. A molecular rheostat. Co-operative rev binding to stem I of the rev-response element modulates human immunodeficiency virus type-1 late gene expression
60. Posttranscriptional regulation by the human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type 1 Rex proteins through a heterologous RNA binding site
61. Identification of trans-dominant HIV-1 rev protein mutants by direct transfer of bacterially produced proteins into human cells
62. The HIV-1 Rev trans-activator shuttles between the nucleus and the cytoplasm
63. Crystal structure of the MS2 coat protein dimer: Implications for RNA binding and virus assembly
64. Mutational analysis of the HIV-1 Rev protein and its target sequence, the Rev responsive element
65. Interaction of the human immunodeficiency virus type 1 Rev protein with a structured region in env mRNA is dependent on multimer formation mediated through a basic stretch of amino acids
66. Evidence for a role of CRM1 in signal-mediated nuclear protein export
67. Importin beta can mediate the nuclear import of an arginine-rich nuclear localization signal in the absence of importin alpha
68. TAR RNA-binding protein is an inhibitor of the interferon-induced protein kinase PKR
69. The RNA binding site of bacteriophage MS2 coat protein
70. Biochemical and functional interactions between HIV-1 Tat protein and TAR RNA
71. HIV-1 Rev is capable of shuttling between the nucleus and cytoplasm
72. RNA binding site of R17 coat protein
73. Intragenic cis-acting art gene-responsive sequences of the human immunodeficiency virus
74. Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activation
75. A second post-transcriptional trans-activator gene required for HTLV-III replication
76. Exportin I (Crm1p) is an essential nuclear export factor
77. RNA recognition by an isolated alpha helix
78. Costabilization of peptide and RNA structure in an HIV Rev peptide-RRE complex
79. Specific binding of arginine to TAR RNA
80. Probing the structure of the HIV-1 Rev trans-activator protein by functional analysis
81. Functional analysis of the human immunodeficiency virus type 1 Rev protein oligomerization interface
82. Association of the T-cell protein tyrosine phosphatase with nuclear import factor p97
83. Identification of a high-affinity RNA-binding site for the human immunodeficiency virus type 1 Rev protein
84. The arginine-rich domains present in human immunodeficiency virus type 1 Tat and Rev function as direct importin beta-dependent nuclear localization signals
85. + RNA binding protein
86. The three-dimensional structure of the bacterial virus MS2
87. Real-time kinetics of HIV-1 Rev-Rev response element interactions. Definition of minimal binding sites on RNA and protein and stoichiometric analysis
88. Human immunodeficiency virus type 1 Rev activation can be achieved without Rev-responsive element RNA if Rev is directed to the target as a Rev/MS2 fusion protein which tethers the MS2 operator RNA
89. Mutants in a conserved region near the carboxy-terminus of HIV-1 Rev identify functionally important residues and exhibit a dominant negative phenotype
90. A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA
91. Definition of the human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type 1 Rex protein activation domain by functional exchange
92. The conserved amino-terminal domain of hSRP1 alpha is essential for nuclear protein import
93. HIV-1 Rev expressed in recombinant Escherichia coli: Purification, polymerization, and conformational properties
94. Specific interaction between RNA phage coat proteins and RNA
95. Cooperative binding of R17 coat protein to RNA
96. Nucleocytoplasmic transport of the Rev protein of human immunodeficiency virus type 1 is dependent on the activation domain of the protein
97. Leptomycin B is an inhibitor of nuclear export: Inhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNA
98. Sequence-specific RNA binding by the HIV-1 Rev protein
99. Oligomerization and RNA binding domains of the type 1 human immunodeficiency virus Rev protein: A dual function for an arginine-rich binding motif