NXT1 (p15) is a crucial cellular cofactor in TAP-dependent export of intron-containing RNA in mammalian cells

Molecular and Cellular Biology

Volumn 21, Issue 7, 2001, Pages 2545-2554

  Guzik, B W ^a   Levesque, L ^a   Prasad, S ^a   Bor, Y C ^a   Black, B E ^a   Paschal, B M ^a   Rekosh, D ^a   Hammarskjold M L ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHIMERIC PROTEIN; FUNGAL PROTEIN; GENE PRODUCT; LEPTOMYCIN B; MESSENGER RNA; MUTANT PROTEIN; NUCLEOPORIN; PROTEIN NXT1; PROTEIN P15; UNCLASSIFIED DRUG; VIRUS RNA; VIRUS VECTOR;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; DNA RESPONSIVE ELEMENT; GENETIC TRANSFECTION; HUMAN IMMUNODEFICIENCY VIRUS; INTRON; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN INTERACTION; RNA BINDING; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION;

ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; CELL LINE; HUMANS; INTRONS; MUTATION; NUCLEAR PROTEINS; NUCLEOCYTOPLASMIC TRANSPORT PROTEINS; RNA; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS;

ANIMALIA; HUMAN IMMUNODEFICIENCY VIRUS; MAMMALIA; RNA VIRUSES;

EID: 0035102745     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.21.7.2545-2554.2001     Document Type: Article

References (66)

1. RanGTP-regulated interactions of CRM1 with nucleoporins and a shuttling DEAD-box helicase
2. The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates
3. Identification of novel import and export signals of human TAP, the protein that binds to the constitutive transport element of the type D retrovirus mRNAs
4. Secreted placental alkaline phosphatase: A powerful new quantitative indicator of gene expression in eukaryotic cells
5. Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export
6. Inhibition of human immunodeficiency virus Rev and human T-cell leukemia virus Rex function, but not Mason-Pfizer monkey virus constitutive transport element activity, by a mutant human nucleoporin targeted to Crml
7. TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus
8. A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent
9. Retroviruses as model systems for the study of nuclear RNA export pathways
10. Secreted placental alkaline phosphatase as a eukaryotic reporter gene
11. Specific binding of HIV-1 recombinant Rev protein to the Rev-responsive element in vitro
12. Location of the HIV-1 Rev protein during mitosis: Inactivation of the nuclear export signal alters the pathway for postmitotic reentry into nucleoli
13. Secondary structure and mutational analysis of the Mason-Pfizer monkey virus RNA constitutive transport element
14. A structured retroviral RNA element that mediates nucleocytoplasmic export of intron-containing RNA
15. The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs
16. CRM1 is an export receptor for leucine-rich nuclear export signals
17. The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component, Nup88
18. Amphibian transcription factor IIIA proteins contain a sequence element functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev
19. Transformation of rat cells by DNA of human adenovirus 5
20. TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus
21. Monomethylated cap structures facilitate RNA export from the nucleus
22. Regulation of retroviral RNA export
23. Regulation of human immunodeficiency virus env expression by the rev gene product
24. Human immunodeficiency virus env expression becomes Rev-independent if the env region is not defined as an intron
25. High-level expression of the Epstein-Barr virus EBNA1 protein in CV1 cells and human lymphoid cells using a SV40 late replacement vector
26. Site-directed mutagenesis by overlap extension using the polymerase chain reaction
27. In or out? Regulating nuclear transport
28. Effector domains of human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex are functionally interchangeable and share an essential peptide motif
29. Nuclear export of different classes of RNA is mediated by specific factors
30. Analysis of cellular factors that mediate nuclear export of RNAs bearing the Mason-Pfizer monkey virus constitutive transport element
31. The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences
32. The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human
33. Nuclear transport and transcription
34. Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm
35. Identification of a cis-acting element in human immunodeficiency virus type 2 (HIV-2) that is responsive to the HIV-1 rev and human T-cell leukemia virus types I and II rex proteins
36. Functional dissection of the HIV-1 Rev trans-activator - Derivation of a trans-dominant repressor of Rev function
37. Rev and the fate of pre-mRNA in the nucleus: Implications for the regulation of RNA processing in eukaryotes
38. The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA
39. Nucleocytoplasmic transport: The soluble phase
40. The HIV-1 Rev trans-activator shuttles between the nucleus and the cytoplasm
41. Purification of a Ran-interacting protein that is required for protein import into the nucleus
42. The NES-Crm1p export pathway is not a major mRNA export route in Saccharomyces cerevisiae
43. The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export
44. Avian retroviral RNA element promotes unspliced RNA accumulation in the cytoplasm
45. Expression and purification of the HIV type 1 Rev protein produced in Escherichia coli and its use in the generation of monoclonal antibodies
46. RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro
47. Leptomycin B inhibits equine infectious anemia virus Rev and feline immunodeficiency virus Rev function but not the function of the hepatitis B virus posttranscriptional regulatory element
48. Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62
49. The constitutive transport element (CTE) of Mason-Pfizer monkey virus (MPMV) accesses a cellular mRNA export pathway
50. The HIV-1 Rev protein
51. The simian retrovirus-1 constitutive transport element, unlike the HIV-1 RRE, uses factors required for cellular mRNA export
52. Distinct RNA sequences in the gag region of human immunodeficiency virus type I decrease RNA stability and inhibit expression in the absence of Rev protein
53. + RNA and nuclear pores
54. gag-pol expressed from a simian virus 40 late replacement vector are efficiently processed and assembled into viruslike particles
55. gag-pol from human immunodeficiency virus type 1 into virus-like particles
56. The effect of viral regulatory protein expression on gene delivery by human immunodeficiency virus type 1 vectors produced in stable packaging cell lines
57. Exportin 1 (Crm1p) is an essential nuclear export factor
58. Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export
59. REF, an evolutionary conserved family of hnRNP-like proteins, interacts with TAP/Mex67p and participates in mRNA nuclear export
60. Nuclear-cytoplasmic shuttling of C-ABL tyrosine kinase
61. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor
62. Leptomycin B is an inhibitor of nuclear export: Inhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNA
63. Tap: A novel cellular protein that interacts with tip of herpesvirus saimiri and induces lymphocyte aggregation
64. Nucleoporins Nup98 and Nup214 participate in nuclear export of human immunodeficiency virus type 1 Rev
65. Continuous propagation of RRE(-) and Rev(-)RRE(-) human immunodeficiency virus type 1 molecular clones containing a cis-acting element of simian retrovirus type 1 in human peripheral blood lymphocytes