The aspartic proteinase is expressed in Arabidopsis thaliana seeds and localized in the protein bodies

Seed Science Research

Volumn 9, Issue 1, 1999, Pages 75-84

  Mutlu, Asuman ^a,b   Chen, Xia ^a   Reddy, Sridhar M ^a   Gal, Susannah ^a  

^a BINGHAMTON UNIVERSITY   (United States)

^b HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

2S albumin; In situ hybridization; Probarley lectin; Processing protease; Protein precursor; Protein storage vacuoles; Storage protein

Indexed keywords

ALPHA MANNOSIDASE; ASPARTIC PROTEINASE; ELECTRON MICROSCOPY; ENZYME IMMOBILIZATION; ENZYME LOCALIZATION; IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNOCYTOCHEMISTRY; NORTHERN BLOTTING; PROTEIN EXPRESSION;

ARABIDOPSIS THALIANA;

EID: 0032933405     PISSN: 09602585     EISSN: None     Source Type: Journal    
DOI: 10.1017/s0960258599000082     Document Type: Article

References (41)

1. Bethke, P.C., Hillmer, S. and Jones, R.L. (1996) Isolation of intact protein storage vacuoles from barley aleurone: identification of aspartic and cystein proteases. Plant Physiology 110, 521-529.
2. Boller, T. and Kende, H. (1979) Hydrolytic enzymes in the central vacuole of plant cells. Plant Physiology 63, 1123-1132.
3. Callis, J. (1995) Regulation of protein degradation. Plant Cell 7, 845-857.
4. Chen, F. and Foolad, M.R. (1997) Molecular organization of a gene in barley which encodes a protein similar to aspartic protease and its specific expression in nucellar cells during degeneration. Plant Molecular Biology 35, 821-831.
5. Conceição, A.d.S. and Krebbers, E. (1994) A cotyledon regulatory region is responsible for the different spatial expression patterns of Arabidopsis 2S albumin genes. Plant Journal 5, 493-505.
6. Cordeiro, M.C., Pais, M.S. and Brodelius, P.E. (1994) Tissue-specific expression of multiple forms of cyprosin (aspartic proteinase) in flowers of Cynara cardunculus. Physiologia Plantarum 92, 645-653.
7. D'Hondt, K., Stack, S., Gutteridge, S., Vandekerckhove, J., Krebbers, E. and Gal., S. (1997) Aspartic proteinase genes in the Brassicaceae Arabidopsis thaliana and Brassica napus. Plant Molecular Biology 33, 187-192.
8. D'Hondt, K., Bosch, D., Damme, J.F., Goethals, M., Vandekerckhove, J. and Krebbers, E. (1993) An aspartic proteinase present in seeds cleaves Arabidopsis 2S albumin precursors in vitro. Journal of Biological Chemistry 268, 20884-20891.
9. De Block, M. and Debrouwer, D. (1993) RNA-RNA in situ hybridization using digoxigenin-labeled probes: the use of high-molecular-weight polyvinyl alcohol in the alkaline phosphatase indoxylnitroblue tetrazolium reaction. Analytical Biochemistry 215, 86-89.
10. De Clercq, A., Vandewiele, M., Van Damme, J., Guerche, P., Van Montagu, M., Vandekerckhove, J. and Krebbers, E. (1990) Stable accumulation of modified 2S albumin seed storage proteins with higher methionine contents in transgenic plants. Plant Physiology 94, 970-979.
11. Dunaevsky, Y.E., Sarbakanova, S.T. and Belozersky, M.A. (1989) Wheat seed carboxypeptidase and joint action on gliadin of proteases from dry and germinating seeds. Journal of Experimental Botany 40, 1323-1329.
12. Fujikura, Y. and Karssen, C.M. (1995) Molecular studies on osmoprimed seeds of cauliflower: a partial amino acid sequence of a vigour-related protein and osmopriming-enhanced expression of a putative aspartic protease. Seed Science Research 5, 177-181.
13. Gu, J., Stephenson, C.G. and Iadarola, M.J. (1994) Recombinant proteins attached to a Nickel-NTA column: use in affinity purification of antibodies. BioTechniques 17, 257-262.
14. Hara-Nishimura, I. and Nishimura, M. (1987) Proglobulin processing enzyme in vacuoles isolated from developing pumpkin cotyledons. Plant Physiology 85, 440-445.
15. Hara-Nishimura, I., Shimada, T., Hiraiwa, N. and Nishimura, M. (1995) Vacuolar processing enzyme responsible for maturation of seed proteins. Journal of Plant Physiology 145, 632-640.
16. Higgins, T.J.V. (1984) Synthesis and regulation of major proteins in seeds. Annual Review of Plant Physiology 35, 191-221.
17. Hiraiwa, N., Takeuchi, Y., Nishimura, M. and Hara-Nishimura, I. (1993) A vacuolar processing enzyme in maturing and germinating seeds: its distribution and associated changes during development. Plant Cell Physiology 34, 1197-1204.
18. Hiraiwa, N., Kondo, M., Nishimura, M. and Hara-Nishimura, I. (1997) An aspartic endopeptidase is involved in the breakdown of propeptides of storage proteins in protein-storage vacuoles of plants. European Journal of Biochemistry 246, 133-141.
19. Jackson, D. (1991) In situ hybridization in plants. pp. 163-174 in Gurr, S.J.; McPherson, M.J.; Bowles, D.J. (Eds) Molecular Plant Pathology: A Practical Approach. Oxford, Oxford University Press.
20. Krebbers, E., Herdies, L., De Clerq, A., Seurinck, J., Leemans, J., Van Damme, J., Segura, M., Gheysen, G., Van Montagu, M. and Vandekerckhove, J. (1988) Determination of the processing sites of an Arabidopsis 2S albumin and characterization of the complete gene family. Plant Physiology 87, 859-866.
21. Marttila, S., Jones, B.L. and Mikkonen, A. (1995) Differential localization of two acid proteinases in germinating barley (Hordeum vulgare) seed. Physiologia Plantamm 93, 317-327.
22. Mutlu, A., Pfeil, J. and Gal, S. (1998) A probarley lectin processing enzyme purified from Arabidopsis thaliana seeds. Phytochemistry 47, 1453-1459.
23. Mutlu, A. and Gal, S. (1999) Plant aspartic proteinases: enzymes on the way to a function. Physiologia Plantarum, in press.
24. Nishimura, M. (1982) pH in vacuoles isolated from castor bean endosperm. Plant Physiology 70, 742-744.
25. Pang, P.P., Pruitt, R.E. and Meyerowitz, E.M. (1988) Molecular cloning, genomic organization, expression and evolution of 12S seed storage protein genes of Arabidopsis thaliana. Plant Molecular Biology 11, 805-820.
26. Paris, N., Stanley, C.M., Jones, R.L. and Rogers, J.C. (1996) Plant cells contain two functionally distinct vacuolar compartments. Cell 85, 563-572.
27. Pernollet, J.C. (1978) Protein bodies of seeds: ultrastructure, biochemistry, biosynthesis, and degradation. Phytochemistry 17, 1473-1480.
28. Qi, X., Wilson, K.A. and Tan-Wilson, A.L. (1992) Characterization of the major protease involved in the soybean β-conglycinin storage protein mobilization. Plant Physiology 99, 725-733.
29. Rodrigo, I., Vera, P., Van Look, L.C. and Conejero, V. (1991) Degradation of tobacco pathogenesis-related proteins. Plant Physiology 95, 616-622.
30. Rodrigo, I., Vera, P. and Conejero, V. (1989) Degradation of tomato pathogenesis-related proteins by an endogenous 37-kDa aspartyl endoproteinase. European Journal of Biochemistry 184, 663-669.
31. Runeberg-Roos, P., Kervinen, J., Kovaleva, V., Raikhel, N.V. and Gal, S. (1994) The aspartic proteinase of barley is a vacuolar enzyme that processes probarley lectin in vitro. Plant Physiology 105, 321-329.
32. Runeberg-Roos, P., Törmäkangas, K. and Östman, A. (1991) Primary structure of a barley-grain aspartic proteinase. A plant aspartic proteinase resembling mammalian cathepsin D. European Journal of Biochemistry 202, 1021-1027.
33. Schaller, A. and Ryan, C.A. (1996) Molecular cloning of a tomato leaf cDNA encoding an aspartic protease, a systemic wound response protein. Plant Molecular Biology 31, 1073-1077.
34. Shirzadegan, M., Christie, P and Seemann, J.R. (1991) An efficient method for isolation of RNA from tissue cultured plant cells. Nucleic Acids Research 19, 6055.
35. Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, A.D., Fujmato, E.K., Goeke, N.M., Olson, B.J. and Klenk, D.C. (1985) Measurement of protein using bicinchoninic acid. Analytical Biochemistry 150, 76-85.
36. Strzalka, K., Hara-Nishimura, I. and Nishimura, M, (1995) Changes in physical properties of vacuolar membrane during transformation of protein bodies in to vacuoles in germinating pumpkin seeds. Biochimica Biophysica Acta. 1239 (2), 103-110.
37. Swanson, S.J., Bethke, P.C. and Jones, R.L. (1998) Barley aleurone cells contain two types of vacuoles: characterization of lytic organelles by use of fluorescent probes. Plant Cell 10, 685-698.
38. Tökés, Z.A., Woon, W.C. and Chambers, S.M. (1974) Digestive enzymes secreted by the carnivorous plant Nepenthes macferlanei L. Planta 119, 39-46.
39. Törmäkangas, K., Kervinen, J., Östman, A. and Teeri, T. (1994) Tissue-specific localization of aspartic proteinase in developing and germinating barley grains. Planta 195, 116-125.
40. Van Der Wilden, W. and Chrispeels, M.J. (1983) Characterization of the isozymes of α-mannosidase located in the cell wall, protein bodies and endoplasmic reticulum of Phaseolus vulgaris cotyledons. Plant Physiology 71, 82-87.
41. Yatsu, L.Y. and Jacks, T.J. (1968) Association of lysosomal activity with aleurone grains in plant seeds. Archives of Biochemistry and Biophysics 124, 466-471.