Roles of LcrG and LcrV during type III targeting of effector Yops by Yersinia enterocolitica

Journal of Bacteriology

Volumn 183, Issue 15, 2001, Pages 4588-4598

  DeBord, K L ^a   Lee, V T ^a   Schneewind, O ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MEMBRANE RECEPTOR;

ARTICLE; BACTERIAL VIRULENCE; HUMAN; HUMAN CELL; KNOCKOUT GENE; PHENOTYPE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN SECRETION; PROTEIN TRANSPORT; REGULATORY MECHANISM; YERSINIA ENTEROCOLITICA;

ANTIGENS, BACTERIAL; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; CALCIUM; CYTOPLASM; HELA CELLS; HUMANS; MEMBRANE PROTEINS; MUTAGENESIS; PHENOTYPE; PORE FORMING CYTOTOXIC PROTEINS; PROTEIN-TYROSINE-PHOSPHATASE; RECOMBINANT FUSION PROTEINS; YERSINIA ENTEROCOLITICA;

EID: 0034932732     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.15.4588-4598.2001     Document Type: Article

References (66)

1. Mutational analysis of the Yersinia enterocolitica virC operon: Characterization of yscE, F, G, I, J, K required for Yop secretion and yscH encoding YopR
2. A mRNA signal for the type III secretion of Yop proteins by Yersinia enterocolitica
3. Yersinia enterocolitica type III secretion: An mRNA signal that couples translation and secretion of YopQ
4. Analysis of V antigen lcrGVH-yopBD operon of Yersinia pseudotuberculosis: Evidence for a regulatory role of lcrH and lcrV
5. Status of YopM and YopN in the Yersinia yop virulon: YopM of Y. enterocolitica is internalized inside the cytosol of PU5-1.8 macrophages by the YopB, D, N delivery apparatus
6. Yersinia enterocolitica can deliver Yop proteins into a wide range of cell types: Development of a delivery system for heterologous proteins
7. Heparin interferes with translocation of Yop proteins into HeLa cells and binds to LcrG, a regulatory component of the Yersinia Yop apparatus
8. Two independent type III secretion mechanisms for YopE in Yersinia enterolitica
9. Yersinia enterocolitica TyeA, an intracellular regulator of the type III machinery, is required for the specific targeting of YopT, YopH, YopM, and YopN into the cytosol of eukaryotic cells
10. Yersinia enterocolitica type III secretion: On the role of SycE in targeting YopE into HeLa cells
11. The virulence plasmid of Yersinia, an antihost genome
12. Restriction of DNA in Yersinia enterocolitica detected by the recipient ability for a derepressed R factor from Escherichia coli
13. The Yersinia yop virulon: A bacterial system for subverting eukaryotic cells
14. A complex composed of SycN and YscB functions as a specific chaperone for YopN in Yersinia pestis
15. trp-lac plasmids and transposons that confer conditional phenotypes
16. Virulence role of V antigen of Yersinia pestis at the bacterial surface
17. LcrV of Yersinia pestis enters infected eukaryotic cells by a virulence plasmid-independent mechanism
18. The surface-located YopN protein is involved in calcium signal transduction in Yersinia pseudotuberculosis
19. Genetic analysis of the low calcium response in Yersinia pestis Mud1(Ap lac) insertion mutants
20. The Yersinia YpkA Ser/Thr kinase is translocated and subsequently targeted to the inner surface of the HeLa cell plasma membrane
21. The YopB protein of Yersinia pseudotuberculosis is essential for the translocation of Yop effector proteins across the target cell plasma membrane and displays a contact-dependent membrane disrupting activity
22. Temperature sensing in Yersinia pestis: Translation of the LcrF activator protein is thermally regulated
23. Temperature sensing in Yersinia pestis: Regulation of yopE transcription by lcrF
24. Polymerization of a single protein of the pathogen Yersinia enterocolitica into needles punctures eukaryotic cells
25. YopK of Yersinia pseudotuberculosis controls translocation of Yop effectors across the eukaryotic cell membrane
26. Identification of SycN, YscX, and YscY, three new elements of the Yersinia yop virulon
27. YopT, a new Yersinia effector protein, affects the cytoskeleton of host cells
28. TyeA, a protein involved in control of Yop release and in translocation of Yersinia Yop effectors
29. Expression cloning of a cDNA encoding a retinoblastoma-binding protein with EF2-like properties
30. Targeting of Yersinia Yop proteins into the cytosol of HeLa cells: One-step translocation of YopE across bacterial and eukaryotic membranes is dependent on SycE chaperone
31. A program of Yersinia enterocolitica type III secretion reactions is activated by specific signals
32. Type III machines and the pathogenesis of enteric infections caused by Yersinia and Salmonella spp
33. Type III machines of pathogenic yersiniae secrete virulence factors into the extracellular milieu
34. Yersinia enterocolitica type III secretion. LcrV, a substrate for type III secretion, is required for toxin-targeting into the cytosol of HeLa cells
35. Secretion of hybrid proteins by the Yersinia Yop export system
36. Analysis of virC, an operon involved in the secretion of Yop proteins by Yersinia enterocolitica
37. Secretion of Yop proteins by yersiniae
38. Yersinia enterocolitica induces apoptosis in macrophages by a process requiring functional type III secretion and translocation mechanisms and involving YopP, presumably acting as an effector protein
39. Yersinia signals macrophages to undergo apoptosis and YopJ is necessary for this cell death
40. The V antigen of Yersinia pestis regulates Yop vectorial targeting as well as Yop secretion through effects on YopB and LcrG
41. 2+ response
42. 2+ response operon encodes the V antigen of Yersinia pestis
43. cas and FAK, and the associated accumulation of these proteins in peripheral focal adhesion
44. Cell-surface-bound Yersinia translocate the protein tyrosine phosphatase YopH by a polarized mechanism into the target cell
45. The V-antigen of Yersinia is surface exposed before target cell contact and involved in virulence protein translocation
46. Modulation of virulence factor expression by pathogen target cell contact
47. LcrD, a membrane-bound regulator of the Yersinia pestis low-calcium response
48. 2+ response virulence genes
49. Molecular analysis of lcrGVH, the V antigen operon of Yersinia pestis
50. A novel protein, LcrQ, involved in the low-calcium response of Yersinia pseudotuberculosis shows extensive homology to YopH
51. Intracellular targeting of the Yersinia YopE cytotoxin in mammalian cells induces actin microfilament disruption
52. Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells
53. The Yersinia yop virulon: LcrV is required for extrusion of the translocators YopB and YopD
54. LcrG is required for efficient translocation of Yersinia Yop effector proteins into eukaryotic cells
55. Delineation and mutational analysis of the Yersinia pseudotuberculosis YopE domains which mediate translocation across bacterial and eukaryotic cellular membranes
56. 2+ response, and virulence of Yersinia pestis
57. LcrG, a secreted protein involved in negative regulation of the low-calcium response in Yersinia pestis
58. Identification of the YopE and YopH domains required for secretion and internalization into the cytosol of macrophages, using the cyaA gene fusion approach
59. Use of T7 polymerase to direct expression of cloned genes
60. High-copy-number and low-copy-number plasmid vectors for LacZ alpha-complementation and chloramphenicol- or kanamycin-resistance selection
61. Yersinia enterocolitica type III secretlon-translocation system: Channel formation by secreted Yops
62. Individual chaperones required for Yop secretion by Yersinia
63. Identification of DNA sequences recognized by VirF, the transcriptional activator of the Yersinia yop regulon
64. YopD of Yersinia pestis plays a role in negative regulation of the low-calcium response in addition to its role in translocation of Yops
65. The cytosolic SycE and SycH chaperones of Yersinia protect the region of YopE and YopH involved in translocation across eukaryotic cell membranes
66. 2+-blind mutants of Yersinia pestis