Yersinia pestis LcrV forms a stable complex with LcrG and may have a secretion-related regulatory role in the low-Ca2+ response

Journal of Bacteriology

Volumn 179, Issue 4, 1997, Pages 1307-1316

  Nilles, Matthew L ^a   Williams, Andrew W ^a   Skrzypek, Elzbieta ^a   Straley, Susan C ^a  

^a UNIVERSITY OF KENTUCKY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL VIRULENCE; CALCIUM TRANSPORT; CROSS LINKING; IMMUNE RESPONSE; NONHUMAN; OSMOTIC STRESS; PRIORITY JOURNAL; PROTEIN PROCESSING; PROTEIN SECRETION; PROTEIN STABILITY; REPRESSOR GENE; TRANSCRIPTION REGULATION; YERSINIA PESTIS;

BACTERIA (MICROORGANISMS); EUKARYOTA; YERSINIA; YERSINIA PESTIS;

EID: 0031039346     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.4.1307-1316.1997     Document Type: Article

References (51)

1. 2+ dependency, is related to ExsB of Pseudomonas aeruginosa. J. Bacteriol. 177:4230-4237.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D.-D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1989. Current protocols in molecular biology. John Wiley & Sons, New York, N.Y.
3. Bergman, T., S. Häkansson, Å. Forsberg, L. Norlander, A. Macellaro, A. Bäckman, I. Bölin, and H. Wolf-Watz. 1991. Analysis of the V antigen IcrGVH-yopBD operon of Yersinia pseudotuberculosis: evidence for a regulatory role of LcrH and LcrV. J. Bacteriol. 173:1607-1616.
4. Birnboim, H. C., and J. Doly. 1979, A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
5. Boland, A., M.-P. Sory, M. Iriarte, C. Kerbourch, P. Wattiau, and G. R. Cornells. 1996. Status of YopM and YopN in the Yersinia Yop virulon: YopM of Y. enterocolitica is internalized inside the cytosol of PU5-1.8 macrophages by the YopB, D, N delivery apparatus. EMBO J. 15:5191-5201.
6. Brubaker, R. R., A. K. Sample, D. Z. Yu, R. J. Zachorchak, P. C. Hu, and J. M. Fowler. 1987. Proteolysis of V antigen from Yersinia pesas. Microb. Pathog. 2:49-62.
7. ++ on lysis, growth, and production of virulence antigens by Pasteurella pestis. J. Infect. Dis. 114:13-25.
8. Cornelis, G., J.-C. Vanootegem, and C. Sluiters. 1987. Transcription of the yop regulon from Y. enterocolitica requires trans acting pYV and chromosomal genes. Microb. Pathog. 2:367-379.
9. Cornelis, G. R. 1994. Yersinia pathogenicity factors. Curr. Top. Microbiol. Immunol. 192:243-263.
10. Davis, R. H., D. Botstein, and J. R. Roth. 1980. Advanced bacterial genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
11. El Yaagoubi, A., M. Kohiyama, and G. Richarme. 1994. Localization of DnaK (chaperone 70) from Escherichia coli in an osmotic-shock-sensitive compartment of the cytoplasm. J. Bacteriol. 176:7074-7078.
12. Fields, K. A., and S. C. Straley. Unpublished data.
13. Forsberg, Å., A.-M. Viitanen, M. Skurnik, and H. Wolf-Watz. 1991. The surface-located YopN protein is involved in calcium signal transduction in Yersinia pseudotuberculosis. Mol. Microbiol. 5:977-986.
14. Guzman, L.-M., D. Belin, M. J. Carson, and J. Beckwith. 1995. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177:4121-4130.
15. Häkansson, S., E. E. Galyov, R. Rosqvist, and H. Wolf-Watz. 1996. The Yersinia YpkA Ser/Thr kinase is translocated and subsequently targeted to the inner surface of the HeLa cell plasma membrane. Mol. Microbiol. 20:593-603.
16. Harlow, E., and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
17. Kado, C. I., and S. T. Liu. 1981. Rapid procedure for detection and isolation of large and small plasmids. J. Bacteriol. 145:1365-1373.
18. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
19. Lindler, L. E., M. S. Klempner, and S. C. Straley. 1990. Yersinia pestis pH 6 antigen: genetic, biochemical, and virulence characterization of a protein involved in the pathogenesis of bubonic plague. Infect. Immun. 58:2569-2577.
20. Linn, T., and R. S. Pierre. 1990. Improved vector system for constructing transcriptional fusions that ensures independent translation of lacZ. J. Bacteriol. 172:1077-1084.
21. Motin, V. I., Y. A. Nedialkov, and R. R. Brubaker. 1996. V antigen-polyhistidine fusion peptide: binding to LcrH and active immunity against plague. Infect. Immun. 64:4313-4318.
22. Mulder, B., T. Michiels, M. Simonet, M.-P. Sory, and G. Cornelis. 1989. Identification of additional virulence determinants on the pYV plasmid of Yersinia enterocolitica W227. Infect. Immun. 57:2534-2546.
23. Nakajima, R., V. L. Motin, and R. R. Brubaker. 1995. Suppression of cytokines in mice by protein A-V antigen fusion peptide and restoration of synthesis by active immunization. Infect. Immun, 63:3021-3029.
24. Nilles, M. L., and S. C. Straley. Unpublished data.
25. Nossal, N. G., and L. A. Heppel. 1966. The release of enzymes by osmotic shock from Escherichia coli in exponential phase. J. Biol. Chem. 241:3055-3062.
26. Payne, P., and S. C. Straley. Unpublished data.
27. 2+ response operon encodes the V antigen of Yersinia pestis. Infect. Immun. 54:428-434.
28. Perry, R. D., M. Pendrak, and P. Schuetze. 1990. Identification and cloning of a hemin storage locus involved in the pigmentation phenotype of Yersinia pestis. J. Bacteriol. 172:5929-5937.
29. Persson, C., R. Nordfelth, N. Holmström, S. Häkansson, R. Rosqvist, and H. Wolf-Watz. 1995. Cell-surface-bound Yersinia translocate the protein tyrosine phosphatase YopH by a polarized mechanism into the target cell. Mol. Microbiol. 18:135-150.
30. Plano, G. V., S. S. Barve, and S. C. Straley. 1991. LcrD, a membrane-bound regulator of the Yersinia pestis low-calcium response. J. Bacteriol. 173:7293-7303.
31. Piano, G. V., and S. C. Straley. 1995. Mutations in yscC, yscD, and yscG prevent high-level expression and secretion of V antigen and Yops in Yersinia pestis. J. Bacteriol. 177:3843-3854.
32. 2+ response virulence genes. J. Bacteriol. 173:2649-2657.
33. Price, S. B., K. Y. Leung, S. S. Barve, and S. C. Straley. 1989. Molecular analysis of IcrGVH, the V antigen operon of Yersinia pestis. J. Bacteriol. 171:5646-5653.
34. Price, S. B., and S. C. Straley. 1989. IcrH, a gene necessary for virulence of Yersinia pestis and for the normal response of Y. pestis to ATP and calcium. Infect. Immun. 57:1491-1498.
35. Rimpiläinen, M., Å. Forsberg, and H. Wolf-Watz. 1992. A novel protein. LcrQ, involved in the low-calcium response of Yersinia pseudotuberculosis, shows extensive homology to YopH. J. Bacteriol. 174:3355-3363.
36. Rosqvist, R., Å. Forsberg, M. Rimpiläinen, T. Bergman, and H. Wolf-Watz. 1990. The cytotoxic protein YopE of Yersinia obstructs the primary host defence. Mol. Microbiol. 4:657-667.
37. Rosqvist, R., Å. Forsberg, and H. Wolf-Watz. 1991. Intracellular targeting of the Yersinia YopE cytotoxin in mammalian cells induces actin microfilament disruption. Infect. Immun. 59:4562-4569.
38. Rosqvist, R., K.-E. Magnusson, and H. Wolf-Watz. 1994. Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells. EMBO J. 13:964-972.
39. Salmond, G. P. C., and P. J. Reeves. 1993. Membrane traffic wardens and protein secretion in Gram-negative bacteria. Trends Biochem. Sci. 18:7-12.
40. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
41. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
42. Skrzypek, E., and S. C. Straley. 1993. LcrG, a secreted protein involved in negative regulation of the low-calcium response in Yersinia pestis. J. Bacteriol. 175:3520-3528.
43. 2+ response, and virulence of Yersinia pestis. J. Bacteriol. 177:2530-2542.
44. Stark, M. J. R. 1987. Multicopy expression vectors carrying the lac represser gene for regulated high-level expression of genes in Escherichia coli. Gene. 51:255-267.
45. 2+ in Yersinia pestis include structural genes for outer membrane proteins. Infect. Immun. 51:445-454.
46. Straley, S. C., and R. R. Brubaker. 1981. Cytoplasmic and membrane proteins of yersiniae cultivated under conditions simulating mammalian intracellular environment. Proc. Natl. Acad. Sci. USA 78:1224-1228.
47. 2+ response. Mol. Microbiol. 8:1005-1010.
48. Une, T., and R. R. Brubaker. 1984. Roles of V antigen in promoting virulence and immunity in yersiniae. J. Immunol. 133:2226-2230.
49. Wattiau, P., B. Bernier, P. Deslée, T. Michiels, and G. R. Cornelis. 1994. Individual chaperones required for Yop secretion by Yersinia. Proc. Natl. Acad. Sci. USA 91:10493-10497.
50. Williams, A. W., and S. C. Straley. Unpublished data.
51. Woodcock, D. M., P. J. Crowther, J. Doherty, S. Jefferson, E. Decruz, M. Noyer-Weidner, S. Smith, M. Z. Michael, and M. W. Graham. 1989. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage rccombinants. Nucleic Acids Res. 17:3469-3478.