Modulation of virulence factor expression by pathogen target cell contact

Science

Volumn 273, Issue 5279, 1996, Pages 1231-1233

  Pettersson, Jonas ^a   Nordfelth, Roland ^a   Dubinina, Elena ^a   Bergman, Tomas ^a   Gustafsson, Mikael ^b   Magnusson, Karl Eric ^b   Wolf Watz, Hans ^a  

^a UMEÅ UNIVERSITY   (Sweden)

^b LINKÖPING UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

LUCIFERASE; VIRULENCE FACTOR;

ARTICLE; BACTERIAL VIRULENCE; CELL CONTACT; CONTROLLED STUDY; EUKARYOTIC CELL; HELA CELL; HOST PARASITE INTERACTION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; REGULATOR GENE; YERSINIA PSEUDOTUBERCULOSIS;

BACTERIA (MICROORGANISMS); EUKARYOTA; YERSINIA PSEUDOTUBERCULOSIS;

EID: 0029787714     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5279.1231     Document Type: Article

References (29)

1. G. R. Cornelis, in Society for General Microbiology Symposium 49. Molecular Biology of Bacterial Infection, C. E. Hormache, C. W. Penn., C. J. Smythe, Eds. (Cambridge Univ. Press, Cambridge, 1992), pp. 231-265.
2. Å. Forsberg, R. Rosqvist, H. Wolf-Watz, Trends Microbiol. 2, 14 (1994).
3. S. C. Straley, E. Skrzypek, G. V. Plano, J. B. Bliska, Infect. Immun. 61, 3105 (1993).
4. S. C. Straley et al., Mol. Microbiol. 8, 1005 (1993).
5. S. Håkansson, E. E. Galyov, R. Rosqvist, H. Wolf-Watz, ibid. 20, 593 (1996).
6. R. Rosqvist, K.-E. Magnusson, H. Wolf-Watz, EMBO J. 13, 964 (1994).
7. C. Persson et al., Mol. Microbiol. 18, 135 (1995).
8. M.-P. Sory and G. R. Cornelis, ibid. 14, 583 (1994).
9. M.-P, Sory, A. Boland, J. Lambermont, G. R. Cornelis, Proc. Natl. Acad. Sci. U.S.A. 92, 11998 (1995).
10. T. Michiels et al., J. Bacteriol. 173, 4994 (1991).
11. T. Bergman et al., ibid. 176, 2619 (1994).
12. R. Rosqvist, S. Håkansson, Å. Forsberg, H. Wolf-Watz, EMBO J. 14, 4187 (1995).
13. D. Hermant, R. Ménard, N. Arricau, C. Parsot, M. Y. Poppoff, Mol. Microbiol. 17, 781 (1995).
14. R. Ménard, P. Sansonetti, C. Parsot, EMBO J. 13, 5293 (1994).
15. M. Watarai, T. Tobe, M. Yoshikawa, C. Sasakawa, ibid. 14, 2461 (1995).
16. C. Ginoccio, S. B. Olmstedt, C. L. Wells, J. E. Gálan, Cell 76, 717 (1994).
17. Å. Forsberg, A.-M. Viitanen, M. Skurnik, H. Wolf-Watz, Mol. Microbiol. 5, 977 (1991).
18. O. Olsson, C. Koncz, A. Szalay, Mol. Gen. Genet. 215, 1 (1988).
19. Å. Forsberg and R. Rosqvist, Infect. Agents Dis. 2, 275 (1993).
20. M. Rimpiläinen, Å. Forsberg, H. Wolf-Watz, J. Bacteriol. 174, 3355 (1992).
21. The IcrQ mutants were constructed with a double polymerase chain reaction (PCR) strategy described in detail elsewhere (12). In short, the IcrQ gene was exchanged with the streptomycin-spectinomycin (Sp/Sm) resistance gene which was excised from mini-Tn5-Sm/Sp (26), The IcrQ deletion construct was introduced into the virulence plasmids pIB102 and pIB68 by conjugation and allelic exchange with the suicide vector pDM4 (27). The resulting virulence plasmids are denoted pIB26 (IcrQ) and pIB6826 (yscS/IcrQ), respectively. The IcrQ-IuxAB operon fusion was constructed with the same strategy as that described for the yopE-IuxAB fusion (19).
22. R. Nordfelth, unpublished results.
23. K. T. Hughes, K. L. Gillen, M. J. Semon, J. E. Karlinsey, Science 262, 1277 (1993).
24. Bacterial strains and growth conditions were as described earlier (7). Routine culturing of HeLa cells and infections were as described earlier (28).
25. Å. Forsberg, I. Bölin, L. Norlander, H. Wolf-Watz, Microb. Pathog. 2, 123 (1987).
26. V. De Lorenzo, M. Herrero, U. Jakubzik, K. Timmis, J. Bacteriol. 172, 6568 (1990).
27. D. Milton, R. O'Toole, P. Hörstedt, H. Wolf-Watz, ibid. 178, 1310 (1996).
28. R. Rosqvist and H. Wolf-Watz, Microb. Pathog. 1, 229 (1986).
29. Supported by the Swedish Medical Research Council (project number 6251 and 7490), the Swedish Natural Science Research Council, the Swedish Research Council for Engineering Sciences, the Professor Nanna Svartz Foundation, the King Gustaf the Vth 80-year Foundation, and the Swedish Society for Medical Research. J.P. was a recipient of a grant from the J. C. Kempe Memorial Foundation. E.D. was supported by the Swedish Institute.