High extracellular levels of Mycobacterium tuberculosis glutamine synthetase and superoxide dismutase in actively growing cultures are due to high expression and extracellular stability rather than to a protein-specific export mechanism

Infection and Immunity

Volumn 69, Issue 10, 2001, Pages 6348-6363

  Tullius, M V ^a   Harth, G ^a   Horwitz, M A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; GLUTAMATE AMMONIA LIGASE; GREEN FLUORESCENT PROTEIN; MALATE DEHYDROGENASE; SUPEROXIDE DISMUTASE;

ALLELISM; ARTICLE; AUTOLYSIS; BACTERIUM CULTURE; MOLECULAR CLONING; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION;

BACTERIAL PROTEINS; BIOLOGICAL TRANSPORT; CARBON; CULTURE MEDIA; CYTOCHROME B GROUP; ENZYME STABILITY; FERRITINS; GENE EXPRESSION; GLUTAMATE-AMMONIA LIGASE; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MALATE DEHYDROGENASE; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; NITROGEN; SUPEROXIDE DISMUTASE;

EID: 0034819306     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.69.10.6348-6363.2001     Document Type: Article

References (76)

1. Isolation, sequencing and expression of the superoxide dismutase-encoding gene (sod) of Nocardia asteroides strain GUH-2
2. Antigenic characterization of mycobacteria from South American wild seals
3. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
4. Effective vaccination of mice against Mycobacterium tuberculosis infection with a soluble mixture of secreted mycobacterial proteins
5. Proteins released from Mycobacterium tuberculosis during growth
6. Iron storage in bacteria
7. Antigenic equivalence of human T-cell responses to Mycobacterium tuberculosis-specific RD1-encoded protein antigens ESAT-6 and culture filtrate protein 10 and to mixtures of synthetic peptides
8. A study of mycobacterial transcriptional apparatus: Identification of novel features in promoter elements
9. Purification and properties of a unique superoxide dismutase from Nocardia asteroides
10. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels
11. Superoxide dismutases
12. Protein secretion by Gram-negative bacterial ABC exporters - A review
13. A new DNA sequence assembly program
14. Green fluorescent protein
15. Bacterial type IV secretion: Conjugation systems adapted to deliver effector molecules to host cells
16. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
17. Measurement of enzyme activities in crude extracts of tissues
18. Periplasmic superoxide dismutase protects Salmonella from products of phagocyte NADPH-oxidase and nitric oxide synthase
19. Superoxide dismutase activity of Mycobacterium avium complex (MAC) strains isolated from AIDS patients
20. Green fluorescent protein as a marker for gene expression and cell biology of mycobacterial interactions with macrophages
21. Protective role of the Mycobacterium smegmatis IdeR against reactive oxygen species and isoniazid toxicity
22. Role of Mycobacterium tuberculosis copper-zinc superoxide dismutase
23. Consensus statement. Global burden of tuberculosis: Estimated incidence, prevalence, and mortality by country. WHO Global Surveillance and Monitoring Project
24. Molecular characterization of a surface-exposed superoxide dismutase of Mycobacterium avium
25. Bacterial copper- and zinc-cofactored superoxide dismutase contributes to the pathogenesis of systemic salmonellosis
26. Common themes in microbial pathogenicity revisited
27. Fundamental aspects of reactive oxygen species, or what's the matter with oxygen?
28. Glutamine synthetase of Mycobacterium tuberculosis: Extracellular release and characterization of its enzymatic activity
29. Export of recombinant Mycobacterium tuberculosis superoxide dismutase is dependent upon both information in the protein and mycobacterial export machinery. A model for studying export of leaderless proteins by pathogenic mycobacteria
30. Expression and efficient export of enzymatically active Mycobacterium tuberculosis glutamine synthetase in Mycobacterium smegmatis and evidence that the information for export is contained within the protein
31. An inhibitor of exported Mycobactcrium tuberculosis glutamine synthetase selectively blocks the growth of pathogenic mycobacteria in axenic culture and in human monocytes: Extracellular proteins as potential novel drug targets
32. Protective immunity against tuberculosis induced by vaccination with major extracellular proteins of Mycobacterium tuberculosis
33. Color selection with a hygromycin-resistance-based Escherichia coli-mycobacterial shuttle vector
34. Type III protein secretion systems in bacterial pathogens of animals and plants
35. Molecular cloning and nucleotide sequence of the superoxide dismutase gene and characterization of its product from Bacillus subtilis
36. Comparative proteome analysis of Mycobacterium tuberculosis and Mycobacterium boris BCG strains: Towards functional genomics of microbial pathogens
37. Green fluorescent protein as a new expression marker in mycobacteria
38. Helicobacter pylori containing only cytoplasmic urease is susceptible to acid
39. Superoxide dismutase from Mycobacterium tuberculosis
40. Programmed death in bacteria
41. Measurement of cell death
42. Superoxide dismutase activity of Mycobacterium avium, M. intracellulare, and M. scrofulaceum
43. Nonpolar mutagenesis of the ipa genes defines IpaB, IpaC, and IpaD as effectors of Shigella flexneri entry into epithelial cells
44. Cloning and expression of the Mycobacterium fortuitum superoxide dismutase gene
45. Malate dehydrogenase: Distribution, function and properties
46. Isolation and partial characterization of major protein antigens in the culture fluid of Mycobacterium tuberculosis
47. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250
48. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis
49. Mycobacteria as immunogens: Development of expression vectors for use in multiple mycobacterial species
50. Immunization with extracellular proteins of Mycobacterium tuberculosis induces cell-mediated immune responses and substantial protective immunity in a guinea pig model of pulmonary tuberculosis
51. Efficient allelic exchange and transposon mutagenesis in Mycobacterium tuberculosis
52. Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis
53. The complete general secretory pathway in gram-negative bacteria
54. Nutrition, growth and metabolism
55. Extracellular enzyme activities potentially involved in the pathogenicity of Mycobacterium tuberculosis
56. Mapping and identification of Mycobacterium tuberculosis proteins by two-dimensional gel electrophoresis, microsequencing and immunodetection
57. Neutropenia restores virulence to an attenuated Cu,Zn superoxide dismutase-deficient Haemophilus ducreyi strain in the swine model of chancroid
58. The growth of Mycobacterium tuberculosis as a function of its nutrients
59. Kinetics and mechanisms of extracefiular protein release by Helicobacter pylori
60. Protein secretion in Bacillus species
61. Definition of Mycobacterium tuberculosis culture filtrate proteins by two-dimensional polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and electrospray mass spectrometry
62. Purification of Helicobacter priori superoxide dismutase and cloning and sequencing of the gene
63. The CLUSTAL X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools
64. Signal peptide-dependent protein transport in Bacillus subtilis: A genome-based survey of the secretome
65. Spectrophotometric assay for superoxide dismutase based on tetrazolium salt 3′-1-(phenylamino)-earbonyl-3,4-tetrazolium]-bis (4-methoxy-6-nitro)benzenesuulfonic acid hydrate reduction by xanthine-xanthine oxidase
66. Evidence for specific secretion rather than autolysis in the release of some Helicobacter pylori proteins
67. Electroporation at elevated temperatures substantially improves transformation efficiency of slow-growing mycobacteria
68. High resolution electroelution of polyacrylamide gels for the purification of single proteins from Mycobacterium tuberculosis culture filtrate
69. A localization index for distinction between extracellular and intracellular antigens of Mycobacterium tuberculosis
70. Cloning, expression and significance of MPT53 for identification of secreted proteins of Mycobacterium tuberculosis
71. Periplasmic superoxide dismutase in meningococcal pathogenicity
72. Regulation of glutamine synthetase. I. Purification and properties of glutamine synthetase from Escherichia coli
73. Identification and subcellular localization of a novel Cu,Zn superoxide dismutase of Mycobacterium tuberculosis
74. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors
75. Genetic analysis of superoxide dismutase, the 23 kilodalton antigen of Mycobacterium tuberculosis