Inhibition of the hepatitis C virus NS3 protease activity by Fv fragment of antibody 8D4

Biochemical and Biophysical Research Communications

Volumn 281, Issue 2, 2001, Pages 416-424

  Kasai, Nobuhiro ^a   Tsumoto, Kouhei ^a   Niwa, Shinpei ^a   Misawa, Satoru ^b   Ueno, Takamasa ^b   Hayashi, Hideya ^b   Kumagai, Izumi ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b JAPAN ENERGY CORPORATION   (Japan)

Author keywords

Antibody engineering; Fv; Hepatitis C virus; Protease inhibitor; Refolding

Indexed keywords

IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY 8D4; PROTEINASE INHIBITOR; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CYTOPLASM; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME INHIBITION; ENZYME PURIFICATION; ESCHERICHIA COLI; HEPATITIS C VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FOLDING; SEQUENCE ANALYSIS;

ESCHERICHIA COLI; HEPATITIS C VIRUS;

EID: 0034814364     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.4382     Document Type: Article

References (29)

1. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome
2. Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis
3. Diagnosis of hepatitis C virus (HCV) infection using an immunodominant chimeric polyprotein to capture circulating antibodies: Reevaluation of the role of HCV in liver disease
4. The NS3/4A proteinase of the hepatitis C virus: Unravelling structure and function of an unusual enzyme and a prime target for antiviral therapy
5. Structure and organization of the hepatitis C virus genome isolated from human carriers
6. Characterization of the hepatitis C virus-encoded serine proteinase: Determination of proteinase-dependent polyprotein cleavage sites
7. The nonstructural proteins of the hepatitis C virus: Structure and functions
8. NS3 is a serine proteinase required for processing of hepatitis C virus polyprotein
9. A central region in the hepatitis C virus NS4A protein allows formation of an active NS3-NS4A serine proteinase complex in vivo and in vitro
10. Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins
11. Recombinant antibody fragments
12. Peptide design based on an antidody complementarity-determining region (CDR): Construction of porphyrin-binding peptides and their affinity maturation by a combinatorial method
13. Synthetic peptides derived from the variable regions of an anti-CD4 monoclonal antibody bind to CD4 and inhibit HIV-1 promoter activation in virus-infected cells
14. Peptides derived from the complementarity-determining regions of anti-Mac-1 antibodies block intercellular adhesion molecule-1 interaction with Mac-1
15. Isolation and characterization of monoclonal antibodies that inhibit hepatitis C virus NS3 protease
16. Novel selection method for engineered antibodies using the mechanism of Fv fragment stabilization in the presence of antigen
17. Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent - Application to a human single-chain Fv fragment
18. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase
19. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
20. IMGT, the International ImMunoGeneTics database
21. Folding and assembly of an antibody Fv fragment, a heterodimer stabilized by antigen
22. Hepatitis C virus NS3/4A protease
23. The crystal structure of hepatitis C virus NS3 proteinase reveals a trypsin-like fold and a structural zinc binding site
24. The solution structure of the N-terminal proteinase domain of the hepatitis C virus (HCV) NS3 protein provides new insights into its activation and catalytic mechanism
25. Thermodynamic consequences of grafting enhanced affinity toward the mutated antigen onto an antibody. The case of anti-lysozyme antibody, HyHEL-10
26. Selection of phage antibodies by binding affinity. Mimicking affinity maturation
27. Isolation of picomolar affinity anti-c-erbB-2 single-chain Fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site
28. Affinity maturation of a high-affinity human monoclonal antibody against the third hypervariable loop of human immunodeficiency virus: Use of phage display to improve affinity and broaden strain reactivity