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Volumn 14, Issue 8, 2001, Pages 573-582
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Design, high-level expression, purification and characterization of soluble fragments of the hepatitis C virus NS3 RNA helicase suitable for NMR-based drug discovery methods and mechanistic studies
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Author keywords
HCV NS3 RNA helicase; NMR based screening; Protein engineering; Protein NMR; Recombinant expression
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Indexed keywords
ADENOSINE 5' O (3 THIOTRIPHOSPHATE);
AMINO ACID;
DOUBLE STRANDED RNA;
NITROGEN 15;
NUCLEIC ACID;
NUCLEOSIDE TRIPHOSPHATASE;
NUCLEOSIDE TRIPHOSPHATE;
RNA HELICASE;
SERINE PROTEINASE;
VIRUS RNA;
ARTICLE;
BINDING SITE;
DNA SEQUENCE;
ENZYME ACTIVITY;
ENZYME ANALYSIS;
ENZYME PURIFICATION;
GENETIC ENGINEERING;
HEPATITIS C VIRUS;
NONHUMAN;
NUCLEAR MAGNETIC RESONANCE;
POLYACRYLAMIDE GEL ELECTROPHORESIS;
PRIORITY JOURNAL;
PROTEIN EXPRESSION;
STEADY STATE;
ADENOSINE TRIPHOSPHATASES;
AMINO ACID SEQUENCE;
ANTIVIRAL AGENTS;
DRUG DESIGN;
KINETICS;
MOLECULAR SEQUENCE DATA;
NITROGEN ISOTOPES;
NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR;
PEPTIDE FRAGMENTS;
PROTEIN ENGINEERING;
PROTEIN STRUCTURE, TERTIARY;
SOLUBILITY;
TRANSDUCTION, GENETIC;
VIRAL NONSTRUCTURAL PROTEINS;
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EID: 0034788713
PISSN: 02692139
EISSN: None
Source Type: Journal
DOI: 10.1093/protein/14.8.573 Document Type: Article |
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Times cited : (10)
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References (65)
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