Further characterization of the sixth transmembrane domain of Pgp1 by site-directed mutagenesis

Cancer Chemotherapy and Pharmacology

Volumn 48, Issue 5, 2001, Pages 339-346

  Song, J ^a   Melera, P W ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

MDR; P glycoprotein; Site directed mutagenesis; TM6

Indexed keywords

ABC TRANSPORTER; AZIDOPRAZOSIN; COLCHICINE; COMPLEMENTARY DNA; CYCLOSPORIN A; DACTINOMYCIN; DAUNORUBICIN; GLYCOPROTEIN P; PRAZOSIN; PROLINE; UNCLASSIFIED DRUG; VERAPAMIL;

ALPHA HELIX; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DRUG SENSITIVITY; ENZYME ACTIVATION; ENZYME ACTIVITY; GENETIC TRANSFECTION; HOST CELL; HYDROPHILICITY; HYDROPHOBICITY; MULTIDRUG RESISTANCE; NONHUMAN; PHOTOAFFINITY LABELING; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN; SITE DIRECTED MUTAGENESIS;

ADENOSINE TRIPHOSPHATASES; ANIMALS; AZIDES; CELL LINE; CRICETINAE; HYDROPHOBICITY; MEMBRANE PROTEINS; MUTAGENESIS, SITE-DIRECTED; P-GLYCOPROTEIN; PHOTOAFFINITY LABELS; PRAZOSIN; STRUCTURE-ACTIVITY RELATIONSHIP; TRANSFECTION; VERAPAMIL;

EID: 0034765599     PISSN: 03445704     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002800100354     Document Type: Article

References (29)

1. Characteristics of multidrug resistance in human tumor cells
2. Characterization of the azidopine and vinblastine binding site of P-glycoprotein
3. Multidrug-resistant human sarcoma cells with a mutant P-glycoprotein altered phenotype and resistance to cyclosporins
4. Iodomycin and iodipine, a structural analogue of azidopine, bind to a common domain in hamster P-glycoprotein
5. Functional studies with a full length P-glycoprotein cDNA encoded by the Hamster pgp1 gene
6. Diversity of multidrug resistance in mammalian cells
7. Amino acid substitutions in the sixth transmembrane domain of P-glycoprotein alter multidrug resistance
8. Evidence for two nonidentical drug-interaction sites in the human P-glycoprotein
9. The biochemistry of P-glycoprotein-mediated multidrug resistance
10. P-glycoprotein - A mediator of multidrug resistance in tumor cells
11. Major photoaffinity drug labeling sites for iodoaryl azidoprazosin in P-glycoprotein are within, or immediately C-terminal to, transmembrane domains 6 and 12
12. Contribution to substrate specificity and transport of nonconserved residues in transmembrane domain 12 of human P-glycoprotein
13. Mutagenesis of transmembrane domain 11 of P-glycoprotein by alanine scanning
14. ABC transporters: From microorganisms to man
15. Structural flexibility of the linker region of human P-glycoprotein permits ATP hydrolysis and drug transport
16. Functional consequences of phenylalanine mutations in the predicted transmembrane domain of P-glycoprotein
17. Mutations to amino acids located in predicted transmembrane segment 6 (TM6) modulate the activity and substrate specificity of human P-glycoprotein
18. Identification of residues in the drug-binding domain of human P-glycoprotein
19. The packing of the transmembrane segments of human multidrug resistance P-glycoprotein is revealed by disulfide cross-linking analysis
20. Mutations in the sixth transmembrane domain of P-glycoprotein that alter the pattern of cross-resistance also alter sensitivity to cyclosporin A reversal
21. P-glycoprotein function involves conformational transitions detected by differential immunoreactivity
22. Human P-glycoprotein exhibits reduced affinity for substrates during a catalytic transition state
23. Expression of the human multidrug resistance cDNA in insect cells generates a high activity drug-stimulated membrane ATPase
24. Evidence for a requirement for ATP hydrolysis at two distinct steps during a single turnover of the catalytic cycle of human P-glycoprotein
25. Multi-ion pore behavior in the CFTR chloride channel
26. Alteration of substrate specificity by mutations at the His61 position in predicted transmembrane domain 1 of human MDR/P-glycoprotein
27. Conformational changes of p-glycoprotein by nucleotide binding
28. Involvement of cytoplasmic factors regulating the membrane orientation of P-glycoprotein sequences