Alteration of Cre recombinase site specificity by substrate-linked protein evolution

Nature Biotechnology

Volumn 19, Issue 11, 2001, Pages 1047-1052

  Buchholz, Frank ^a   Stewart, A Francis ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLIFICATION; DNA; GENES; MOLECULES;

MULTICYCLE TASK;

PROTEINS;

DNA; RECOMBINASE;

ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; CHO CELL; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; DNA RECOMBINATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE TARGETING; GENETIC ENGINEERING; LONG TERMINAL REPEAT; MOLECULAR EVOLUTION; MOLECULAR RECOGNITION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

3T3 CELLS; ANIMALS; BASE SEQUENCE; BINDING SITES; CHO CELLS; CRICETINAE; DIRECTED MOLECULAR EVOLUTION; DNA MUTATIONAL ANALYSIS; DNA-BINDING PROTEINS; ESCHERICHIA COLI; HUMANS; INTEGRASES; MICE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; PROTEIN STRUCTURE, SECONDARY; RECOMBINATION, GENETIC; SUBSTRATE SPECIFICITY; VIRAL PROTEINS;

EID: 0034754491     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt1101-1047     Document Type: Article

References (46)

1. In vitro selection and evolution of proteins
2. Directed evolution of biocatalysts
3. In vitro selection of functional nucleic acids
4. Directed evolution of an RNA enzyme
5. Totally in vitro protein selection using mRNA-protein fusions and ribosome display
6. Recent advances in producing and selecting functional proteins by using cell-free translation
7. Man-made cell-like compartments for molecular evolution
8. Improved properties of FLP recombinase evolved by cycling mutagenesis
9. DNA specificity of the Cre recombinase resides in the 25 kDa carboxyl domain of the protein
10. Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse
11. Mechanism of strand cleavage and exchange in the Cre-lox site-specific recombination system
12. The role of the loxP spacer region in P1 site-specific recombination
13. High-efficiency deleter mice show that FLPe is an alternative to Cre-loxP
14. Widespread recombinase expression using FLPeR Flipper) mice
15. Mutation rates among RNA viruses
16. Identifying determinants of recombination specificity: Construction and characterization of mutant bacteriophage integrases
17. Identifying determinants of recombination specificity: Construction and characterization of chimeric bacteriophage integrases
18. The right time and place for molecular scissors
19. Engineering the mouse genome by site-specific recombination
20. Cre recombinase: The universal reagent for genome tailoring
21. DNA cassette exchange in ES cells mediated by Flp recombinase: An efficient strategy for repeated modification of tagged loci by marker-free constructs
22. Segmental genomic replacement in embryonic stem cells by double lox targeting
23. Role of nucleotide sequences of IoxP spacer region in Cre-mediated recombination
24. Site-specific chromosomal integration in mammalian cells: Highly efficient CRE recombinase-mediated cassette exchange
25. Characterization of Cre-mediated cassette exchange after plasmid microinjection in fertilized mouse oocytes
26. Mammalian genomes contain active recombinase recognition sites
27. Site-specific genomic integration in mammalian cells mediated by phage phiC 31 integrase
28. Group II introns designed to insert into therapeutically relevant DNA target sites in human cells
29. Site-specific integration of an adeno-associated virus vector plasmid mediated by regulated expression of rep based on Cre-loxP recombination
30. Ligand-regulated site-specific recombination
31. Genetics of retroviral integration
32. Self-deleting retrovirus vectors for gene therapy
33. Transfer of single gene-containing long terminal repeats into the genome of mammalian cells by a retroviral vector carrying the cregene and the loxP site
34. Reversible immortalization of human primary cells by lentivector-mediated transfer of specific genes
35. Development of an HIV-1-dependent expression vector with the Cre/loxP system
36. Inhibition of recombinant human immunodeficiency virus type 1 replication by a site-specific recombinase
37. A novel mutant loxP containing part of long terminal repeat of HIV-1 in spacer region: Presentation of possible target site for antiviral strategy using site-specific recombinase
38. Characterization of Cre-loxP interaction in the major groove: Hint for structural distortion of mutant Cre and possible strategy for HIV- 1 therapy
39. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
40. Rapid evolution of a protein in vitro by DNA shuffling
41. Molecular evolution by staggered extension process (STEP) in vitro recombination
42. Different thermostabilities of FLP and Cre recombinases: Implications for applied site-specific recombination
43. Inducible site-directed recombination in mouse embryonic stem cells
44. Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination
45. Asymmetric DNA bending in the Cre-loxP site-specific recombination synapse
46. Regulation of Cre recombinase activity by the synthetic steroid RU 486