Expression of sterol 12α-hydroxylase alters bile acid pool composition in primary rat hepatocytes and in vivo

Gastroenterology

Volumn 120, Issue 7, 2001, Pages 1801-1809

  Pandak, William M ^a   Bohdan, Patricia ^a   Franklund, Clifton ^c   Mallonee, Darrell H ^c   Eggertsen, Gösta ^d   Björkhem, Ingemar ^d   Gil, Gregorio ^b   Vlahcevic, Z Reno ^a,c   Hylemon, Phillip B ^c  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

^b Department of Biochemistry and Molecular Biophysics ^*  (United States)

^c VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BILE ACID; CHENODEOXYCHOLIC ACID; CHOLESTEROL 7ALPHA MONOOXYGENASE; CHOLIC ACID; STEROL 12ALPHA HYDROXYLASE; STEROL DERIVATIVE; UNCLASSIFIED DRUG; CYTOCHROME P450; STEROID 12ALPHA MONOOXYGENASE; STEROID MONOOXYGENASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BILE ACID SYNTHESIS; CHEMICAL COMPOSITION; CONTROLLED STUDY; HYDROPHOBICITY; HYDROXYLATION; IN VIVO STUDY; LIVER CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; ANIMAL; BIOSYNTHESIS; CELL CULTURE; GENETICS; MALE; METABOLISM; PHYSIOLOGY; SPRAGUE DAWLEY RAT;

ANIMALS; CELLS, CULTURED; CHENODEOXYCHOLIC ACID; CHOLESTEROL 7-ALPHA-HYDROXYLASE; CHOLIC ACID; CYTOCHROME P-450 ENZYME SYSTEM; HEPATOCYTES; MALE; RATS; RATS, SPRAGUE-DAWLEY; STEROID 12-ALPHA-HYDROXYLASE; STEROID HYDROXYLASES;

EID: 0034743930     PISSN: 00165085     EISSN: None     Source Type: Journal    
DOI: 10.1053/gast.2001.24833     Document Type: Article

References (39)

1. Regulation of bile acid biosynthesis
2. Regulation of bile acid synthesis: III. Correlation between biliary bile salt hydrophobicity index and the activities of enzyme regulating cholesterol and bile acid synthesis in the rat
3. Regulation of cholesterol 7α-hydroxylase mRNA and transcriptional activity by taurocholate and cholesterol in the chronic biliary diverted rat
4. Transcriptional regulation of cholesterol 7α-hydroxylase mRNA by conjugated bile acids in primary cultures of rat hepatocytes
5. Bile acid biosynthesis
6. Transcriptional regulation of hepatic sterol 27-hydroxylase by bile acids
7. An in vivo evaluation of quantitative significance of several potential pathways to cholic and chenodeoxycholic acids from cholesterol in man
8. 27-Hydroxycholesterol: Production rates in normal human subjects
9. Potential bile acid precursors in plasma: Possible indicators of biosynthetic pathways of cholic and chenodeoxycholic acids in man
10. Quantitative estimations of the contribution of different bile acid pathways to total bile acid synthesis in the rat
11. Cyclosporin A blocks bile acid synthesis in cultured hepatocytes by specific inhibition of chenodeoxycholic acid synthesis
12. Regulation of sterol 27-hydroxylase and an alternative pathway of bile acid biosynthesis in primary cultures of rat hepatocytes
13. Oxysterol 7α-hydroxylase activity by cholesterol 7α-hydroxylase
14. Regulation of sterol 12α-hydroxylase and cholic acid biosynthesis in the rat
15. Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity, protein mass and mRNA levels by hydrophobic bile acids in the rat
16. Effects of four taurine-conjugated bile acids on mucosal uptake and lymphatic absorption of cholesterol in the rat
17. Regulation of multidrug resistance 2 P-glycoprotein expression by bile salts in rats and in primary cultures of rat hepatocytes
18. Enterohepatic circulation
19. Physiology and pathophysiology of enterohepatic circulation of bile acids
20. Pathogenesis and dissolution of gallstones
21. Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells
22. Viral vectors for gene delivery
23. Regulation of cholesterol 7α-hydroxylase in the liver
24. Characteristics of a human cell line transformed by human adenovirus type 5
25. Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli
26. Improved radiosensitization of rat glioma cells with adenovirus-expressed mutant HSV-TK in combination with acyclovir
27. Phenotypic stability of adult rat hepatocytes in primary monolayer culture
28. A simple method for the isolation and purification of total lipids from animal tissues
29. Hepatocellular protein kinase C activation by bile acids: Implications for regulation of cholesterol 7α-hydroxylase
30. Adenovirus-mediated transfer of a gene encoding cholesterol 7α-hydroxylase into hamsters increases hepatic enzyme activity and reduces plasma total and low density lipoprotein cholesterol
31. Overexpression of cholesterol 7α-hydroxylase (CYP7a) in mice lacking the low density lipoprotein (LDL) receptor gene
32. Endogenous bile acids are ligands for the nuclear receptor FXR/BAR
33. Identification of a nuclear receptor for bile acids
34. Bile acids: Natural ligands for an orphan nuclear receptor
35. Activation of protein kinase Cα and δ by bile acids: Correlation with bile acid structure and diacylglycerol formation
36. Insulin is a dominant suppressor of sterol 12 alpha-hydroxylase P450 (CYP8b) expression in rat liver: Possible role of insulin in circadian rhythm of CYP8B
37. Alpha 1-fetoprotein transcription factor is required for the expression of sterol 12 alpha-hydroxylase, the specific enzyme for cholic acid synthesis. Potential role in the bile acid-mediated regulation of gene transcription
38. Mechanism of bile acid biosynthesis in mammalian liver
39. Hormonal regulation of cholesterol 7α-hydroxylase mRNA levels and transcriptional activity in primary rat hepatocytes cultures