Bile acids: Natural ligands for an orphan nuclear receptor

Science

Volumn 284, Issue 5418, 1999, Pages 1365-1368

  Parks, Derek J ^a   Blanchard, Steven G ^a   Bledsoe, Randy K ^a   Chandra, Gyan ^a   Consler, Thomas G ^a   Kliewer, Steven A ^a   Stimmel, Julie B ^a   Willson, Timothy M ^a   Zavacki, Ann Marie ^b   Moore, David D ^b   Lehmann, Jürgen M ^a  

^a GLAXOSMITHKLINE   (United States)

^b BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BILE ACID; CELL NUCLEUS RECEPTOR;

ANIMAL CELL; ARTICLE; CHEMICAL STRUCTURE; CONTROLLED STUDY; GENETIC TRANSFECTION; HOMEOSTASIS; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN PROTEIN INTERACTION; STRUCTURE ACTIVITY RELATION;

ANIMALS; BILE ACIDS AND SALTS; CARRIER PROTEINS; CELL LINE; CHENODEOXYCHOLIC ACID; CHOLESTEROL; DEOXYCHOLIC ACID; DNA-BINDING PROTEINS; HISTONE ACETYLTRANSFERASES; HOMEOSTASIS; HUMANS; LIGANDS; LITHOCHOLIC ACID; MICE; ORGANIC ANION TRANSPORTERS, SODIUM-DEPENDENT; PROTEIN CONFORMATION; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION; STRUCTURE-ACTIVITY RELATIONSHIP; SYMPORTERS; TRANSCRIPTION FACTORS; TRANSFECTION;

ANIMALIA;

EID: 0033591387     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.284.5418.1365     Document Type: Article

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31. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N. Asn; P. Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
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35. 2] (27) (SynPEP) that was labeled with streptavidin-conjugated europium chelate (Wallac), in 50 mM tris pH 8, 50 mM KCl, 0.1 mg/ml bovine serum albumin, 1 mM EDTA, and 10 mM dithiothreitol, in the presence of test compound for 2 hours at 22°C. Data were collected with a Wallac Victor fluorescence reader in a time-resolved mode. The relative fluorescence was measured at 665 nM, and the indicated values were calculated by subtracting the fluorescence obtained in the absence of test compound from the value obtained in the presence of test compound. Values are expressed as the means ± SD derived from three independent experiments.
36. We thank P. A. Dawson for providing the IBAT expression plasmid pCMV-HISBT, B. W. O'Malley, S. Y. Tsai, M.-Y. Tsai, and M. C. Lewis for critically reading the manuscript. Supported in part by NIH grant RO1 DK53366 to D.D.M. and National Institute of Diabetes and Digestive and Kidney Diseases grant F32 DK09793 to A.M.Z.