Site-directed mutagenesis of human Lysyl hydroxylase expressed in insect cells: Identification of histidine residues and an aspartic acid residue critical for catalytic activity

Journal of Biological Chemistry

Volumn 271, Issue 16, 1996, Pages 9398-9402

  Pirskanen, Asta ^a   Kaimio, Anne Maarit ^a   Myllylä, Raili ^a   Kivirikko, Kari I ^a  

^a UNIVERSITY OF OULU   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

INSECTA; UNIDENTIFIED BACULOVIRUS;

ASPARTIC ACID; HISTIDINE; PROCOLLAGEN LYSINE 2 OXOGLUTARATE 5 DIOXYGENASE; VIRUS VECTOR;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE; GENE EXPRESSION; GLYCOSYLATION; INSECT; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANIMALS; ASPARAGINE; ASPARTIC ACID; BACULOVIRIDAE; BASE SEQUENCE; BINDING SITES; CELL LINE; CODON; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENETIC VECTORS; HISTIDINE; HUMANS; KINETICS; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROCOLLAGEN-LYSINE, 2-OXOGLUTARATE 5-DIOXYGENASE; RECOMBINANT PROTEINS; SPODOPTERA; TRANSFECTION;

EID: 0029887663     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.271.16.9398     Document Type: Article

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