Analysis of the interactions of human ribonuclease inhibitor with angiogenin and ribonuclease A by mutagenesis: Importance of inhibitor residues inside versus outside the C-terminal 'hot spot'

Journal of Molecular Biology

Volumn 302, Issue 2, 2000, Pages 497-519

  Shapiro, Robert ^a   Ruiz Gutierrez, Melisa ^a   Chen, Chang Zheng ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Angiogenin; Binding energy; Protein protein interactions; Ribonuclease inhibitor; Site directed mutagenesis

Indexed keywords

ANGIOGENIN; RIBONUCLEASE; RIBONUCLEASE A;

ARTICLE; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CRYSTAL STRUCTURE; ENERGY TRANSFER; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME KINETICS; HUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS;

EID: 0034665638     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4075     Document Type: Article

References (54)

1. Structural plasticity in a remodeled protein-protein interface
2. Role of the N terminus in RNase A homologues: Differences in catalytic activity, ribonuclease inhibitor interaction and cytotoxicity
3. Structure of an anti-body-lysozyme complex unexpected effect of conservative mutation
4. Site-specific mutagenesis reveals differences in the structural bases for tight binding of RNase inhibitor to angiogenin and RNase A
5. Superadditive and sub-additive effects of 'hot spot' mutations within the interfaces of placental ribonuclease inhibitor with angiogenin and ribonuclease A
6. Hydrophobic bonding and accessible surface area in proteins
7. A hot spot of binding energy in a hormone-receptor interface
8. Structural and functional analysis of the 1:1 growth hormone:receptor complex reveals the molecular basis for receptor affinity
9. Analysis of protein-protein interactions and the effects of amino acid mutations on their energetics. The importance of water molecules in the binding epitope
10. A mutational analysis of the binding of two different proteins to the same anti-body
11. An extensively modified version of MolScript that includes greatly enhanced coloring capabilities
12. Hydrogen bonding and biological specificity analysed by protein engineering
13. Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells
14. Analysis of binding interactions in an idiotope-antiidiotope protein-protein complex by double mutant cycles
15. Site-directed mutagenesis by overlap extension using the polymerase chain reaction
16. Ribonuclease inhibitor
17. Purification and characterization of truncated ribonuclease inhibitor
18. Dissecting the energetics of an antibody-antigen interface by alanine shaving and molecular grafting
19. Hyper-sensitive substrate for ribonucleases
20. Thermodynamic analysis of an antibody functional epitope
21. A structural basis of the interactions between leucine-rich repeats and protein ligands
22. Mechanism of ribonuclease inhibition by ribonuclease inhibitor protein based on the crystal structure of its complex with ribonuclease A
23. MOLSCRIPT - A program to produce both detailed and schematic plots of protein structures
24. Binding of placental ribonuclease inhibitor to the active site of angiogenin
25. Expression of human placental ribonuclease inhibitor in Escherichia coli
26. Kinetic characterization of two active mutants of placental ribonuclease inhibitor that lack internal repeats
27. Primary structure of human placental ribonuclease inhibitor
28. Tryptophan fluorescence as a probe of placental ribonuclease inhibitor binding to angiogenin
29. Tight-binding inhibition of angiogenin and ribonuclease A by placental ribonuclease inhibitor
30. Ribonuclease A variants with potent cytotoxic activity
31. Refined crystal structures of native human angiogenin and two active site variants: Implications for the unique functional properties of an enzyme involved in neovascularisation during tumour growth
32. 15N-NMR spectroscopy-characterization of histidine protonation states and pKa values
33. Long-range, small magnitude nonadditivity of mutational effects in proteins
34. The atomic structure of protein-protein recognition sites
35. Kinetics of the reversible inhibition of enzyme-catalysed reactions by tight-binding inhibitors
36. VEGF and the Fab fragment of a humanized neutralizing antibody: Crystal structure of the complex at 2.4 Å resolution and mutational analysis of the interface
37. Entropy in biological binding processes: Estimation of translational entropy loss
38. Interaction of semisynthetic variants of RNase A with ribonuclease inhibitor
39. Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons
40. Empirical free energy calculations: A blind test and further improvements to the method
41. Analysis of protein-protein interactions by mutagenesis: Direct versus indirect effects
42. Molecular recognition of human angiogenin by placental ribonuclease inhibitor: An X-ray crystallographic study at 2.0 Å resolution
43. Energetics of protein-protein interactions: Analysis of the barnase-barstar interface by single mutations and double mutant cycles
44. The role of Glu73 of barnase in catalysis and the binding of barstar
45. Site-directed mutagenesis of histidine-13 and histidine-114 of human angiogenin. Alanine derivatives inhibit angiogenin-induced angiogenesis
46. Interaction of human placental ribonuclease with placental ribonuclease inhibitor
47. Identification of functional arginines in human angiogenin by site-directed mutagenesis
48. Characteristic ribonucleolytic activity of human angiogenin
49. Engineering receptor-mediated cytotoxicity into human ribonucleases by steric blockade of inhibitor interaction
50. Structural response to mutation at a protein-protein interface
51. Protein chemical and kinetic characterization of recombinant porcine ribonuclease inhibitor expressed in Saccharomyces cerevisiae
52. Mechanism and binding sites in the ribonuclease reaction II. Kinetic studies on the first step of the reaction
53. Solvent rearrangement in an antigen-antibody interface introduced by site-directed mutagenesis of the antibody combining site
54. A novel fluorogenic substrate for ribonucleases. Synthesis and enzymatic characterization