Engineering receptor-mediated cytotoxicity into human ribonucleases by steric blockade of inhibitor interaction

Nature Biotechnology

Volumn 17, Issue 3, 1999, Pages 265-270

  Suzuki, Motoshi ^a   Saxena, Shailendra K ^a,b   Boix, Ester ^a,c   Prill, Robert J ^a   Vasandani, Veena M ^a,d   Ladner, Jane E ^e   Sung, Cynthia ^f   Youle, Richard J ^a  

^a NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE   (United States)

^b Alfacell Corporation   (United States)

^c University of Bath   (United Kingdom)

^d UNIVERSITY OF VIRGINIA   (United States)

^e UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^f NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Eosinophil derived neurotoxin; Immunotoxin; Receptor mediated endocytosis; Ribonuclease inhibitor; Site directed mutagenesis

Indexed keywords

RIBONUCLEASE A; SULFIDE; TRANSFERRIN; TRANSFERRIN RECEPTOR;

ANTINEOPLASTIC ACTIVITY; ARTICLE; CYTOTOXICITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INHIBITION; GENETIC LINKAGE; PRIORITY JOURNAL; PROTEIN BINDING; RECEPTOR AFFINITY; RECEPTOR BINDING; RECEPTOR BLOCKING; STEREOCHEMISTRY; STEREOSPECIFICITY; STRUCTURE ACTIVITY RELATION;

ANIMALS; CRYSTALLOGRAPHY, X-RAY; DOSE-RESPONSE RELATIONSHIP, DRUG; EOSINOPHIL-DERIVED NEUROTOXIN; GLIOMA; HUMANS; INHIBITORY CONCENTRATION 50; KINETICS; LEUCINE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; PROTEIN ENGINEERING; PROTEINS; RECEPTORS, TRANSFERRIN; RECOMBINANT PROTEINS; RIBONUCLEASE, PANCREATIC; RIBONUCLEASES; SWINE; TRANSFERRIN; TUMOR CELLS, CULTURED;

EID: 0032996760     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/7010     Document Type: Article

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