Rapid microplate assay for superoxide scavenging efficiency

Journal of Neuroscience Methods

Volumn 97, Issue 2, 2000, Pages 139-144

  Quick, Kevin L ^a   Hardt, Joshua I ^a   Dugan, Laura L ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Catalase; Cell culture; Cytochrome c reduction; Hypoxanthine; Oxidation reduction; Superoxide radical; Xanthine oxidase

Indexed keywords

CATALASE; CYTOCHROME C; EDETIC ACID; HYPOXANTHINE; SUPEROXIDE; XANTHINE OXIDASE;

ANIMAL CELL; ARTICLE; BRAIN CORTEX; CELL LINE; CONTROLLED STUDY; DRUG SCREENING; HYPOTHALAMUS; IN VITRO STUDY; MOUSE; NERVE CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; SCAVENGING SYSTEM; SPECTROPHOTOMETRY; TECHNIQUE;

ANIMALS; CATALASE; CELLS, CULTURED; CYTOCHROME C GROUP; FREE RADICAL SCAVENGERS; HYPOXANTHINE; MICE; MICROCHEMISTRY; NEOCORTEX; NEURONS; OXIDATION-REDUCTION; SUPEROXIDE DISMUTASE; SUPEROXIDES; XANTHINE OXIDASE;

EID: 0034655723     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(00)00179-5     Document Type: Article

References (31)

1. Bay B.-H., Lee Y.-K., Tan B., Ling E.-A. Lipid peroxidative stress and antioxidant enzymes in brains of milk-supplemented rats. Neurosci. Lett. 277:1999;127-130.
2. Beal M.F. Mitochondrial dysfunction in neurodegenerative diseases. Biochim. Biophys. Acta. 1366:1998;211-223.
3. Bloor J.H., Holtz D., Kaars J., Kosman D.J. Characterization of superoxide dismutase (SOD-1 and SOD-2) activities in inbred mice: evidence for quantitative variability and possible nonallelic SOD-1 polymorphism. Biochem. Genet. 21:1983;349-364.
4. Browne S.E., Ferrante R.J., Beal M.F. Oxidative stress in Huntington's disease. Brain Pathol. 9:1999;147-163.
5. Cassarino D.S., Bennett J.P. Jr An evaluation of the role of mitochondria in neurodegenerative diseases: mitochondrial mutations and oxidative pathology, protective nuclear responses, and cell death in neurodegeneration. Brain Res. Brain Res. Rev. 29:1999;1-25.
6. Castagne V., Gautschi M., Lefevre K., Posada A., Clarke P.G. Relationships between neuronal death and the cellular redox status. Focus on the developing nervous system. Prog. Neurobiol. 59:1999;397-423.
7. Crapo J.D., McCord J.M., Fridovich I. Preparation and assay of superoxide dismutases. Methods Enzymol. 53:1978;382-393.
8. Dugan L.L., Bruno V.M.G., Amagasu S.M., Giffard R.G. Glia modulate the response of murine cortical neurons to excitotoxicity: glia exacerbate AMPA neurotoxicity. J. Neurosci. 15:1995;4545-4555.
9. Finkel T. Signal transduction by reactive oxygen species in non-phagocytic cells. J. Leukocyte Biol. 65:1999;337-340.
10. Fridovich I. Quantitative aspects of the production of superoxide anion radical by milk xanthine oxidase. J. Biol. Chem. 245:1970;4053-4057.
11. Fujita K., Yamauchi M., Shibayama K., Ando M., Honda M., Nagata Y. Decreased cytochrome c oxidase activity but unchanged superoxide dismutase and glutathione peroxidase activities in the spinal cords of patients with amyotrophic lateral sclerosis. J. Neurosci. Res. 45:1996;276-281.
12. Greenlund L.J., Deckwerth T.L., Johnson E.M. Jr Superoxide dismutase delays neuronal apoptosis: a role for reactive oxygen species in programmed neuronal death. Neuron. 14:1995;303-315.
13. Halliwell B. Antioxidants in human health and disease. Annu. Rev. Nutr. 16:1996;33-50.
14. Hogg N. Free radicals in disease. Semin. Reprod. Endocrinol. 16:1998;241-248.
15. Huang W.C., Juang S.W., Liu I.M., Chi T.C., Cheng J.T. Changes of superoxide dismutase gene expression and activity in the brain of streptozotocin-induced diabetic rats. Neurosci. Lett. 275:1999;25-28.
16. Kamata H., Hirata H. Redox regulation of cellular signalling. Cell Signal. 11:1999;1-14.
17. Kawase M., Murakami K., Fujimura M., Morita-Fujimura Y., Gasche Y., Kondo T., Scott R.W., Chan P.H. Exacerbation of delayed cell injury after transient global ischemia in mutant mice with CuZn superoxide dismutase deficiency. Stroke. 30:1999;1962-1968.
18. Kinouchi H., Kamii H., Mikawa S., Epstein C.J., Yoshimoto T., Chan P.H. Role of superoxide dismutase in ischemic brain injury: a study using SOD-1 transgenic mice. Cell. Mol. Neurobiol. 18:1998;609-620.
19. Kondo T., Reaume A.G., Huang T.T., Murakami K., Carlson E., Chen S., Scott R.W., Epstein C.J., Chan P.H. Edema formation exacerbates neurological and histological outcomes after focal cerebral ischemia in CuZn-superoxide dismutase gene knockout mutant mice. Acta Neurochir. Suppl. (Wien). 70:1997;62-64.
20. Li Y., Huang T.T., Carlson E.J., Melov S., Ursell P.C., Olson J.L., Noble L.J., Yoshimura M.P., Berger C., Chan P.H., Wallace D., Epstein C. Dilated cardiomyopathy and neonatal lethality in mutant mice lacking manganese superoxide dismutase. Nat. Genet. 11:1995;376-381.
21. Mancini M., Anderson B.O., Caldwell E., Sedghinasab M., Paty P.B., Hockenbery D.M. Mitochondrial proliferation and paradoxical membrane depolarization during terminal differentiation and apoptosis in a human colon carcinoma cell line. J. Cell Biol. 138:1997;449-469.
22. Murakami K., Kondo T., Kawase M., Li Y., Sato S., Chen S.F., Chan P.H. Mitochondrial susceptibility to oxidative stress exacerbates cerebral infarction that follows permanent focal cerebral ischemia in mutant mice with manganese superoxide dismutase deficiency. J. Neurosci. 18:1998;205-213.
23. Olson J.S., Ballou D.P., Palmer G., Massey V. The mechanism of action of xanthine oxidase. J. Biol. Chem. 249:1974;4363-4382.
24. Park T.S., Gonzales E.R., Gidday J.M. Platelet-activating factor mediates ischemia-induced leukocyte-endothelial adherence in newborn pig brain. J. Cereb. Blood Flow Metab. 19:1999;417-424.
25. Sen C.K. Redox signaling and the emerging therapeutic potential of thiol antioxidants. Biochem. Pharmacol. 55:1998;1747-1758.
26. Smith K.J., Kapoor R., Felts P.A. Demyelination: the role of reactive oxygen and nitrogen species. Brain Pathol. 9:1999;69-92.
27. Stadler I. Oxygen consumption methods: xanthine oxidase and lipoxygenase. Methods Mol. Biol. 108:1998;3-14.
28. Sun Y., Oberley L.W., Oberley T.D., Elwell J.H., Sierra-Rivera E. Lowered antioxidant enzymes in spontaneously transformed embryonic mouse liver cells in culture. Carcinogen. 14:1993;1457-1463.
29. Symonyan M.A., Nalbandyan R.M. Interaction of hydrogen peroxide with superoxide dismutase from erythrocytes. FEBS Lett. 28:1972;22-24.
30. Weisiger A., Fridovich I. Superoxide dismutase. Organelle specificity. J. Biol. Chem. 248:1972;3582-3592.
31. Zhou J., Holtzman D.M., Weiner R.I., Mobley W.C. Expression of TrkA confers neuron-like responsiveness to nerve growth factor on an immortalized hypothalamic cell line. Proc. Natl. Acad. Sci. U.S.A. 91:1994;3824-3828.