-
2
-
-
0032401757
-
-
J. L. Bos, EMBO J. 17, 6776 (1998).
-
(1998)
EMBO J.
, vol.17
, pp. 6776
-
-
Bos, J.L.1
-
5
-
-
0024376173
-
-
_, Cancer Res. 49, 4682 (1989).
-
(1989)
Cancer Res.
, vol.49
, pp. 4682
-
-
-
7
-
-
0021170342
-
-
B. M. Willumsen, A. Christensen, N. L. Hubbert, A. G. Papageorge, D. R. Lowy, Nature 310, 583 (1984).
-
(1984)
Nature
, vol.310
, pp. 583
-
-
Willumsen, B.M.1
Christensen, A.2
Hubbert, N.L.3
Papageorge, A.G.4
Lowy, D.R.5
-
8
-
-
0030612937
-
-
M. W. Mayo et al., Science 278, 1812 (1997).
-
(1997)
Science
, vol.278
, pp. 1812
-
-
Mayo, M.W.1
-
11
-
-
0343900508
-
-
data not shown
-
C. Fenwick et al., data not shown.
-
-
-
Fenwick, C.1
-
14
-
-
0029985757
-
-
I. G. Macara, K. M. Lounsbury, S. A. Richards, C. McKiernan, D. Bar-Sagi, FASEB J. 10, 625 (1996).
-
(1996)
FASEB J.
, vol.10
, pp. 625
-
-
Macara, I.G.1
Lounsbury, K.M.2
Richards, S.A.3
McKiernan, C.4
Bar-Sagi, D.5
-
15
-
-
0025908897
-
-
A. Valencia, P. Chardin, A. Wittinghofer, C. Sander, Biochemistry 30, 4637 (1991).
-
(1991)
Biochemistry
, vol.30
, pp. 4637
-
-
Valencia, A.1
Chardin, P.2
Wittinghofer, A.3
Sander, C.4
-
16
-
-
0343900503
-
-
note
-
Construction of the phylogenic tree was done with the Phylip software package. The Ras similarity domains (corresponding to amino acids 5 to 164 of H-Ras) were aligned by ClustalW alignment and compared by parsimonious analysis with amino acid similarities and bootstrapping analysis. Although variations of the Ras family dendrogram were generated, κB-Ras1 and κB-Ras2 remained grouped with Ras/ Ral/Rap proteins.
-
-
-
-
18
-
-
0030869418
-
-
D. G. Winkler, J. C. Johnson, J. A. Cooper, A. B. Vojtek, J. Biol. Chem. 272, 24402 (1997).
-
(1997)
J. Biol. Chem.
, vol.272
, pp. 24402
-
-
Winkler, D.G.1
Johnson, J.C.2
Cooper, J.A.3
Vojtek, A.B.4
-
19
-
-
0342594779
-
-
note
-
Nucleotide binding to κB-Ras1 was performed by slot-blot analysis with bacterially expressed κB-Ras1 with a COOH-terminal His tag. κB-Ras1 was purified on nickel-agarose gel and dialyzed extensively in a buffer containing 1 mM EDTA before the nucleotide-binding assay (32).
-
-
-
-
21
-
-
1842301722
-
-
J. Cherfils et al., EMBO J. 16, 5582 (1997).
-
(1997)
EMBO J.
, vol.16
, pp. 5582
-
-
Cherfils, J.1
-
22
-
-
0343464715
-
-
unpublished observations
-
C. Fenwick and S. Ghosh, unpublished observations.
-
-
-
Fenwick, C.1
Ghosh, S.2
-
24
-
-
0343028890
-
-
note
-
6 for Jurkat cells) and grown for 24 hours before transfection with 1 μg of DNA with the Fugene6 method (Boehringer Mannheim). After -24 hours, selected culture cells were induced with TNF-α (10 ng/ml) or recombinant human IL-1β (10 ng/ml) or with phorbol 12-myristate 13-acetate (250 ng/ml) or ionomycin (10 ng/ml). All cell cultures were harvested 4 hours later, and luciferase activity was measured (Promega luciferase assay kit). Transfection control was measured with a renila luciferase vector with a thymidine kinase promoter or by protein concentration.
-
-
-
-
25
-
-
0030991201
-
-
H. Zhong, H. SuYang, H. Erdjument-Bromage, P. Tempst, S. Ghosh, Cell 89, 413 (1997).
-
(1997)
Cell
, vol.89
, pp. 413
-
-
Zhong, H.1
SuYang, H.2
Erdjument-Bromage, H.3
Tempst, P.4
Ghosh, S.5
-
26
-
-
0027960533
-
-
S. C. Sun, J. Elwood C. Beraud, W. C. Greene, Mol. Cell. Biol. 14, 7377 (1994).
-
(1994)
Mol. Cell. Biol.
, vol.14
, pp. 7377
-
-
Sun, S.C.1
Elwood, J.2
Beraud, C.3
Greene, W.C.4
-
27
-
-
0028986194
-
-
J. E. Thompson, R. J. Phillips, H. Erdjument-Bromage, P. Tempst, S. Ghosh, Cell 80, 573 (1995).
-
(1995)
Cell
, vol.80
, pp. 573
-
-
Thompson, J.E.1
Phillips, R.J.2
Erdjument-Bromage, H.3
Tempst, P.4
Ghosh, S.5
-
29
-
-
0030685825
-
-
F. Mercurio et al., Science 278, 860 (1997).
-
(1997)
Science
, vol.278
, pp. 860
-
-
Mercurio, F.1
-
30
-
-
0030613551
-
-
E. Zandi, D. M. Rothwarf, M. Delhase, M. Hayakawa, M. Karin, Cell 91, 243 (1997).
-
(1997)
Cell
, vol.91
, pp. 243
-
-
Zandi, E.1
Rothwarf, D.M.2
Delhase, M.3
Hayakawa, M.4
Karin, M.5
-
31
-
-
0343464714
-
-
note
-
Cells were harvested and blotted as previously described (25) with membranes probed with antibody to IκBα or IκBβ (C21 and C20; Santa Cruz Biotechnology, Santa Cruz, CA), polyclonal rabbit antibodies, or monoclonal mouse antibody to HA (12CA5). Immunoprecipitations were performed on transfected COS or 293 cells (3.5- and 10-cm plates, respectively), as previously described, with antibody to HA.
-
-
-
-
32
-
-
0031471341
-
-
A. S. Raw, D. E. Coleman, A. C. Gilman, S. R. Sprang, Biochemistry 36, 15660 (1997).
-
(1997)
Biochemistry
, vol.36
, pp. 15660
-
-
Raw, A.S.1
Coleman, D.E.2
Gilman, A.C.3
Sprang, S.R.4
-
33
-
-
0343028889
-
-
note
-
Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
-
-
-
-
34
-
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0343900500
-
-
note
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This work was supported by the Howard Hughes Medical Institute (HHMI) and NIH (S.G.) and by the National Creative Research Initiatives Program of the Korean Ministry of Science and Technology (J.W.L). C.F. was supported by Fonds Formation Chercheurs et Aide Recherche and HHMI, R.E.V. by Deutsche Forschungsgemeinschaft, and H.Z. by the Leukemia Society of America. We thank I. Douglas for technical help, P. J. Novick for the purified Sec4 protein, D. S. Na for helpful advice, and D. G. Schatz, D. Sengupta, H. J. Kim, and M. Solomon for careful reading of the manuscript.
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