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Initial attempts to couple 7 to the heptaamino-β-CD 4 core utilizing dicyclohexylcarbodiimide (DCC) and hydroxybenzotriazole or DCC and 1-hydroxy-7-azabenzotriazole as activating agents were unsuccessful, largely on account of difficulties encountered in removing the reaction byproducts. Although benzotriazol-1-yloxytripyrrolidinophosphonium hexafluorophosphate (Py-BOP) was found to be act as a suitable activator, the removal of the tripyrrolidinophosphonium oxide byproduct was far from straightforward.
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