Directed evolution of toluene dioxygenase from Pseudomonas putida for improved selectivity toward cis-indandiol during indene bioconversion

Metabolic Engineering

Volumn 2, Issue 4, 2000, Pages 339-348

  Zhang, Ningyan ^a   Stewart, Bruce G ^a   Moore, Jeffrey C ^a   Greasham, Randolph L ^a   Robinson, David K ^a   Buckland, Barry C ^a   Lee, Chanyong ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Directed evolution; Indene bioconversion; Pseudomonas; Toluene dioxygenase

Indexed keywords

1 INDENOL; BACTERIAL ENZYME; BACTERIUM MUTANT; BIOCONVERSION; CHEMICAL MUTAGENESIS; GENE PRODUCT; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INDENE DERIVATIVE; INDINAVIR; MOLECULAR EVOLUTION; POLYMERASE CHAIN REACTION; TOLUENE DIOXYGENASE; WILD RELATIVE;

ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS 1; PSEUDOMONAS; PSEUDOMONAS PUTIDA; TRIXIS;

EID: 0034503418     PISSN: 10967176     EISSN: None     Source Type: Journal    
DOI: 10.1006/mben.2000.0162     Document Type: Article

References (23)

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