Comparison of the kinetic properties of the lipid- and protein-kinase activities of the p110α and p110β catalytic subunits of class-Ia phosphoinositide 3-kinases

Biochemical Journal

Volumn 350, Issue 2, 2000, Pages 353-359

  Beeton, C A ^a   Chance, E M ^a   Foukas, L C ^a   Shepherd, P R ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

p85 ; PI 3 kinase

Indexed keywords

GROWTH FACTOR; ISOENZYME; LIPID KINASE; MEMBRANE PHOSPHOLIPID; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; PHOSPHOTRANSFERASE; PROTEIN KINASE; PROTEIN P110; PROTEIN SERINE KINASE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; AUTOPHOSPHORYLATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME REGULATION; ENZYME SUBSTRATE; ENZYME SUBUNIT; HUMAN; HUMAN CELL; MICHAELIS CONSTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REACTION ANALYSIS; REACTION TIME;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ANDROSTADIENES; ANIMALS; CATALYSIS; CATALYTIC DOMAIN; CATTLE; CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME INHIBITORS; HUMANS; KINETICS; PEPTIDES; PHOSPHORYLATION; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PRECIPITIN TESTS; PROTEIN ISOFORMS; PROTEIN KINASES; RECOMBINANT PROTEINS; TIME FACTORS; TRANSFECTION;

EID: 0034283188     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3500353     Document Type: Article

References (41)

1. The role of phosphoinositide 3-kinase lipid products in cell function
2. Signalling through phosphoinositide 3-kinases: The lipids take centre stage
3. Phosphoinositide 3-kinase: The key switch mechanism in insulin signalling
4. Signalling through the lipid products of phosphoinositide 3-OH kinase
5. The role of PI 3-kinase in insulin action
6. Using structure to define the function of phosphoinositide 3-kinase
7. Structure and function of phosphoinositide 3-kinase
8. The G βγsensitivity of a PI3K is dependent upon a tightly associated adaptor, p101
9. Phosphatidylinositol 3-kinase: Structure and expression of the 110 kD catalytic subunit
10. Cloning of a novel, ubiquitously expressed human phosphatidyl inositol 3-kinase and identification of binding site to p85
11. p110γ, a novel phosphoinositide 3-kinase in leukocytes
12. Actin filaments participate in the relocalization of phosphatidylinositol 3-kinase to glucose transporter containing compartments and in the stimulation of glucose uptake in 3T3-L1 adipocytes
13. Phosphatidylinositol 3-OH kinases are Rab5 effectors
14. Differential mRNA expression and subcellular locations of PI 3-kinase isoforms in sympathetic and sensory neurons
15. Proliferative defects and embryonic lethality in mice homozygous for a deletion in the p110α subunit of phosphoinositide 3-kinase
16. Heterodimeric phosphoinositide 3-kinase consisting of p85 and p110β is synergistically activated by the βγ subunits of G-proteins and phosphotyrosyl peptide
17. Autophosphorylation of p110δ phosphoinositide 3-kinase: A new paradigm for the regulation of lipid kinases in vitro and in vivo
18. Differential effects of constitutively active phosphatidylinositol 3-kinase on glucose transport, glycogen synthase activity and DNA synthesis in 3T3-L1 adipocytes
19. Distinct roles for the p110α and hVPS34 phosphatidylinositol 3'-kinases in vesicular trafficking, regulation of actin cytoskeleton and mitogenesis
20. A function for phosphatidylinositol 3-kinase beta (p85α-p110β) in fibroblasts during mitogenesis: Requirement for insulin and lysophosphatidic acid mediated signal transduction
21. Distinct PI(3)Ks mediate mitogenic signalling and cell migration in macrophages
22. Overexpression of the catalytic p110α of phosphatidylinositol 3-kinase increases glucose transport activity and translocation of glucose transporters in 3T3-L1 adipocytes
23. p110β is up-regulated during differentiation of 3T3-L1 cells and contributes to the highly insulin-responsive glucose transport activity
24. A tightly associated serine/threonine protein kinase regulates phosphoinositide 3-kinase activity
25. PI 3-kinase is a dual specificity enzyme: Autoregulation by an intrinsic protein-serine kinase activity
26. The SH3 and BH domains of the p85α adapter subunit play a critical role in regulating class la phosphoinositide 3-kinase function
27. Binding of a diphosphotyrosine containing peptide that mimics activated platelet derived growth factor receptor b induces oligomerization of phosphatidylinositol 3-kinase
28. Compartment specific regulation of phosphoinositide 3-kinase by PDGF and insulin in 3T3-L1 adipocytes
29. Insulin activates the α-isoform of class II phosphoinositide 3-kinase
30. Kinetic analysis of the coding properties of the 0-6 methylguanine in DNA - The crucial role of the conformation of the phosphodiester bond
31. Regulation of the p85/p110 phosphatidylinositol 3'-kinase: Stabilization and inhibition of the p110α catalytic subunit by the p85 regulatory subunit
32. Biochemical characterization of the free catalytic p110α and the complexed heterodimeric p110α/p85α forms of the mammalian phosphatidylinositol 3-kinase
33. Purification and characterization of phosphoinositide 3-kinase from rat liver
34. p85/p110-type phosphatidylinositol kinase phosphorylates not only the D3 but also the D4 position of the inositol ring
35. Phosphatidylinositol 4-phosphate synthesis in immunoisolated caveolae like vesicles and low buoyant density non caveolar membranes
36. Phosphoinositides and phosphoinositide utilizing enzymes in detergent insoluble lipid domains
37. Compartmentalization of phosphatidylinositol 4,5 bisphosphate in low density membrane domains in the absence of caveolin
38. The caveolae membrane system
39. Different effects of insulin and platelet derived growth factor on phosphatidylinositol 3-kinase at the subcellular level in 3T3-L1 adipocytes
40. The phosphatidylinositol 3-kinase serine kinase phosphorylates IRS-1: Stimulation by insulin and inhibition by wortmannin
41. Insulin receptor substrate-1 is phosphorylated by the serine kinase activity of phosphatidylinositol 3-kinase