An update of pTRIDENT multicistronic expression vectors: pTRIDENTs containing novel streptogramin-responsive promoters

Biotechnology Progress

Volumn 16, Issue 5, 2000, Pages 724-735

  Moser, S ^a   Schlatter, S ^a   Fux, C ^a   Rimann, M ^a   Bailey, J E ^a   Fussenegger, M ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CYAN FLUORESCENT PROTEIN; PTRIDENT MULTICISTRONIC EXPRESSION VECTOR; RED FLUORESCENT PROTEIN; STREPTOGRAMIN RESPONSIVE PROMOTER; STREPTOMYCES PRISTINAESPIRALIS; YELLOW FLUORESCENT PROTEIN; ZEOCIN RESISTANCE;

ALKALINE PHOSPHATASE; CYAN FLUORESCENT PROTEIN; EXPRESSION VECTOR; MAMMAL CELL; PROMOTER REGION; PROUROKINASE; PTRIDENT VECTOR; RED FLUORESCENT PROTEIN; REPORTER GENE; STREPTOGRAMIN; YELLOW FLUORESCENT PROTEIN; ZEOCIN;

BACTERIA; BIOMARKERS; CELLS; FLUORESCENCE; GENES; METABOLISM; PROTEINS;

GENETIC ENGINEERING;

GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; PROMOTER REGIONS (GENETICS); VIRGINIAMYCIN;

MAMMALIA; STREPTOMYCES PRISTINAESPIRALIS;

EID: 0034281609     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp000077r     Document Type: Article

References (60)

1. A versatile synthetic dimerizer for the regulation of protein-protein interactions
2. RNA-protein interactions in regulation of picornavirus RNA translation
3. The secreted alkaline phosphatase: A powerful new quantitative indicator of gene expression in eukaryotic cells
4. PDGF2/C SIS mRNA leader contains a differentiation linked internal ribosomal entry site (D IRES)
5. Modulation of erythropoietin delivery from engineered muscles in mice
6. Comparison of picornaviral IRES-driven internal initiation in cultured cells of different origins
7. A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus
8. Definition of the simian virus 40 early promoter region and demonstration of a host range bias in the enhancement effect of the simian virus 40 72 bp repeat
9. Use of green fluorescent protein variants to monitor gene transfer and expression in mammalian cells
10. The ubiquitin-mediated proteolytic pathway and mechanisms of energy-dependent intracellular protein degradation
11. Controlling mammalian gene expression with small molecules
12. Inhibition of protein synthesis by streptogramins and related antibiotics
13. Green fluorescent protein reporter microplate assay for high-throughput screening of compounds against Mycobacterium tuberculosis
14. Construction and characterization of polycistronic retrovirus vectors for sustained and high-level coexpression of apolipoprotein A-I and lecithin-cholesterol acetyltransferase
15. Use of tetracycline-controlled gene expression systems to study mammalian cell cycle
16. Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter
17. The variability in activity of the universally expressed human cytomegalovirus immediate early gene 1 enhancer/promoter in transgenic mice
18. Autoregulated multicistronic expression vectors provide one-step cloning of regulated product gene expression in mammalian cells
19. A novel cytostatic process enhances the productivity of Chinese hamster ovary cells
20. pTRIDENT, a novel vector family for tricistronic gene expression in mammalian cells
21. pQuattro vectors allow one-step transfection and auto-selection of quattrocistronic artificial mammalian operons
22. Regulated multicistronic expression technology for mammalian metabolic engineering
23. Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells
24. Regulated multicistronic expression technology for mammalian metabolic engineering
25. Genetic optimization of recombinant glycoprotein production by mammalian cells
26. Regulated overexpression of the survival factor bcl-2 in CHO cells increases viable cell density in batch culture and decreases DNA release in extended fixed-bed cultivation
27. Modifications of vectors pEF-BOS, pcDNA1 and pcDNA3 result in improved convenience and expression
28. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters
29. The ubiquitin system
30. GFP-expressing mammalian cells for fast, sensitive, noninvasive cell growth assessment in a kinetic mode
31. Genetic regulatory mechanisms in the synthesis of proteins
32. Improved vectors for the stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMCV virus
33. Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice
34. Low-M(r) urokinase-type plasminogen activator as a reporter protein
35. Fluorescent proteins from nonbioluminescent Anthozoa species
36. Construction and characterization of single-transcript tricistronic retroviral vectors using two internal ribosome entry sites
37. 2+ based on green fluorescent proteins and calmodulin
38. pEF-BOS, a powerful mammalian expression vector
39. Liver abscesses in feedlot cattle: A review
40. Negative and positive regulation by a short segment in the 5'-flanking region of the human cytomegalovirus major immediate-early gene
41. Ecdysone-inducible gene expression in mammalian cells and transgenic mice
42. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells
43. Crystal structure of the Aequorea victoria green fluorescent protein
44. Primary structure of the Aequorea victoria green fluorescent protein
45. Engineering of coordinated up- and down-regulation of two glycosyltransferases of the O-glycosylation pathway in Chinese hamster ovary (CHO) cells
46. On-line green fluorescent protein sensor with LED excitation
47. Bicistronic vector for the creation of stable mammalian cell lines that predisposes all antibiotic-resistant cells to express recombinant protein
48. A humanized system for pharmacologic control of gene expression
49. Stress-activated expression of a Streptomyces pristinaespiralis multidrug resistance gene (ptr) in various Streptomyces spp. and Escherichia coli
50. Unusual regulatory mechanism for a Streptomyces multidrug resistance gene, ptr, involving three homologous protein-binding sites overlapping the promoter region
51. The cytomegalovirus enhancer: A pan-active control element in transgenic mice
52. mRNA cap binding proteins: Essential factors for initiating translation
53. The human ubiquitin C promoter directs high ubiquitous expression of transgenes in mice
54. Functional dissection of VP16, the transactivator of Herpes simplex virus immediate early gene expression
55. The green fluorescent protein
56. Isolation and characterization of the human chromosomal gene for the polypeptide chain-elongation factor-1α
57. Engineered glycoforms of an antineuroblastoma IgG1 with optimized antibody-dependent cellular cytotoxic activity
58. Tetracycline-regulated overexpression of glycosyltransferases in Chinese hamster ovary cells
59. CobA, a red fluorescent transcriptional reporter for Escherichia coli, yeast, and mammalian cells
60. Three-color flow cytometry analysis of tricistronic expression of eBFP, eGFP, and eYFP using EMCV-IRES linkages