Engineering of coordinated up- and down-regulation of two glycosyltransferases of the O-glycosylation pathway in Chinese hamster ovary (CHO) cells

Biotechnology and Bioengineering

Volumn 68, Issue 3, 2000, Pages 239-244

  Prati, Elisabetta G P ^a   Matasci, Mattia ^c   Suter, Tobias B ^c   Dinter, Andre ^c   Sburlati, Adriana R ^c   Bailey, James E ^c  

^a ETH ZURICH   (Switzerland)

^b UNIVERSITY OF ZURICH   (Switzerland)

^c NONE

Author keywords

C2GnT over expression; CHO cells; ST3Gal I inhibition

Indexed keywords

BIOSYNTHESIS; CATALYST ACTIVITY; CELL IMMOBILIZATION; ENZYME INHIBITION; ENZYME KINETICS; GENES; GLYCOLS; METABOLISM;

GLYCOSYLATION; GLYCOSYLTRANSFERASES; HAMSTER OVARY CELLS;

CLONING;

GLYCOSYLTRANSFERASE; TETRACYCLINE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BIOENGINEERING; CHO CELL; DOWN REGULATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME REGULATION; GENE OVEREXPRESSION; GLYCOSYLATION; MOLECULAR CLONING; NONHUMAN;

ALKALINE PHOSPHATASE; AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, CD15; ANTISENSE ELEMENTS (GENETICS); BASE SEQUENCE; CHO CELLS; CRICETINAE; DOWN-REGULATION; GLYCOSYLATION; HUMANS; MOLECULAR SEQUENCE DATA; N-ACETYLGLUCOSAMINYLTRANSFERASES; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SIALYLTRANSFERASES; TETRACYCLINE; UP-REGULATION;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; MAMMALIA; RODENTIA;

EID: 0034607630     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000505)68:3<239::AID-BIT1>3.0.CO;2-7     Document Type: Article

References (18)

1. Bailey JE, Prati EGP, Jean-Mairet J, Sburlati AR, Umaña P. 1998. Engineering glycosylation in animal cells. In: Merten O-M, Perrin P, Griffiths B, editors. New developments and new applications in animal cell technology. Boston: Kluwer Academic. pp 5-23.
2. Berger J, Hauber J, Hauber R, Geiger R, Cullen BR. 1988. Secreted placental alkaline phosphatase: A powerful new quantitative indicator of gene expression in eukaryotic cells. Gene 66:1-10.
3. Bierhuizen MFA, Fukuda M. 1992. Expression of a cDNA encoding UDP-GlcNAc:Ga1β1-3-GalNAc-R (GlcNAc to GalNAc) β1-6GlcNAc transferase by gene transfer into CHO cells expressing polyoma large tumor antigen. Proc Natl Acad Sci USA 89:9326-9330.
4. Chang ML, Eddy RL, Shows TB, Lau JTY. 1995. Three genes that encode human β-galactosidase α2,3sialyltransferases. Structural analysis and chromosomal mapping studies. Glycobiol 3:319-325.
5. Ferrari J, Gunson J, Lofgren J, Krummen L, Warner TG. 1998. Chinese hamster ovary cells with constitutively expressed sialidase antisense RNA produce recombinant DNase in batch culture with increased sialic acid. Biotechnol Bioeng 60:589-595.
6. Fussenegger M, Mazur X, Bailey JE. 1997. pTRIDENT, a novel vector family for tricistronic gene expression in mammalian cells. Biotechnol Bioeng 57:1-10.
7. Gillespie W, Kelm S, Paulson JC. 1992. Cloning and expression of the Gal-β-1,3-GalNAc α-2,3-sialyltransferase. J Biol Chem 267: 21004-21010.
8. Gossen M, Bujard H. 1992. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA 89:5547-5551.
9. Kitagawa H, Paulson JC. 1994. Differential expression of five sialyltransferase genes in human tissues. J Biol Chem 269:17872-17878.
10. Kurosawa N, Hamamoto T, Inoue M, Tsuji S. 1995. Molecular cloning and expression of chick Gal-β-1,3-GalNAc α-2,3-sialyltransferase. Biochim Biophys Acta 1244:216-222.
11. Lasky LA, Singer MS, Dowbenko D, Imai Y, Henzel WJ, Grimley C, Fennie C, Gillett N, Watson SR, Rosen SD. 1992. An endothelial ligand for L-selectin is a novel mucin-like molecule. Cell 69:927-938.
12. Lasky LA. 1995. Selectin-carbohydrate interactions and the initiation of the inflammatory response. Ann Rev Biochem 64:113-139.
13. Lee Y-C, Kurosawa N, Hamamoto T, Nakaoka T, Tsuji S. 1993. Molecular cloning and expression of Galβ1,3GalNAcα2,3-sialyltransferase from mouse brain. Eur J Biochem 216:377-385.
14. Monteith DK, Levin AA. 1999. Synthetic oligonucleotides: The development of antisense therapeutics. Toxicol-Pathol 27:8-13.
15. Parekh RH, Edge CJ. 1994. Selectins-glycoproteins targets for therapeutic intervention in inflammation. TIBTECH 12:339-345.
16. Prati EGP, Scheidegger P, Sburlati AR, Bailey JE. 1998. Antisense strategies for glycosylation engineering of Chinese hamster ovary (CHO) cells. Biotechnol Bioeng 59:445-450.
17. Roush W. 1997. Antisense aims for a renaissance. Science 276:1192-1193.
18. Whitehouse C, Burchell J, Gschmeissner S, Brockhausen I, Lioyd KO, Taylor-Papdimitriou J. 1997. A transfected sialyltransferase that is elevated in breast cancer and localizes to the medial/trans-Golgi apparatus inhibits the development of core-2-based O glycan. J Cell Biol 137:1229-1241.