Procedures to Characterize and Study P2Z/P2X7 Purinoceptor: Flow Cytometry as a Promising Practical, Reliable Tool

Memorias do Instituto Oswaldo Cruz

Volumn 95, Issue 3, 2000, Pages 415-428

  Nihei, Oscar Kenji ^a   Savino, Wilson ^a   Alves, Luiz Anastacio ^a  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

Author keywords

Ethidium bromide; Extracellular ATP; Flow cytometry; P2X7 receptor; Purinergic receptors

Indexed keywords

PURINE P2 RECEPTOR; PURINE P2X7 RECEPTOR;

ANIMAL; CHEMISTRY; CLASSIFICATION; DENDRITIC CELL; FLOW CYTOMETRY; HUMAN; METHODOLOGY; MOUSE; RAT; REVIEW;

ANIMALS; DENDRITIC CELLS; FLOW CYTOMETRY; HUMANS; MICE; RATS; RECEPTORS, PURINERGIC P2;

EID: 0034183381     PISSN: 00740276     EISSN: None     Source Type: Journal    
DOI: 10.1590/S0074-02762000000300019     Document Type: Article

References (120)

1. Abbracchio MP, Burnstock G 1994. Purinoceptors: Are there families of P2X and P2Y purinoceptors? Pharmacol Ther 64: 445-475.
2. Albuquerque C, Oliveira SMC, Coutinho-Silva R, Oliveira-Castro GM, Persecchini PM 1993. ATP and UTP-induced currents in macrophages and macrophages polykaryons. Am J Physiol 265: C1663-C1673.
3. Alonso-Torrez SR, Trautmann A 1993. Calcium response elicited by nucleotides in macrophages. J Biol Chem 268: 18640-18647.
4. Alves LA, Coutinho-Silva R, Savino W 1999. Extracellular ATP: a further modulator in neuroendocrine control of the thymus. Neuroimmunomodulation 6: 81-9.
5. 2) in ductal cells of rat submandibular gland. J Biol Chem 273: 30208-30217.
6. Ballerini P, Rathbone MP, Di Iorio P, Renzetti A, Giuliani P, D'alimonte I, Trubiani O, Caciagli F, Ciccarelli R 1996. Rat astroglial P2z(P2X7) receptors regulate intracellular calcium and purine release. Neuroreport 7: 2533-7.
7. Blanchard DK, Hoffman LS, Djeu JY 1995. Inhibition of extracellular ATP-mediated lysis of human macrophages by calmodulin antagonists. J Cell Biochem 57: 452-464.
8. Brake AJ, Wagenbach MJ, Julius D 1994. New structural motif for ligand-gated ion channels defined by an ionotropic ATP receptor. Nature 371: 519-523.
9. Brandle U, Spielmanns P, Osteroth R, Sim J, Surprenant A, Buell G, Ruppersberg JP, Plinkert PK, Zenner HP, Glowatzki E 1997. Desensitization of the P2X(2) receptor control led by alternative splicing. FEBS Lett 404: 294-298.
10. Bo X, Zhang Y, Nassar M, Burnstock G, Schoepfer R 1995. A P2X purinoceptor cDNA conferring a novel pharmacological profile. FEBS Lett 375: 129-133.
11. Buell G, Chessell IP, Michel AD, Collo G, Salazzo M, Herren S, Gretener D, Grahames C, Kaur R, Kosco-Vilbois MH, Humphrey PPA 1998. Blockade of human P2X7 receptor function with a monoclonal antibody. Blood 92: 3521-3528.
12. Buell G, Lewis C, Collo G, North RA, Surprenant A 1996. An antagonist-insensitive P2X receptor expressed in epithelia and brain. EMBO J 15: 55-62.
13. Burnstock G 1971. Neural nomenclature. Nature 229: 282-283.
14. Burnstock G 1978. A bases for distinguishing of two types of purinergic receptors. In Cell Membrane Receptors for Drugs and Hormones, a Multidisciplinarv Approach, RW Straub and L Bolis, New York, p: 107-118.
15. Burnstock G 1996. Introduction: Purinergic transmission. Semin Neurosci 8: 171-174.
16. Burnstock G 1997. The past, present and future of purine nucleotides as signaling molecules. Neuropharmacology36: 1127-1139.
17. Cario-Toumaniantz C, Loirand G, Ladoux A, Pacaud P 1998. P2X7 receptor activation-induced contraction and lysis in human saphenous vein smooth muscle. Circ Res 83: 196-203.
18. 2- type purinoceptors of vertebrates. J Comp Physiol 158: 331-338.
19. Chen CC, Akopian AN, Sivilotti L, Colquhoun D, Burnstock G, Wood JN 1995. A P2X purinoceptor expressed by a subset of sensory neurons. Nature 377: 428-431.
20. Chessell IP, Michel AD, Humphrey P 1997. Properties of the pore-forming P2X7 purinoceptor in mouse NTW8 microglial cells. Br J Pharmacol 121: 1429-1437.
21. Chessell IP, Michel AD, Humphrey PPA 1998b. Effects of antagonists at the human recombinant P2X7 receptor. Br J Pharmacol 124: 1314-1320.
22. Chessell IP, Simon J, Hibell AD, Barnard EA, Humphrey PPA 1998a. Cloning and functional characterization of the mouse P2X7 receptor. FEBS Letters 439: 26-30.
23. Chiozzi P, Murgia M, Falzoni S, Ferrari D, Di Virgilio F 1996. Role of the purinergic P2Z receptor in spontaneous cell death in J774 macrophage cultures. Biochem Biophys Res Commun 218: 176-181.
24. Chiozzi P, Sanz JM, Ferrari D, Falzoni S, Aleotti, A, Buell GN, Collo G, Di Virgilio F 1997. Spontaneous cell fusion in macrophage cultures expressing high levels of the P2Z/P2X7 receptor. J Cell Biol 138: 697-706.
25. Christoffersen BC, Hug MJ, Novak I 1998. Different purinergic receptors lead to intracellular calcium increases in pancreatic ducts. Pflugers Arch 436: 33-9.
26. Chused TM, Apasov S, Sitkovsky M 1996. Murine T lymphocytes modulate activity of an ATP-activated P2z- type purinoceptor during differentiation. J Immunol 157: 1371-1380.
27. Collo G, Neidhart S, Kawashima E, Kosco-Vilbois M, North RA, Buell G 1997. Tissue distribution of the P2X7 receptor. Neuropharmacology 36: 1277-1283.
28. Collo G, North RA, Kawashima E, Merlo-Pich E, Neidhart S, Surprenant A, Buell G 1996. Cloning OF P2X5 and P2X6 receptors and the distribution and properties of an extended family of ATP-gated ion channels. J Neurosci 16: 2495-2507.
29. Coutinho-Silva R, Persechini PM 1997. P2Z purinoceptor-associated pores induced by extracellular ATP in macrophages and J774 cells. Am J Physiol 273: C1793-C1800.
30. Coutinho-Silva R, Alves LA, Campos-De-Carvalho AC, Savino W, Persechini PM 1996a. Characterization of P2Z purinergic receptors on phagocytic cells of the thymic reticulum in culture. Biochim Biophys Acta 1280: 217-222.
31. Coutinho-Silva R, Alves LA, Savino W, Persechini PM 1996b. A cation non-selective channel induced by extracellular ATP in macrophages and phagocytic cells of the thymic reticulum. Biochim Biophys Acta 1278: 125-130.
32. Coutinho-Silva R, Persechini PM, Bisaggio RD, Perfettini JL, Neto AC, Kanellopoulos JM, Motta-Ly I, Dautry-Varsat A, Ojcius DM 1999. P2Z/P2X7 receptor-dependent apoptosis of dendritic cells. Am J Physiol 276: C1139-47
33. Devreotes PN, Zigmond SH 1988. Chemotaxis in eukaryotic cells: a focus on leukocytes and Dictyostelium. Ann Rev Cell Biol 4: 649-686.
34. 4 receptor gene is alternatively spliced. Gene 207: 259-266.
35. Di Virgilio 1995. The P2Z purinoceptor: an intriguing role in immunity, inflammation and cell death. Immunol Today 16: 524-528.
36. Drury AN, Szent-Györgyi A 1929. The physiological activity of adenine compounds with especial reference to their action upon the mammalian heart. J Physiol 68: 213-237.
37. Dubyak GR, El-Moatassim C 1993. Signal transduction via P2-purinergic receptors for extracellular ATP and other nucleotides. Am J Physiol 265: C577-C606.
38. El-Moatassim C, Dubyak GR 1992. A novel pathway for the activation of phospholipase D by P2z purinergic receptors in BAC1.2F5 macrophages. J Biol Chem 267: 23664-73.
39. El-Moatassim C, Dubyak GR 1993. Dissociation of the pore-forming and phospholipase D activities stimulated via P2z purinergic receptors in BAC1.2F5 macrophages. J Biol Chem 268: 15571-15578.
40. El-Moatassim C, Bernad N, Mani J, Dornand J 1989. Extracellular ATP induces a nonspecific permeability of thymocyte plasma membranes. Biochem Cell Biol 67: 495-502.
41. -4 permeabilizes thymocytes not only to cations but also to low-molecular-weight solutes. Eur J Pharmacol 181: 111-118.
42. Ferrari D, Villalba M, Chiozzi P, Falzoni S, Ricciardi-Castagnoli P, Di Virgilio F 1996. Mouse microglial cells express a plasma membrane pore gated by extracellular ATP. J Immunol 156: 1531-1539.
43. Ferrari D, Wesselborg S, Bauer MKA, Schulze-Osthoff K 1997. Extracellular ATP activates transcription factor NF-kappaB through the P2Z purinoceptor by selectively targeting NF-KappaB p65. J Cell Biol 139: 1635-1643.
44. Fredholm BB, Abbracchio MP, Burnstock G, Daly JW, Harden K, Jacobson KA, Leff P, Willians M 1994. Nomenclature and classification of purinoceptors. Pharmacol Rev 46: 143-156.
45. Foresta C, Rossato M, Nogara A, Gottardello F, Bordon P, Di Virgilio 1996. Role of P2-purinergic receptors in rat Leydig cell steroidogenesis. Biochem J 320: 499-504.
46. 2+ induced by purinoceptors in parotid acinar cells of the rat. Eur J Pharmacol 336: 89-97.
47. Gan BS, Krump E, Shrode LD, Grinstein S 1998. Loading pyranine via purinergic receptors or hypotonic stress for measurement of cytosolic pH by imaging. Am J Physiol 275: C1158-C1166.
48. Garcia-Guzman M, Soto F, Gomez-Hernandez JM, Lund PE, Stuhmer W 1997b. Characterization of recombinant human P2X4 receptor reveals pharmacological differences to the rat homologue. Mol Pharmacol 51: 109-118.
49. Garcia-Guzman M, Soto F, Laube B, Stuhmer W 1996. Molecular cloning and functional expression of a novel rat heart P2X purinoceptor. FEBS Lett 388: 123-127.
50. Garcia-Guzman M, Stuhmer W, Soto F 1997a. Molecular characterization and pharmacological properties of the human P2X3 purinoceptor. Brain Res Mol Brain Res 47: 59-66.
51. Gargett CE, Wiley JS 1997. The isoquinoline derivative KN-62 a potent antagonist of the P2Z-receptor of human lymphocytes. Br J Pharmacol 120: 1483-1490.
52. Gargett CE, Cornish EJ, Wiley JS 1996. Phospholipase D activation by P2Z-purinoceptor agonists in human lymphocytes is dependent on bivalent cation influx. Biochem J 313: 529-535.
53. Gonzalez FA, Ahmed AH, Lustig KD, Erb L, Weisman GA 1989. Permeabilization of transformed mouse fibroblast by 3′-O-(4-benzoyl)benzoyl adenosine 5′-triphosphate and the desensitization of the process. J Cell Physiol 139: 109-115.
54. Gordon JL 1986. Extracellular ATP: effects, sources and fate. Biochem J 233: 309-319.
55. Grafe P, Mayer C, Takigawa T, Kamleiter M, Sanchez-Brandelik 1999. Confocal calcium imaging reveals an ionotropic P2 nucleotide receptor in the paranodal membrane of rat Schwann cells. J Physiol 515: 377-383.
56. Greenberg S, Di Virgilio F, Steinberg TH, Silverstein SC 1988. Extracellular nucleotides mediate Ca2+ fluxes in J774 macrophages by two distinct mechanisms. J Biol Chem 263: 10337-10343.
57. Hansen MA, Barden JA, Vladimir JB, Keay KA, Bennett MR 1997. Structure motif and characteristics of the extracellular domain of P2X receptors. Biochem Biophys Res Commun 236: 670-675.
58. 2-Purinergic Receptors: Subtype-associated signaling responses and structure. Annu Rev Pharmacol Toxicol 35: 541-79.
59. Hickman SE, Khoury JEL, Greenberg S, Schieren I, Silverstein SC 1994. P2z adenosine triphosphate receptor activity in cultured human monocyte-derived macrophages. Blood 84: 2452-2456.
60. 7 Nucleotide Receptor and associated phospholipase D activity by lipopolysaccharide and IFN-gamma in the human THP-1 monocytic cell line. J Immunol 157: 5627-5637.
61. Humphreys BD, Dubyak GR 1998. Modulation of P2X7 nucleotide receptor expression by pro- and antiinflamatory stimuli in THP-1 monocytes. J Leukoc Biol 64: 265-273.
62. Jaffar ZH, Pearce FL 1993. Some characteristics of the ATP-induced histamine release from and permeabilization of rat mast cells. Agents actions 40: 18-27.
63. Jamieson GP, Snook MB, Thurlow PJ, Wiley JS 1996. Extracellular ATP causes loss of L-selectin from human lymphocytes via occupancy of P2z purinoceptors. J Cell Physiol 166: 637-642.
64. Kaiho H, Kimura J, Matsuoka I, Kumasaka T, Nakanishi H 1996. ATP-activated nonselective cation current in NG108-15 cells. J Neurochem 67: 398-406.
65. Kaiho H, Matsuoka I, Kimura J, Nakanishi H 1998. Identification of P2X7(P2z) receptor in N18TG-2 cells and NG108-15 cells. J Neurochem 70: 951-7.
66. King BF, Townsend-Nicholson A, Burnstock G 1998. Metabotropic receptors for ATP and UTP: exploring the correspondence between native and recombinant nucleotide receptors. Trends Pharmacol Sci 19: 506-514.
67. Kohler G, Milstein C 1975. Continuous cultures of fused cells secreting antibodies of predefined specificity. Nature 256: 495-497.
68. Lê K, Paquet M, Nouel D, Babinski K, Séguela P 1997. Primary structure and expression of a naturally truncated human P2X ATP receptor subunit from brain and immune system. FEBS Letters 418: 195-199.
69. Lewis C, Neidhart S, Holy C, North RA, Buell G, Surprenant A 1995. Coexpression of P2X2 and P2X3 receptor subunits can account for ATP-gated currents in sensory neurons. Nature 377: 432-435.
70. Lin J, Krishnaraj R, Kemp RG 1985. Exogenous ATP enhances calcium influx in intact thymocytes. J Immunol 135: 3403-3410.
71. Longhurst PA, Schwegel T, Folander K, Swanson R 1996. The human P2x1 receptor: molecular cloning, tissue distribution, and localization to chromosome 17. Biochim Biophys Acta 1308: 185-188.
72. Markwardt F, Lohn M, Bohm T, Klapperstuck M 1997. Purinoceptor-operated cationic channels in human B lymphocytes. J Physiol 498: 143-151.
73. Mcmillian MK, Soltoff SP, Cantley LC, Rudel RA, Talamo Br 1993. Two distinct cytosolic calcium response to extracellular ATP in rat parotide acinar cells. Br J Pharmacol 108: 453-461.
74. Melamed MR, Mullaney PF, Shapiro HM 1991. An historical review of the development of flow cytometers and sorters. In MR Melamed, T Lindmo and ML Mendelsohn (eds), Flow Cytometry and Sorting, 2nd ed., Wiley-Liss, Inc, New York, p. 1-9.
75. Michel AD, Chesseli IP, Hibell AD, Simon J, Humphrey PPA 1998. Identification and characterization of an endogenous P2X7(P2z) receptor in CHO-K1 cells. Br J Pharmacol 125: 1194-1202.
76. Munerati M, Cortesi R, Ferrari D, Di Virgilio, Nastruzzi C 1994. Macrophages loaded with doxorubicin by ATP-mediated permeabilization: potential carriers for antitumor therapy. Biochim Biophys Acta 1224: 269-276.
77. Murgia M, Hanau S, Pizzo P, Rippa M, Di Virgilio F 1993. Oxidized ATP: An irreversible inhibitor of macrophage purinergic P2Z receptor. J Biol Chem 268: 8199-8203.
78. Mutini C, Falzoni S, Ferrari D, Chiozzi P, Morelli A, Baricordi OR, Collo G, Ricciardi-Castagnoli P, Di Virgilio F 1999. Mouse dendritic cells express the P2X7 purinergic receptor: characterization and possible participation in antigen presentation. J Immunol 163: 1958-65.
79. Nagy P, Panyi G, Jenei A, Bene L, Gáspár R, Matkó J, Damjanovich S 1995. Ion channel activities regulate transmembrane signaling in thymocyte apoptosis and T-cell activation. Immunol Lett 44: 91-95.
80. Naumov AP, Kaznacheyeva EV, Kiselyov KI, Kuryshev YA, Mamin AG, Mozhayeva GN 1995. ATP-activated inward current and calcium-permeable channels in rat macrophage plasma membranes. J Physiol 486: 323-337.
81. 2+-permeable channels in rat peritoneal macrophages. FEBS Letters 313: 285-287.
82. Neher E, Sakmann B 1976. Single channel currents recorded from membrane of denervated frog muscle fiber. Nature 260: 779-802.
83. Nuttle LC, Dubyak GR 1994. Differential activation of cation channels and non-selective pores by macrophage P2z purinergic receptors expressed in Xenopus oocytcs. J Biol Chem 269: 13988-13996.
84. Olah ME, Stiles GL 1995. Adenosine receptor subtypes: Characterization and therapeutic regulation. Annu Rev Pharmacol Toxicol 35: 581-606.
85. Parker JC, Snow RL 1972. Influence of external ATP on permeability and metabolism of dog red blood cells. Am J Physiol 56: 888-893.
86. Persechini PM, Bisaggio RC, Alves-Neto JL, Coutinho-Silva R 1998. Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilization. Braz J Med Biol Res 31: 25-34.
87. Petrou S, Ugur M, Drummond RM, Singer JJ, Walsh JV 1997. P2X7 purinoceptor expression in Xenopus oocytes is not sufficient to produce a pore-forming P2Z-like phenotype. FEBS Lett 411: 339-345.
88. 2+ increases plasma membrane permeability in murine macrophages. J Biol Chem 265: 5635-5639.
89. Pizzo P, Murgia M, Zambon A, Zanovello P, Bronte V, Pietrobon D, Di Virgilio F 1992. Role of P2z purinergic receptors in ATP-mediated killing of tumor necrosis factor (TNF)-sensitive and TNF-resistant L929 fibroblasts. J Immunol 149: 3372-3378.
90. Pizzo P, Zanovello P, Bronte V, Di Virgilio F 1991. Extracellular ATP causes lysis of mouse thymocytes and activates a plasma membrane ion channel. Biochem J 274: 139-144.
91. Ralevic V, Burnstock G 1998. Receptors for purines and pyrimidines. Pharmacol Rev 50: 413-491.
92. Rassendren F, Buell GN, Virginio C, Collo G, North RA, Surprenant A 1997. The permeabilizing ATP receptor, P2X7. Cloning and expression of a human cDNA. J Biol Chem 272: 5482-5486.
93. Ross PE, Ehring GR, Cahalan MD 1997. Dynamics of ATP-induced calcium signaling in single mouse thymocytes. J Cell Biol 138: 987-998.
94. Salih SG, Housley GD, Burton LD, Greenwood D 1998. P2XT receptor subunit expression in a subpopulation of cochlear type I spinal ganglion neurones. Neuroreport 9: 279-282.
95. Sakmann B, Neher E 1995. Single-Channel Recording. 2nd ed, Plenum Press, New York.
96. 7 receptors. Am J Physiol 275: F962-F971.
97. Seguela P, Haghighi A, Soghomonian JJ, Cooper E 1996. A novel neuronal P2x ATP receptor ion channel with widespread distribution in the brain. J Neurosci 16: 448-455.
98. Shibuya I, Tanaka K, Hattori Y, Uezono Y, Harayama N, Noguchi J, Ueta Y, Izumi, Yamashita H 1999. Evidence that multiple P2X purinoceptors are functionally expressed in rat supraoptic neurones. J Physiol 514: 351-367.
99. Simon J, Kidd EJ, Smith FM, Chessell IP, Murrell-Lagnado R, Humphrey PPA, Barnard EA 1997. Localization and functional expression of splice variants of the P2X2 receptor. Mol Pharmacol 52: 1-12.
100. Solini A, Chiozzi P, Morelli A, Fellin R, Virgilio FD 1999. Human primary fibroblasts in vitro express a purinergic P2X7 receptor coupled to ion fluxes, microvesicle formation and IL-6 release. J Cell Sci 112: 297-305.
101. Song SL, Chueh SH 1996. Antagonistic effect of Na+ and Mg2+ on P2z purinoceptor-associated pores in dibutyryl cyclic AMP-differentiated NG108-15 cells. J Neurochem 67: 1694-701.
102. Soto F, Garcia-Guzman M, Gomez-Hernandez JM, Hollmann M, Karschin C, Stuhmer W 1996a. P2X4: an ATP-activated ionotropic receptor cloned from rat brain. Proc Natl Acad Sci USA 93: 3684-3688.
103. Soto F, Garcia-Guzman M, Karschin C, Stuhmer W 1996b. Cloning and tissue distribution of a novel P2X receptor from rat brain. Biochem Biophys Res Commun 223: 456-460.
104. Steinberg TH, Newman AS, Swanson JA, Silverstein SC 1987. ATP4- permeabilizes the plasma membrane of mouse macrophages to fluorescent dyes. J Biol Chem 262: 8884-8888.
105. Sudo N, Tanaka K, Koga Y, Okumura Y, Kubo C, Nomoto K 1996. Extracellular ATP activates mast cells via a mechanism that is different from the activation induced by the cross-linking of Fc receptors. J Immunol 156: 3970-3979.
106. 1 purinoceptor in human platelets. J Biol Chem 273: 11544-11547.
107. 7). Science 272: 735-738.
108. Tatham PE, Lindau M 1990. ATP-induced pore formation in the plasma membrane of rat peritoneal mast cells. J Gen Physiol 95: 459-476.
109. Tenneti L, Gibbons SJ, Talamo Br 1998. Expression and trans-synaptic regulation of P2X4 and P2z receptors for extracellular ATP in parotid acinar cells. J Biol Chem 273: 26799-26808.
110. Ugur M, Drummond RM, Zou H, Sheng P, Singer JJ, Walsh Jr JV 1997. An ATP-gated cation channel with some P2z-like characteristics in smooth muscle cells of toad. J Physiol 498: 427-442.
111. Valera S, Hussy N, Evans RJ, Adami N, North RA, Surprenant A, Buell G 1994. A new class of ligand-gated ion channel defined by P2X receptor for extracellular ATP. Nature 317: 516-519.
112. Valera S, Talabot F, Evans RJ, Gos A, Antonarakis SE, Morris MA, Buell GN 1995. Characterization and chromosomal localization of a human P2X receptor from the urinary bladder. Receptors Channels 3: 283-289.
113. Virginio C, Church D, Alan North R, Surprenant A 1997. Effects of divalent cations, protons and calmidazolium at the rat P2X7 receptor. Neuropharmacology 36: 1285-1294.
114. Von Albertini M, Palmetshofer A, Kaczmarek E, Koziak K, Stroka D, Grey St, Stuhlmeier KM, Robson SC 1998. Extracellular ATP and ADP activate transcription factor NF-κβ and induce endothelial cell apoptosis. Biochem Biophys Res Commun 248: 822-829.
115. Walker BAM, Hagenlocker BE, Douglas VK, Tarapchak SJ, Ward PA 1991. Nucleotide responses of human neutrophils. Lab Invest 64: 105-112.
116. Wang CZ, Namba N, Gonoi T, Inagaki N, Seino S 1996. Cloning and pharmacological characterization of a fourth P2X receptor subtype widely expressed in brain and peripheral tissues including various endocrine tissues. Biochem Biophys Res Commun 220: 196-202.
117. + Uptake: Inhibition of Fluxes by Suramin. Arch Biochem Biophys 305: 54-60.
118. Wiley JL, Chen JR, Snook MB, Jamieson GP 1994. The P2z-purinoceptor of human lymphocytes: action of nucleotide agonists and irreversible inhibition by oxidized ATP. Br J Pharmacol 112: 946-950.
119. Wiley JL, Gargett CE, Zhang W, Snook MB, Jamieson GP 1998. Partial agonist and antagonists reveal a second permeability state of human lymphocyte P2z/ P2X7 channel. Am J Physiol 275: C1224-31.
120. Zoetewij JP, Van Der Water B, De Bont HJGM, Nagelkerke JF 1996. The role of a purinergic P2z receptor in calcium-dependent cell killing of isolated rat hepatocytes by extracellular adenosine triphosphate. Hepatology 23: 858-65.