Applications of Flow Cytometry to Hematopoietic Stem Cell Transplantation

Memorias do Instituto Oswaldo Cruz

Volumn 95, Issue 3, 2000, Pages 403-414

  Voltarelli, Júlio C ^a,b  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Bone marrow transplantation; Flow cytometry; Stem cell transplantation

Indexed keywords

ANIMALIA;

ANIMAL; CELL COUNT; CLASSIFICATION; DISEASE MODEL; FLOW CYTOMETRY; HEMATOLOGIC DISEASE; HEMATOPOIETIC STEM CELL; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMAN; LYMPHATIC SYSTEM DISEASE; METHODOLOGY; REVIEW;

ANIMALS; CELL COUNT; DISEASE MODELS, ANIMAL; FLOW CYTOMETRY; HEMATOLOGIC DISEASES; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HEMATOPOIETIC STEM CELLS; HUMANS; LYMPHATIC DISEASES;

EID: 0034181482     PISSN: 00740276     EISSN: None     Source Type: Journal    
DOI: 10.1590/S0074-02762000000300018     Document Type: Article

References (119)

1. Arkesteijn GJ, Erpelinck SL, Martens AC, Hagenbeek A 1995. Chromosome specific DNA hybridization in suspension for flow cytometric detection of chimerism in bone marrow transplantation. Cytometry 19: 353-360.
2. Atkinson TP, Bonitatibus GM, Berkov RL 1997. Chronic granulomatous disease in two children with recurrent infections: Family studies using dihydrorhodamine-based flow cytometry. J Pediatr 130: 488-491.
3. Ault KA, Antin JH, Ginsburg D, Orkin SH, Rappeport JM, Keohan ML, Martin P, Smith BR 1985. Phenotype of recovering lymphoid cell populations after marrow transplantation. J Exp Med 161: 1483-1502.
4. Baersch G, Mollers T, Hotte A, Dockhorn-Dworniczak B, Rube C, Ritter J, Jurgens H, Vormoor J 1997. Good engraftment of B cell precursors ALL in NOD-SCID mice. Klin Pediatr 209: 178-185.
5. Baum CM, Weissman IL, Tsukamoto AS, Buckle AM, Peault B 1992. Isolation of a candidate human hematopoietic stem cell population. Proc Natl Acad Sci 89: 2804-2808.
6. Bhatia M, Bonnet D, Murdoch B, Gan OI, Dick JE 1998. A newly discovered class of human hematopoietic cells with SCID-repopulating activity. Nat Med 4: 1038-1045.
7. Blanchard D, Bruneau V, Bernard D, Germond-Arnoult F, Gourbil A, David B, Muller JY 1995. Flow cytometric analysis of dual red cell populations after bone marrow transplantation. Br J Haematol 89: 741-747.
8. Bojko P, Hester JP, Durett AG, Maadani F, Korbling M, Champlin RE 1998. Identification of megacaryocyte precursors in peripheral blood stem cell collections from normal donors. J Clin Apheresis 13: 7-15.
9. Bomberger C, Singh-Jairam M, Rodey G, Guerriero A, Yeager AM, Fleming WH, Holland HK, Waller EK 1998. Lymphoid reconstitution after autologous PBSC transplantation with FACS-sorted CD34+ hematopoietic progenitors. Blood 19: 2588-2600.
10. Bonini C, Ferrari G, Verzeletti S, Savida P, Zappone E, Ruggieri L, Ponzoni M, Rossini S, Mavilio F, Traversari C, Bordignon C 1997. HSV-TK gene transfer into donor lymphocytes for control of allogeneic graft-versus-leukemia. Science 276: 1719-1724.
11. Brenner MK, Heslop HE, Rooney CM 1998. Gene and cell transfer for specific immunotherapy. Vox Sang 74 (Suppl. 2): 87-90.
12. Briones D, Urbano-Ispizua A, Orfao A, Marin P, Sierra J, Rovira M, Carreras E, Rozman C, Montserrat E 1998. Demonstration of donor origin of CD34+ HAL-DR- bone marrow cells after allogeneic peripheral blood transplantation with a long follow-up. Bone Marrow Transplant 21: 189-194.
13. Cairo MS, Wagner JE 1997. Placental and/or umbilical cord blood: na alternative source of hematopoietic stem cells for transplantation. Blood 90: 4665-4678.
14. Cassens U, Gutensonh K, Garritsen H, Kelsch R, Kuehnl P, Sibrowski W 1998. The influence of different erythrocyte lysing procedures on flow cytometric determination of CD34+ cells in umbilical cord blood transplants. Transfus Med 8: 111-118.
15. Castro FA, Paula Santos CM, Voltarelli JC 1999. Stimulation of HLA-DR antigen expression in acute and chronic leukemias. Cancer Research Therapy and Control, in press.
16. Cervero C, Escribano L, San Miguel JF, Diaz-Augustin B, Bravo P, Villarubia J, Garcia Sanz R, Velasco JL, Herrera P, Vargas M, Gonzalez M, Navarro JI, Orfao 1999. Expression of Bcl-2 by human bone marrow mast cells and its overexpression in mast cell leukemia. Am J Hematol 60: 191-195.
17. Chalmers IM, Janossy G, Contreras M, Navarette C 1998. Intracellular cytokine profile of cord and adult blood lymphocytes. Blood 92: 11-18.
18. Cheng L, Du C, Lavau C, Chen S, Tong J, Chen BP, Scollay R, Hawley RG, Hill B 1998. Sustained gene expression in retrovirally transduced, engrafting human hematopoietic stem cells and their lymphomyeloid progeny. Blood 92: 83-92.
19. Christianson SW, Greiner DL, Hesselton RA, Left JH, Wagar EJ, Schweitzer IB, Rajan TV, Gott B, Roopenian DC, Shultz LD 1997. Enhanced CD4+ T cell engraftment in beta2-microglobulin-deficient NOD SCID mice. J Immunol 158: 3578-3586.
20. Ciudad J, San Miguel JF, Lopez-Berges MC, Vidriales B, Valverde B, Ocqueteau M, Mateos G, Caballero MD, Hernandez J, Moro MJ, Mateos MV, Orfao A 1998. Prognostic value of immunophenotypic detection of minimal residual disease in acute lymphoblastic leukemia. J Clin Oncol 16: 3774-3781.
21. Cohen PL, Hoyer JD, Kurtin PJ, Dewald GW, Hanson CA 1998. Acute myeloid leukemia with minimal differentiation. A multiple parameter study. Am J Clin Pathol 109: 32-38.
22. Cotteret S, Belloc F, Boiron JM, Bilhou-Nabera C, Dumain P, Boyer C, Lacombe F, Reiffers J, Bernard P 1998. Fluorescent in situ hybridization on flow-sorted cells as a tool for evaluating minimal residual disease or chimerism after allogeneic bone marrow transplantation. Cytometry 34: 216-222.
23. Dansey R, Zielinsky I, Klein J, Karanes C, Abella S, Carrion R, Du W, Cassells L, Peters W, Baynes R 1998. Optimization of peripheral blood stem cell collections using peripheral blood CD34 levels and routine high volume apheresis. Blood 92 (Suppl. 1): 124a.
24. Dermime S, Mavroudis D, Jiang YZ, Hensel N. Molldrem J, Barrett AJ 1997. Immune scape from a graft-versus-leukemia effect may play a role in the relapse of myeloid leukemias following allogeneic bone marrow transplantation. Bone Marrow Transplant 19: 989-999.
25. Diamond HR, Souza MH, Bouzas LF, Tabak DG, Campos MM, Rumjanek VM 1995. Deficit of T-cell recovery after allogeneic bone marrow transplantation in chronic myeloid leukemia patients. Anticancer Res 15: 1553-1560.
26. Dimitriou H, Matsouka C, Perdikoyanni C, Stiakaki E, Bolonaki I, Lydaki E, Koumantakis E, Kalmanti M 1998. Phenotypic characteristics of cord blood hematopoietic cells. Leuk Res 22: 755-758.
27. Doukas MA, Fleming D, Jennings D 1998. Identical twin marrow transplantation for venous thrombosis in paroxismal nocturnal hemoglobinuria; long term complete remission as assessed by flow cytometry. Bone Marrow Transplant 22: 717-721.
28. Drenou B, Lamy T, Amiot L, Fardel O, Caulet-Maugendre S, Sasportes M, Diebold J, Le Prise PY, Fauchet R 1997. CD3-CD56+ non-Hogkin's lymphomas with an aggressive behaviour related to multidrug resistance. Blood 89: 2966-2974.
29. Dunbar PR, Ogg GS, Chen J, Rust N, van der Bruggen P, Cerundolo V 1998. Direct isolation, phenotyping and cloning of low frequency antigenic specific cytotoxic T lymphocytes from peripheral blood. Curr Biol 26: 413-416.
30. Epperson DE, Margolis DA, McOlash L, Janczah T, Barrett AJ 1999. T cells with graft-versus host (GVHD) or graft-versus-leukemia (GVL) reactivity can be identified by T cell receptor Vβ gene spectratyping. Tandem BMT Meeting, Keystone, CO, Abstract p. 38.
31. Falcão RP, Voltarelli JC, Pestana DR, Simoes BP, Zago MA, Figueiredo MS 1992. A T gamma/delta lymphoproliferative disease with NK activity. Am J Haematol 41: 128-131.
32. Farahat N, Morilla A, Owusu-Ankomah K, Morilla R, Pinkerton CR, Treleaven JG, Matutes E, Powles RL, Catovsky D 1998. Detection of minimal residual disease in B-lineage acute lymphoblastic leukaemia by quantitative flow cytometry. Br J Haematol 101: 158-164.
33. Fearnley LF, Whyte LF, Carnoutsos AS, Cook AH, Hart DNJ 1999. Monitoring human blood dendritic cell numbers in normal individuals and in stem cell transplantation. Blood 93: 728-736.
34. Fritsch G, Stimpfl M, Kurz M, Printz D, Buchinger P, Fischmeister G, Hoecker P, Gardner H 1996. The composition of CD34 subpopulations differ between bone marrow, blood and cord blood. Bone Marrow Transplant 17: 169-178.
35. Gan OI, Murdoch B, Larochelle A, Dick JE 1997. Differential maintenance of primitive human SCID-repopulating cells, clonogenic precursors, and long-term culture-initiating cells after incubation on human bone marrow stromal cells. Blood 90: 641-650.
36. Gebauer W, Fallgren-Gebauer E, Corbes C, Schunter F 1996. Quantitation of circulating hematopoietic progenitor cells early after transplantation. Beitr Infusionsther Transfusionsmed 33: 165-169.
37. Gluckman E, Rocha V, Chastang C for Eurocord-Cord Blood Transplant Group 1998. Cord Blood Hematopoietic Cells: Biology and Transplantation, Education Program Book, American Society of Hematology, Washington, DC, p. 1-14.
38. Gothot A, van der Loo JCM, Clapp DW, Srour EF 1998. Cell-cycle-related changes in repopulating capacity of human mobilized peripheral blood CD34+ cells in non-obese diabetic/severe combined immune-deficient mice. Blood 92: 2641-2649.
39. Greiner DL, Shultz LD, Yates J, Appel MC, Perdrizet G, Hesselton RM, Schwitzer I, Beamer WG, Shultz KL, Pelsue SC 1995. Improved engraftment of human spleen cells in NOD/LtSz-scid/scid mice as compared with CB-17-scid/scid mice. Am J Pathol 146: 888-902.
40. Greiner DL, Hesselton RA, Shultz LD 1998. SCID mouse models of human stem cell engraftment. Stem Cells 16: 166-177.
41. Greinix HT, Linkesch W, Keil F, Kahls P, Schwarzinger I, Schneider B, Oesterreicher C, Brugger S, Kapiotis S, Lechner K 1994. Early detection of hematopoietic engraftment after bone marrow and peripheral blood stem cell transplantation by highly fluorescent reticulocyte counts. Bone Marrow Transplant 14: 307-313.
42. Grimley M, Marmer D, Lee S, Filipovich A 1999. Phenotypic analysis of CD4 cells post stem cell transplant (SCT): Prolonged persistence of an activated phenotype and increased proportion of Th2 cells in recipients of matched unrelated donor (MUD) stem cell transplants (SCT). Tandem BMT Meetings, Keystone CO, Abstract p. 8.
43. Guenechea G, Segovia JC, Abella B, Lamana M, Ramirez M, Regidor C, Fernandez MN, Bueren JA 1999. Delayed engraftment of nonobese diabetic severe combined immunodeficient mice transplanted with ex vivo-expanded human CD34+ cord blood cells. Blood 93: 1097-1105.
44. Guillaume T, Rubinstein DB, Symann M 1998. Immune reconstitution and immunotherapy after autologous hematopoietic stem cell transplantation. Blood 92: 1471-1490.
45. Guthensonh K, Hummel K, Sputtek A, Cassens U, Kuhnl P 1996. Storage of peripheral blood cell samples alters flow cytometric CD34+ results. Beitr Infusionsther Transfusionsmed 33: 170-174.
46. Heitger A, Neu N, Kern H, Panzer-Grumayer ER, Greinix H, Nachbaur D, Niederwieser D, Fink FM 1997. Essential role of the thymus to reconstitute naive (CD45RA+) T-helper cells after human allogeneic bone marrow transplantation. Blood 90: 850-857.
47. Hendriks EC, de Man AJ, van Berkel IC, Stienstra S, de Witte T 1997. Flow cytometric method for the routine follow-up of red cell populations after bone marrow transplantation. Br J Haematol 97: 141-145.
48. Hogan CJ, Shpall EJ, McNulty O. 1997. Engraftment and development of human CD34+ enriched cells from umbilical cord blood in NOD/LtSz-scid/scid mice. Blood 90: 85-96.
49. Honda J, Okubo Y, Iamamura Y, Kusaba M, Saruwatari M, Oizumi K 1997. Flow cytometric detection of cytomegalovirus antigen in peripheral blood cells after bone marrow transplantation. Br J Haematol 99: 415-417.
50. Imbert-Marcille B-M, Robillard M, Poirier A-S, Coste-Burel M, Cantarovich D, Milpied N, Billaudel S 1997. Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry. J Clin Microbiol 35: 2665-2669.
51. Jennings CD, Foon KA 1997. Recent advances in flow cytometry: applications to the diagnosis of hematologic malignancy. Blood 90: 2863-92.
52. Johnsen HE, Rasmussen T, Knudsen LM 1998. CD34+ subset and tumor cell quantitation by flow cytometry-step toward quality assessment of autografts in B cell malignancies. Vox Sang 74 (Suppl. 2): 477-482.
53. Klein C, Cavazzana-Calvo M, Le Deist F, Jabado N, Benkerrou M, Blanche S, Lisowska-Grospierre B, Griscelli C, Fischer A 1995. Bone marrow transplantation in major histocompatibility complex class II deficiency: a single center study of 19 patients. Blood 85: 580-587.
54. Kohler M, Dittman J, Legler TJ, Lynen R, Humpe A, Riggert J, Neumeyer H, Pies A, Panzer S, Mayr WR 1996. Flow cytometric detection of platelet-reactive antibodies and application in platelet crossmatching. Transfusion 36: 250-255.
55. Konikova E, Glasova M, Kusenda J, Babusikova O 1998. Intracellular markers in acute myeloid leukemia diagnosis. Neoplasma 45: 282-291.
56. Korbling M, Drach J, Champlin RE, Engel H, Huynh L, Kleine HD, Berenson R, Deisseroth AB, Andreeff M 1994. Large-scale preparation of highly purified, frozen/thawed CD34+, HLA-DR- hematopoietic progenitor cells by sequential immunoadsorption (CEPRATE-SC) and fluorescence-activated cell sorting: implications for gene transduction and/or transplantation. Bone Marrow Transplant 13: 649-654.
57. Korbling M, Huh YO, Durett A, Mirza N, Miller P, Engel H, Anderlini P, van Besien K, Andreeff M, Przepiorka D, Deisseroth AB, Champlin RE 1995. Allogeneic blood stem cell transplantation: peripherialization and yield of donor-derived primitive hematopoietic progenitor cells (CD34+Thy-1-dim) and lymphoid subsets, and possible predictors of engraftment and graft-versus-host disease. Blood 86: 2842-2848.
58. Lacerda JF, Ladanyi M, Louie DC, Fernandez JM, Papadopoulos EB, O'Reilly RJ 1996. Human Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes home preferentially and induce selective regressions of autologous EBV-induced B cell lymphoproliferation in xenografted CB-17 scid/scid mice. J Exp Med 183: 1215-1228.
59. Lapidot T, Pflumio F, Doedens M, Murdoch B, Williams DE, Dick JE 1992. Cytokine stimulation of multilineage hematopoiesis from immature human cells engrafted in SCID mice. Science 255: 1137-1141.
60. Larochelle A, Vormoor J, Hanenberg H, Wang JC, Bhatia M, Lapidot T, Moritz T, Murdoch B, Xiao XL, Kato I, Williams DA, Dick JE 1996. Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: implications for gene therapy. Nat Med 2: 1329-1337.
61. Leuner S, Arland M, Kahl C, Jentsch-Ulrich K, Franke A, Hofkes HG 1998. Enumeration of CD34-positive hematopoietic progenitor cells by flow cytometry: comparison of a volumetric assay and the ISHAGE gating strategy. Bone Marrow Transplant 22: 699-706.
62. Lum LG 1990. Immune recovery after bone marrow transplantation. Hematol Oncol Clin North Am 4: 659-675.
63. Macedo A, Orfao A, Martinez Z, Vidriales MB, Valverde B, Lopez-Berges MC, San Miguel JF 1995. Immunophenotype of c-kit cells in normal human bone marrow: implications for the detection of minimal residual disease in AML. Br J Haematol 89: 338-341.
64. Macey MG, McCarthy DA, van Aghtoven A, Newland AC 1997. How should CD34+ cells be analysed? A study of three classes of antibody and five leucocyte preparation procedures. J Immunol Methods 204: 175-188.
65. Madrigal JA, Cohen SBA, Gluckman E, Charron DJ 1997. Does cord blood transplantation result in lower graft versus host disease? It takes more than two to tango. Human Immunol 56: 1-5.
66. Maher GM, Hartman KR 1993. Detection of antineutrophil antibodies by flow cytometry: use of unfixed neutrophils as antigenic targets. J Clin Lab Anal 7: 334-340.
67. Martin PJ, Hansen JA, Thomas ED 1984. Use of flow microfluorometry in bone marrow transplantation. Ann NY Acad Sci 428: 14-25.
68. Matsumoto T, Anamoto N, Kondoh T, Kakayama N, Takayanagi T, Tsuji Y 1998. Complete DiGeorge syndrome treated by bone marrow transplantation. Bone Marrow Transplant 22: 927-930.
69. Mavroudis D, Read E, Cottler-Fox M, Couriel D, Molldrem J, Carter C, Yu M, Dunbar C, Barrett J 1996. CD34+ cell dose predicts survival, posttransplant morbidity, and rate of hematologic recovery after allogeneic marrow transplants for hematologic malignancies. Blood 88: 3223-3229.
70. McCune JM, Namikawa R, Kaneshima H, Shultz LD, Lieberman M, Weissman IL 1988. The SCID-hu mouse: murine model for the analysis of human hematolymphoid differentiation and function. Science 241: 1632-1639.
71. Menendez P, Redondo O, Rodriguez A, Lopez-Berges MC, Ercilia G, Lopez A, Duran A, Almeida J, Perz-Simon JA, San Miguel JF, Gratama JW, Orfao A 1998. Comparison between lyse-and-then-wash and a lyse-non-wash technique for the enumeration of CD34+ hematopoietic progenitor cells. Cytometry 34: 264-271.
72. Mosier DE, Gulizia RJ, Baird SM, Wilson DB 1988. Transfer of functional human immune system to mice with severe combined immunodeficiency. Nature 335: 256-259.
73. Miyoshi H, Smith KA, Mosier DE, Verma IM, Torbett BE 1999. HIV vectors efficiently transduce human CD34+ cells that mediate long-term engraftment of NOD/SCID mice. Science 283: 682-686.
74. Nelson M, Popp H, Forsyth C, Gibson J 1996. Rapid quantitation of mixed red cell populations using flow cytometry. Clin Lab Haematol 18: 207-213.
75. Nijmeier BA, Willemze R, Falkenburg JHF 1998. An animal model for human adoptive immunotherapy: in vivo elimination of human leukemia by cytotoxic effector cells in NOD/SCID mice. Blood 92 (Suppl. 1): 437a.
76. Ocqueteau M, Orfao A, Garcia R, San Miguel JF 1996. Detection of monoclonality in bone marrow plasma cells by flow cytometry: limitations for minimal residual disease detection. Br J Haematol 93: 251-252.
77. Olivero S, Alario T, Ladaique P, Haccoun M, Viens P, Blaise D, Chabannon C 1999. CD34+ cell enumeration in peripheral blood and apheresis samples, using two laboratoriy diagnostic kits or na institutional protocol. Bone Marrow Transplant 23: 387-394.
78. Orfao A, Garcia-Sanz R, Lopez-Berges MS, Belen-Vidriales M, Gonzalez M, Caballero MD, San Miguel JF 1994. A new method for the analysis of plasma cell DNA content in multiple myeloma samples using a CD38/propidium iodide double staining technique. Cytometry 17: 332-339.
79. Peled A, Petit I, Kollet O, Magid M, Ponomaryov T, Byk T, Nagler A, Bem-Hur H, Many A, Shultz L, Lider O, Alon R, Zipori D, Lapidot T 1999. Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4. Science 283: 845-848.
80. Perez-Simon JA, Caballero MD, Corrall M, Nieto MJ, Orfao A, Vasquez L, Amigo ML, Berges C, Gonzalez C, Del Canizzo C, San Miguel JF 1998. Minimal number of circulating CD34+ cells to ensure successfull leukapheresis and engraftment in autologous peripheral blood progenitor cell transplantation. Transfusion 38: 385-391.
81. Pflumio F, Izac B, Katz A, Schultz LD, Vainchenker W, Coulombel L 1996. Phenotype and function of human hematopoietic cells engrafting immune-deficient CB17-severe combined immunodeficiency mice and non-obese diabetic-severe combined immunodeficiency mice after transplantation of human cord blood mononuclear cells. Blood 88: 3731-3740.
82. Rasmussen T, Honore L, Johnsen HE 1998. Identification and characterisation of malignant cells using TR-PCR on single flow-sorted cells. Med Oncol 15: 96-102.
83. Rebouisoou C, Wijdenes J, Autissier P, Tarte K, Costes V, Liautard J, Rossi J-F, Brochier J, Klein B 1998. A gp130 interleukin-6 transducer-dependent SCID model of human multiple myeloma. Blood 91: 4727-4737.
84. Rego EM, Garcia AB, Viana SR, Falcão, RP. 1996. Characterization of acute lymphoblastic leukemia subtypes in Brazilian patients. Leukemia Res 20: 349-355.
85. Rego EM, Garcia AB, Viana SR, Falcão RP 1998a. Age-related changes of lymphocyte subsets in normal bone marrow biopsies. Cytometry 34: 22-29.
86. Rego EM, Garcia AB, Falcão RP 1998b. Combined quantification of CD79a, TdT, CD34, CD19 and CD10 densities by quantimetry distinguishes between normal and leukemic B-precursors in the bone marrow. Blood 92 (Suppl. 1): 229a.
87. Rego EM, Tone LG, Garcia AB, Falcao RP 1999. CD10 and CD19 fluorescence intensity of B cell precursors in normal and leukemic bone marrow. Clinical characterization of CD10+ strong and CD10+ weak common acute lymphoblastic leukemia. Leukemia Res 23: 431-440.
88. Richardson C, Bank A 1995. Preselection of transduced murine hematopoietic stem cell populations leads to increased long-term stability and expression of the human multiple drug resistance gene. Blood 86: 2579-2589.
89. Richter J 1997. Gene transfer to hematopoietic cells- the clinical experience. Eur J Haematol 69: 67-75.
90. Rill DR, Holliday M, Heslop HE, Krance RA, Kimbrough S, Klingemann H, Brenner MK 1997. Long term transgene expression by human hematopoietic cells in vitro. Blood 90 (Suppl. 1): 405
91. Rumi C, Rutella S, Bonini S, Bonini S, Lambiase A, Sica S, Puggioni P, Salutari P, Etuk B, Leone G 1998. Immunophenotypic profile of peripheral blood eosinophils in acute graft-vs-host disease. Exp Hematol 26: 170-178.
92. Rybka WB, Fontes PA, Rao AS, Winkelstein A, Ricordi C, Ball ED, Starzl TE 1995. Hematopoietic stem cell content of vertebral body marrow used for combined solid organ transplantation. Transplantation 59: 871-874.
93. San Miguel JF, Hernandez MD, Gonzalez M, Lopez-Berges MC, Caballero MD, Vazquez L, Orfao A, Nieto Mj, Corral M, del Canizo MC 1996. A randomized study comparing the effect of GM-CSF and G-CSF on immune recontitution after autologous bone marrow transplantation. Br J Haematol 94: 140-147.
94. San Miguel JF, Martinez A, Macedo A, Vidriales MB, Lopez-Berges C, Gonzalez M, Caballero D, Garcia-Marcos MA, Ramos F, Fernandez-Calvo J, Calmuntia MJ, Diaz-Mediavilla J, Orfao 1997. Immunophenotyping investigation of minimal residual disease is a useful approach for predicting relapse in acute myeloid leukemia patients. Blood 90: 2465-2470.
95. Sasaki DT, Tichenor EH, Lopez F, Combs J, Uchida N, Smith CR, Stokdijk W, Vardanega M, Buckle AM, Chen B. 1995. Development of a clinically applicable high-speed flow cytometer for the isolation of tranplantable human hematopoietic stem cells. J Hemathoter 4: 503-514.
96. Schots R, van Riet I, Damiaens S, Flament J, Lacor P, Staelens Y, Steenssens L, van Camp B, DeWaele M 1996. The absolute number of circulating CD34+ cells predicts the number of hematopoietic stem cells that can be collected by apheresis. Bone Marrow Transplant 17: 509-515.
97. Small TN, Papadopoulos EB, Boulad F, Black P, Castro-Malaspina H, Childs BH, Collins N, Gillio A, George D, Jakubowski A, Heller G, Fazzari M, Kernan N, MacKinnon S, Szabolcs P, Young JW, O'Reilly RJ 1999. Comparison of immune reconstitution after unrelated and related T-cell depleted bone marrow transplantation: effect of patient age and donor leukocyte infusions. Blood 93: 467-480.
98. SMSGH- Spanish Multicentric Study Group for Hematopoietic Recovery 1994. Flow cytometric reticulocyte quantification in the evaluation of hematologic recovery. Eur J Haematol 53: 293-297.
99. Souza MH, Diamond HR, Silva ML, Campos MM, Bouzas LF, Tabak D, Flowers ME, Rumjanek VM 1994. Immunological recovery after bone marrow transplantation for severe aplastic anemia: a Brazilian experience. Eur J Haematol 53: 150-155.
100. Suzuki R, Yamamoto K, Seto M, Kagami Y, Ogura M, Yatabe Y, Suchi T, Kodera Y, Morishima Y, Takahashi T, Saito H, Ueda R, Nakamura S 1997. CD7+ and CD56+ myeloid/natural killer cell precursor acute leukemia: a distinct hematolymphoid disease entity. Blood 90: 2417-2428.
101. Tabernero MD, San Miguel JF, Garcia JL, Garcia-Isidoro M, Wiegant J, Ciudad J, Gonzalez M, Rios A, Raap A, Orfao A 1995. Clinical, biological, and immunophenotypical characteristics of B-cell chronic lymphocytic leukemia with trisomy 12 by fluorescence in situ hybridization. Cytometry 22: 217-222.
102. Talmadge JE, Reed E, Ino K, Kessinger A, Kuszynski C, Heimann D, Varney M, Jackson J, Vose JM, Bierman PJ 1997. Rapid immunologic reconstitution following transplantation with mobilized peripheral blood stem cells as compared to bone marrow. Bone Marrow Transplant 19: 161-72.
103. Tarantolo SR, Bishop MR, Lynch JC, Davis JR, Pirruccello SJ, Bierman PJ, Pavletic ZS, Armitage JO, Jessinger A, Gross TG 1998. Effect of G-CSF mobilization on type 1 and type 2 T-cells and correlation to acute graft-versus-host disease (GvHD). Blood 92(Suppl. 1): 722a.
104. Thomas C, Le Deist F, Cavazzana-Calvo M, Benkerrou M, Haddad E, Blanche S, Hartman W, Friedrich W, Fischer A 1995. Results of allogeneic bone marrow transplantation in patients with leukocyte adhesion deficiency. Blood 86: 1629-1635.
105. Thomas ED 1994. The Nobel Lectures in Immunology. The Nobel Prize for Physiology or Medicine, 1990. Bone Marrow Transplantation- past, present and future. Scand J Immunol 39: 339-45.
106. Trenschel R, Bernier M, Delforge A, Massy M, Lebeau DHE, Maerevoet M, Badjou R, Stryckmans P, Bron D 1998. Myeloid and lymphoid recovery following allogeneic bone marrow transplantation: a comparative study between related, unrelated bone marrow and allogeneic peripheral stem cell transplantation. Leuk Lymphoma 30: 325-352.
107. Uchida N, Coombs J, Chen S, Zanjani E, Hoffman R, Tsukamoto A 1996. Primitive human hematopoietic cells displaying differential eflux of the rhodamine 123 dye have distinct biologic activities. Blood 88: 1297-1305.
108. Uckun FM 1996. Severe combined immunodeficient mouse models of human leukemia. Blood 88: 1135-1146.
109. Van der Loo JCM, Hanenberg H, Cooper RJ, Luo F-Y, Lazaridis EN, Williams DA 1998. Nonobese diabetic/severe combined immunodeficiency (NOD/ SCID) mouse as a model system to study the engraftment and mobilization of human peripheral blood stem cells. Blood 92: 2556-2570.
110. Van Tol MJ, Langlois van den Bergh R, Mesker W, Ouwerkerk-van Velzen MC, Vossen JM, Tanke HJ 1998. Simultaneous detection of X and Y chromosomes by two-colour fluorescence in situ hybridization in combination with immunophenotyping of single cells to document chimaerism after sex-mismatched bone marrow transplantation. Bone Marrow Transplant 21: 497-503.
111. Vigorito AC, Azevedo WM, Marques JFC, Azevedo AM, Eid KAB, Aranha FJP, Lorand-Metze I, Oliveira GB, Correa MEP, Reis ARC, Miranda ECM, Souza CA 1998. A randomized, prospective comparison of allogeneic bone marrow and peripheral blood progenitor cell transplantation in the treatment of hematological malignancies. Bone Marrow Transplant 22: 1145-1151.
112. Voltarelli JC, Stites DP 1986. Immunological monitoring of bone marrow transplantation. Diagn Immunol 4: 171-193.
113. Voltarelli JC, Przepiorka D, Sandell ML, Johnson GA, Kopecky K, Martin PJ, Torok-Storb B 1989. CD8+/ DR+/CD25- T lymphocytes associated with marrow graft failure. Bone Marrow Transplant 4: 647-652.
114. Voltarelli JC, Corpuz S, Martin PJ 1990. In vitro comparison of two methods of T cell depletion associated with different rates of graft failure after allogeneic marrow transplantation. Bone Marrow Transplant 6: 419-423.
115. Warren EH, Bonnet D, Greenberg PD, Dick JE, Riddell SR 1997. CD8+ minor histocompatibility antigen-specific CTL prevent AML engraftment in NOD/ SCID mice. Blood 90 (Suppl. 1): 395a
116. Wells DA, Hall MC, Shulman HM, Loken MR 1998a. Occult B cell malignancies can be detected by three-color flow cytometry in patients with cytopenia. Leukemia 12: 2015-23.
117. Wells DA, Sale GE, Shulman HM, Myerson D, Bryant EM, Gooley T, Loken MR 1998b. Multidimensional flow cytometry can differentiate leukemic from normal lymphoblasts and myeloblasts after chemotherapy and bone marrow transplantation. Am J Clin Pathol 110: 84-94.
118. Yamada M, Ohtsu M, Kobayashi I, Kawamura N, Kobayashi K, Ariga T, Sakiyama Y, Nelson DL, Tsuruta S, Anakura M, Ishikawa M 1999. Flow cytometric analysis of Wiskott-Aldrich syndrome (WAS) protein in lymphocytes from WAS patients and their family carriers. Blood 93: 756-759.
119. Yurasov S, Kollman TR, Kim A, Raker CA, Hachamovitch M, Wong-Staal F, Goldstein H 1997. Severe combined immunodeficient mice engrafted with human T cells, B cells and myeloid cells after transplantation with human fetal bone marrow or liver cells and implanted with human fetal thymus: a model for studying human gene therapy. Blood 89: 1800-1810.