Transduction of human CD34+ cells that mediate long-term engraftment of NOD/SCID mice by HIV vectors

Science

Volumn 283, Issue 5402, 1999, Pages 682-686

  Miyoshi, Hiroyuki ^a   Smith, Kent A ^b   Mosier, Donald E ^b   Verma, Inder M ^a   Torbett, Bruce E ^b  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD34 ANTIGEN; CYTOKINE; VIRUS VECTOR;

ARTICLE; CYTOKINE RELEASE; GENE THERAPY; GENE TRANSFER; GENETIC TRANSDUCTION; HEMATOPOIESIS; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS; MOUSE; NONHUMAN; PRIORITY JOURNAL; T LYMPHOCYTE; TRANSGENE;

ANIMALS; ANTIGENS, CD34; BONE MARROW CELLS; CELL DIVISION; CELL SURVIVAL; COLONY-FORMING UNITS ASSAY; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HEMATOPOIESIS; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HEMATOPOIETIC STEM CELLS; HIV; HUMANS; LEUKEMIA VIRUS, MURINE; LUMINESCENT PROTEINS; MICE; MICE, INBRED NOD; MICE, SCID; PROMOTER REGIONS (GENETICS); TRANSFECTION; TRANSGENES;

HUMAN IMMUNODEFICIENCY VIRUS;

EID: 0033613827     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.283.5402.682     Document Type: Article

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45. We thank S. Tam and the Labor and Delivery nursing staff at the Scripps Memorial Hospital for their efforts in providing cord blood. We also thank H. Perkin and B. Griffeth for technical assistance, N. Somia for critical reading of the manuscript, and members of the Verma and Torbett laboratories for helpful discussions. H.M. was supported by a fellowship from the Uehara Memorial Foundation. This work was supported by NIH grants CA44360 and HL53670 (I.M.V.) and DK49886 (B.E.T.), the March of Dimes Foundation (I.M.V.), and the Frances Berger Foundation (I.M.V.). I.M.V. is a Franklin D. Roosevelt Fellow of the March of Dimes Foundation and an American Cancer Society Professor of Molecular Biology. This is publication 11955-IMM from the Department of Immunology, the Scripps Research Institute.