Integrated bioprocess for production of human proinsulin C-peptide via heat release of an intracellular heptameric fusion protein

Journal of Biotechnology

Volumn 76, Issue 2-3, 2000, Pages 215-226

  Jonasson, Per ^a   Nygren, Per Åke ^a   Jörnvall, Hans ^b   Johansson, Bo Lennart ^c   Wahren, John ^c   Uhlén, Mathias ^a   Ståhl, Stefan ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

^c KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

C peptide; Enzymatic cleavage; Escherichia coli; Fusion protein; Heat release; Multimerization

Indexed keywords

CELL CULTURE; CHROMATOGRAPHY; ENZYMES; ESCHERICHIA COLI; GENES; INSULIN; NUCLEIC ACIDS; PROTEINS;

ENZYMATIC CLEAVAGE; FUSION PROTEINS; HEAT RELEASE; MULTIMERIZATION; PEPTIDE;

BIOTECHNOLOGY;

ARGININE; C PEPTIDE; CATION; CELL PROTEIN; GENE PRODUCT; HYBRID PROTEIN; MONOMER; NUCLEIC ACID; POLYELECTROLYTE; POLYMER; PROINSULIN; RECOMBINANT PROTEIN;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BIOPROCESS; CONTROLLED STUDY; CULTURE MEDIUM; CYTOPLASM; DRUG MANUFACTURE; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; HEAT TREATMENT; HOST CELL; NONHUMAN; PRECIPITATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVERSED PHASE LIQUID CHROMATOGRAPHY;

BIOREACTORS; BIOTECHNOLOGY; C-PEPTIDE; CARBOXYPEPTIDASE B; CARBOXYPEPTIDASES; CHROMATOGRAPHY, LIQUID; HEAT; HUMANS; NUCLEIC ACIDS; PRECIPITATION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; TRYPSIN;

ESCHERICHIA COLI;

EID: 0033990161     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(99)00195-9     Document Type: Article

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