Purification of recombinant apolipoprotein A-1(Milano) expressed in Escherichia coli using aqueous two-phase extraction followed by temperature- induced phase separation

Journal of Chromatography B: Biomedical Applications

Volumn 711, Issue 1-2, 1998, Pages 97-109

  Persson, Josefine ^a   Nyström, Lena ^b   Ageland, Hans ^b   Tjerneld, Folke ^a  

^a LUND UNIVERSITY   (Sweden)

^b PFIZER INC   (United States)

Author keywords

Apolipoprotein A 1; Aqueous two phase systems; Recombinant proteins; Thermo separating polymer

Indexed keywords

APOLIPOPROTEIN A1; COPOLYMER; DNA; ETHYLENE OXIDE; PROPYLENE OXIDE; RECOMBINANT PROTEIN; STARCH; UREA;

AQUEOUS SOLUTION; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PH; PHASE SEPARATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TEMPERATURE;

APOLIPOPROTEIN A-I; CHEMISTRY, ANALYTICAL; CLONING, MOLECULAR; DNA, RECOMBINANT; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; IONS; POLYETHYLENES; POLYPROPYLENES; RECOMBINANT PROTEINS; SALTS; TEMPERATURE; UREA;

EID: 0032568988     PISSN: 15726495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-4347(98)00029-2     Document Type: Article

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