Expanded bed adsorption at production scale: Scale-up verification, process example and sanitization of column and adsorbent

Bioprocess Engineering

Volumn 16, Issue 2, 1997, Pages 57-63

  Barnfield Frej, A K ^a   Johansson, H J ^a   Johansson, S ^a   Leijon, P ^a  

^a GE Healthcare Bio Sciences AB   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

RECOMBINANT PROTEIN;

ARTICLE; BIOREACTOR; DRUG MANUFACTURE; PRIORITY JOURNAL; TECHNIQUE;

EID: 0031013739     PISSN: 0178515X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004490050288     Document Type: Article

References (11)

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2. Batt, B.C.; Yabannavar, V.M.; Singh, V.: Expanded bed adsorption process for protein recovery from whole mammalian cell culture broth. Bioseparation 5 (1995) 41-52
3. Chang, Y.K.; Chase, H.A.: Development of operating conditions for protein purification using expanded bed techniques: The effect of the degree of bed expansion on adsorption performance. Biotechn. Bioeng. 49 (1996) 512-526
4. Chang, Y.K.; McCreath, G.E.; Chase, H.A.: Development of an expanded bed technique for an affinity purification of G6PDH from unclarified yeast cell homogenates. Biotechn. Bioeng. 48 (1995) 355-366
5. Chase, H.A.: Purification of proteins by adsorption chromatography in expanded beds. TIBTECH 12 (1994) 296-303
6. Hansson, M.; Ståhl, S.; Hjorth, R.; Uhlén, M.; Moks, T.: Single-step recovery of a secreted recombinant protein by expanded bed adsorption. Bio/Technol. 12 (1994) 285-288
7. Hjorth, R.; Kämpe, S., Carlsson, M.: Analysis of some operating parameters of novel adsorbents for recovery of proteins in expanded beds. Bioseparation 5 (1995) 217-223
8. Levenspiel, O.: Nonideal flow. In: Chemical Reaction Engineering, 2nd Ed., pp. 253-295. New York: John Wiley & Sons 1972
9. Lindgren, A.; Johansson, S.; Nyström, L.-E.: Scale up of expanded bed adsorption. In: Henon, B. (Ed.): The Seventh Bioprocess Engineering Symposium, BED-vol. 27, book no. H00887, pp. 27-30. The American Society of Mechanical Engineers 1993
10. Noppe, W.; Hanssens, I.; De Cuyper, M.: Simple two-step procedure for the preparation of highly active pure equine milk lysozyme. J. Chromatogr. A 719 (1996) 327-331
11. Thömmes, J.; Halfar, M.; Lenz, S.; Kula, M.-R.: Purification of monoclonal antibodies from whole hybridoma fermentation broth by fluidized bed adsorption. Biotechn. Bioeng. 45 (1995) 205-211