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0007054556
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of special interest. This study shows the usefulness of charged polymers in combination with low-cost polymers in extractive fermentations with bacteria. Charged polymers can be separated from the product extracting phase by phase separation using multivalent ions.
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Kwon YJ, Kaul R, Mattiasson B. Extractive lactic acid fermentation in poly(ethyleneimine)-based aqueous two-phase system. of special interest Biotechnol Bioeng. 50:1996;280-290 This study shows the usefulness of charged polymers in combination with low-cost polymers in extractive fermentations with bacteria. Charged polymers can be separated from the product extracting phase by phase separation using multivalent ions.
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Kwon, Y.J.1
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0029742390
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of special interest. This study shows the usefulness of EO - PO polymers in extractive fermentations with bacteria. The EO - PO polymers can be separated from the product extracting phase by a temperature shift.
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Planas J, Radstrom P, Tjerneld F, Hahn-Hagerdal B. Enhanced production of lactic acid through the use of a novel aqueous two-phase system as an extractive fermentation system. of special interest Appl Microbiol Biotechnol. 45:1996;737-743 This study shows the usefulness of EO - PO polymers in extractive fermentations with bacteria. The EO - PO polymers can be separated from the product extracting phase by a temperature shift.
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Planas, J.1
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0342875533
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T.G. II Pretlow, Pretlow ThP II New York: Academic Press
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Walter H. Separation and subfractionation of blood cell populations based on their surface properties by partitioning in two-polymer aqueous phase systems. Pretlow TG II, Pretlow ThP II. Cell Separation: Methods and Selected Applications. 1:1982;261-299 Academic Press, New York.
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Park KM, Wang NS. α-amylase fermentation with Bacillus amyloliquefaciens in an aqueous two-phase system. Biotechnol Prog. 7:1991;439-444.
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Chen, J.P.1
Lee, M.S.2
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0030131706
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of outstanding interest. This study uses a statistical experimental design approach to obtain an empiricial model for the partitioning of cells. These models are of paramount importance for improved process control of extractive fermentations.
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Zijlstra GM, Michielsen M, De Gooijer CD, Van der Pol LA, Tramper J. Hybridoma and CHO cell partitioning in aqueous two-phase systems. of outstanding interest Biotechnol Prog. 12:1996;363-370 This study uses a statistical experimental design approach to obtain an empiricial model for the partitioning of cells. These models are of paramount importance for improved process control of extractive fermentations.
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Zijlstra, G.M.1
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19
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0028821075
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Mukataka S, Haynes CA, Prausnitz JM, Blanch HW. Extractive bioconversions in aqueous two-phase systems: enzymatic hydrolysis of casein proteins. Biotechnol Bioeng. 40:1992;195-206.
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23
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0343145697
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Analysis of phase composition in aqueous two-phase systems using a two-column chromatographic method: Application to lactic acid production by extractive fermentation
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Planas J, Lefebvre D, Tjerneld F, Hahn-Hägerdahl B. Analysis of phase composition in aqueous two-phase systems using a two-column chromatographic method: application to lactic acid production by extractive fermentation. of outstanding interest Biotechnol Bioeng. 54:1997;303-311 This study unambiguously shows that drifts in phase compositions occur during extractive fermentations. In order to maintain a steady state during continuous processing, sophisticated process control is necessary. The on line measurement method described is an important tool in process control.
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Liakopoulou-Kyriakides M, Karakatsanis A, Stamatoudis M. Enzymic hydrolysis of starch in agitated PEG-dextran aqueous two-phase system. Starch. 48:1996;291-294.
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Min KH, Chang WJ, Koo YM. Increased production of beta-cyclodextrin using an aqueous two-phase system. Biotechnol Tech. 10:1996;395-400.
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Continuous hydrogen production by Clostridium sp. strain no. 2 from cellulose hydrolysate in an aqueous two-phase system
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Persson I, Staalbrand H, Tjerneld F, Hahn-hägerdal B. Semicontinuous production of cellulolytic enzymes with Trichoderma reesei Rutgers C30 in an aqueous two-phase system. Appl Biochem Biotechnol. 27:1992;27-36.
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Persson I, Tjerneld F, Hahn-haegerdal B. Influence of cultivation conditions on the production of cellulolytic enzymes with Trichoderma reseei Rutgers C30 in aqueous two-phase systems. Appl Biochem Biotechnol. 27:1992;9-25.
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Kuboi R, Umakoshi H, Komasawa I. Extractive cultivation of Escherichia coli using poly(ethylene glycol)/phosphate aqueous two-phase systems to produce intracellular β-galactosidase. Biotechnol Prog. 11:1995;202-207.
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Komasawa, I.3
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35
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0030040002
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Extractive cultivation of recombinant Escherichia coli using aqueous two-phase systems for production and separation of intracellular heat shock proteins
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of outstanding interest. This study demonstrates the feasibility of producing high-value recombinant proteins from E. coli by means of extractive fermentation in ATPSs. A semi-continuous production scheme is proposed, to allow periodical cell disruption and product release.
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Umakoshi H, Yano K, Kuboi R, Komasawa I. Extractive cultivation of recombinant Escherichia coli using aqueous two-phase systems for production and separation of intracellular heat shock proteins. of outstanding interest Biotechnol Prog. 12:1996;51-56 This study demonstrates the feasibility of producing high-value recombinant proteins from E. coli by means of extractive fermentation in ATPSs. A semi-continuous production scheme is proposed, to allow periodical cell disruption and product release.
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Biotechnol Prog
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Umakoshi, H.1
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Komasawa, I.4
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36
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0030199580
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Design of aqueous two-phase systems supporting animal cell growth: A first step towards extractive bioconversions
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of outstanding interest. This study demonstrates that the principle of extractive fermentation in ATPSs can also be applied to animal cells. The benefits of extractive fermentations, continuous operation with scalable cell retention, in situ product removal and integration of downstream processing with fermentation, now become available for animal cell cultivation.
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Zijlstra GM, de Gooijer CD, Van der Pol LA, Tramper J. Design of aqueous two-phase systems supporting animal cell growth: a first step towards extractive bioconversions. of outstanding interest Enz Microb Technol. 19:1996;2-8 This study demonstrates that the principle of extractive fermentation in ATPSs can also be applied to animal cells. The benefits of extractive fermentations, continuous operation with scalable cell retention, in situ product removal and integration of downstream processing with fermentation, now become available for animal cell cultivation.
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Enz Microb Technol
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Zijlstra, G.M.1
De Gooijer, C.D.2
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Use of multifactorial analysis to develop aqueous two-phase systems for isolation of non-native IgF1
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Hart RA, Ogez JR, Builder SE. Use of multifactorial analysis to develop aqueous two-phase systems for isolation of non-native IgF1. Bioseparation. 5:1995;113-121.
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Hart, R.A.1
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Builder, S.E.3
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39
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0030436455
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Separation of hybridoma cells from their IgG product using aqueous two-phase systems
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of special interest. This study uses a statistical experimental design to generate data for the modeling of product partitioning.
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Zijlstra GM, Michielsen MJF, de Gooijer CD, Van der Pol LA, Tramper J. Separation of hybridoma cells from their IgG product using aqueous two-phase systems. of special interest Bioseparation. 6:1996;201-210 This study uses a statistical experimental design to generate data for the modeling of product partitioning.
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Tramper, J.5
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40
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of special interest
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Zijlstra GM, Michielsen MJF, de Gooijer CD, Tramper J. Selection criteria for effective ligands in extractive fermentations with animal cells. of special interest Bioseparation. 1998; This study characterizes the effects of ligands coupled to PEG on both product and cell partitioning.
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