Hepatitis C virus NS5B protein is a membrane-associated phosphoprotein with a predominantly perinuclear localization

Virology

Volumn 227, Issue 2, 1997, Pages 439-446

  Hwang, Soon B ^a   Park, Kyu Jin ^a   Kim, Yong Sun ^a   Sung, Young Chul ^b   Lai, Michael M C ^c  

^a Hallym University   (South Korea)

^b Pohang University of Science and Technology (POSTECH)   (South Korea)

^c UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; PHOSPHOPROTEIN; RNA POLYMERASE; VIRUS PROTEIN;

ARTICLE; CONTROLLED STUDY; HEPATITIS C VIRUS; IMMUNOFLUORESCENCE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LOCALIZATION;

HEPATITIS C VIRUS; INSECTA; MAMMALIA;

EID: 0031579246     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.1996.8357     Document Type: Article

References (36)

1. Behrens, S. E., Tomei, L., and De Francesco, R. (1996). Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus. EMBO J. 15, 12-22.
2. Butterworth, B. E., Shimshick, E. J., and Yin, F. H. (1976). Association of the polioviral RNA complex with phospholipid membrane. J. Virol. 19, 457-466.
3. Cho, Y.-G., Yoon, J.-W., Jang, K.-L., Kim, C.-M., and Sung, Y.-C. (1993). Full genome cloning and nucleotide sequence analysis of hepatitis C virus from sera of chronic hepatitis patients in Korea. Mol. Cells 3, 195-202.
4. Choo, Q.-L., Kuo, G., Weiner, A. J., Overby, L. R., Bradley, D. W., and Houghton, M. (1989). Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244, 359-362.
5. Dennis, D. E., and Brian, D. A. (1982). RNA-dependent RNA polymerase activity in coronavirus-infected cells. J. Virol. 42, 153-164.
6. Enami, M., and Enami, K. (1996). Influenza virus hemagglutinin and neuraminidase glycoproteins stimulate the membrane association of the matrix protein. J. Virol. 70, 6653-6657.
7. Failla, C., Tomei, L., and De Francesco, R. (1994). Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins. J. Virol. 68, 3753-3760.
8. Fuerst, T. A., Niles, E. G., Studier, F. W., and Moss, B. (1986). Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesize bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83, 8122-8126.
9. Gluzman, Y. (1981). SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23, 175-182.
10. Grakoui, A., McCourt, D. W., Wychowski, C., Feinstone, S. M., and Rice, C. M. (1993a). Characterization of the hepatitis C virus-encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites. J. Virol. 67, 2832-2843.
11. Grakoui, A., McCourt, D. W., Wychowski, C., Feinstone, S. M., and Rice, C. M. (1993b). A second hepatitis C virus-encoded proteinase. Proc. Natl. Acad. Sci. USA 90, 10583-10587.
12. Grakoui, A., Wychowski, C., Lin, C., Feinstone, S. M., and Rice, C. M. (1993c). Expression and identification of hepatitis C virus polyprotein cleavage products. J. Virol. 67, 1385-1395.
13. Hahm, B., Han, D. S., Back, S. H., Song, O.-K., Cho, M.-J., Kim, C.-J., Shimotohno, K., and Jang, S. K. (1995). NS3-4A of hepatitis C virus is a chymotrypsin-like protease. J. Virol. 69, 2534-2539.
14. Han, J. H., Shyamala, V., Richman, K. H., Brauer, M. J., Irvine, B., Urdea, M. S., Tekamp-Olson, P., Kuo, G., Choo, Q.-L., and Houghton, M. (1991). Characterization of the terminal regions of hepatitis C viral RNA: Identification of conserved sequences in the 5′ untranslated region and poly(A) tails at the 3′ end. Proc. Natl. Acad. Sci. USA 88, 1711-1715.
15. Hijikata, M., Kato, N., Ootsuyama, Y., Nakagawa, M., and Shimotohno, K. (1991). Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis. Proc. Natl. Acad. Sci. USA 88, 5547-5551.
16. Hijikata, M., Mizushima, H., Akagi, T., Mori, S., Kakiuchi, N., Kato, N., Tanaka, T., Kimura, K., and Shimotohno, K. (1993). Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus. J. Virol. 67, 4665-4675.
17. Hwang, S. B., and Lai, M. M. C. (1993). A unique conformation at the carboxyl terminus of the small hepatitis delta antigen revealed by a specific monoclonal antibody. Virology 193, 924-931.
18. Hwang, S. B., and Lai, M. M. C. (1994). Isoprenylation masks a conformational epitope and enhances trans-dominant inhibitory function of the large hepatitis delta antigen. J. Virol. 68, 2958-2964.
19. Hwang, S. B., Lee, C. Z., and Lai, M. M. C. (1992). Hepatitis delta antigen expressed by recombinant baculoviruses: Comparison of biochemical properties and post-translational modifications between the large and small forms. Virology 190, 413-422.
20. Inchauspe, G., Zebedee, S., Lee, D.-H., Sugitani, M., Nasoff, M., and Prince, A. M. (1991). Genomic structure of the human prototype strain H of hepatitis C virus: Comparison with American and Japanese isolates. Proc. Natl. Acad. Sci. USA 88, 10292-10296.
21. Kato, N., Hijikata, M., Ootsuyama, Y., Nakagawa, M., Ohkoshi, S., Sugimura, T., and Shimotohno, K. (1990). Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proc. Natl. Acad. Sci. USA 87, 9524-9528.
22. Kuo, G., Choo, Q.-L., Alter, H. J., Gitnick, G. L., Redeker, A. G., Purcell, R. H., Miyamura, T., Dienstag, J. L., Alter, M. J., Stevens, C. E., Tegtmeier, G. E., Bonini, F., Colombo, M., Lee, W. S., Kuo, C., Berger, K., Shuster, J. R., Overby, L. R., Brafley, D. W., and Houghton, M. (1989). An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science 244, 362-364.
23. Lazarus, L. H., and Barzilai, R. (1974). Association of foot-and-mouth disease virus replicase with RNA template and cytoplasmic membranes. J. Gen. Virol. 23, 213-218.
24. Lin, C., Lindenbach, B. D., Pragai, B. M., McCourt, D. W., and Rice, C. M. (1994). Processing in the hepatitis C virus E2-NS2 region: Identification of p7 and two distinct E2-specific products with different C termini. J. Virol. 68, 5063-5073.
25. Lin, C., and Rice, C. M. (1995). The hepatitis C virus NS3 serine proteinase and NS4A cofactor: Establishment of a cell-free trans-processing assay. Proc. Natl. Acad. Sci. USA 92, 7622-7626.
26. Manabe, S., Fuke, I., Tanishita, Q., Kaji, C., Gomi, Y., Yoshida, S., Mori, C., Takamizawa, A., Yoshida, I., and Okayama, H. (1994). Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198, 636-644.
27. Matsumoto, M., Hwang, S. B., Jeng, K.-S., Zhu, N., and Lai, M. M. C. (1996). Homotypic interaction and multimerization of hepatitis C virus core protein. Virology 218, 43-51.
28. Matsuura, Y., Suzuki, T., Suzuki, R., Sato, M., Aizaki, H., Saito, I., and Miyamura, T. (1994). Processing of E1 and E2 glycoproteins of hepatitis C virus expressed in mammalian and insect cells. Virology 205, 141-150.
29. Miller, L. K. (1988). Baculoviruses as gene expression vectors. Annu. Rev. Microbiol. 42, 177-199.
30. Miller, R. H., and Purcell, R. H. (1990). Hepatitis C virus shares amino acids sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups. Proc. Natl. Acad. Sci. USA 87, 2057-2061.
31. Saito, I., Miyamura, T., Ohbayashi, A., Harada, H., Katayama, T., Kikuchi, S., Watanabe, Y., Koi, S., Onji, M., Ohta, Y., Choo, Q.-L., Houghton, M., and Kuo, G. (1990). Hepatitis C virus infection is associated with the development of hepatocellular carcinoma. Proc. Natl. Acad. Sci. USA 87, 6547-6549.
32. Sanderson, C. M., Wu, H.-H., and Nayak, D. P. (1994). Sendai virus M protein binds independently to either the F or the HN glycoprotein in vivo. J. Virol. 68, 69-76.
33. Sanger, F., Nicklen, S., and Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
34. Shimotohno, K. (1993). Hepatocellular carcinoma in Japan and its linkage to infection with hepatitis C virus. Semin. Virol. 4, 305-312.
35. Takamizawa, A., Mori, C., Fuke, I., Manabe, S., Murakami, S., Fujita, J., Onishi, E., Andoh, T., Yoshida, I., and Okayama, H. (1991). Structure and organization of the hepatitis C virus genome isolated from human carriers. J. Virol. 65, 1105-1113.
36. Tomei, L., Failla, C., Santolini, E., De Francesco, R., and La Monica, N. (1993). NS3 is a serine protease required for processing of hepatitis C virus polyprotein. J. Virol. 67, 4017-4026.