Cloning of the malic enzyme gene from Corynebacterium glutamicum and role of the enzyme in lactate metabolism

Applied and Environmental Microbiology

Volumn 66, Issue 7, 2000, Pages 2981-2987

  Gourdon, Pierre ^a   Baucher, Marie France ^b   Lindley, Nic D ^a   Guyonvarch, Armel ^b  

^a INSA   (France)

^b UNIVERSITÉ PARIS SACLAY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

MALATE DEHYDROGENASE (DECARBOXYLATING);

ARTICLE; BACTERIAL GROWTH; CARBOXYLATION; CORYNEBACTERIUM GLUTAMICUM; ENZYME ACTIVITY; MOLECULAR CLONING; NONHUMAN;

CATIONS, DIVALENT; CLONING, MOLECULAR; CORYNEBACTERIUM; HYDROGEN-ION CONCENTRATION; KINETICS; LACTATES; MALATE DEHYDROGENASE; MOLECULAR SEQUENCE DATA; NADP; POTASSIUM; QUATERNARY AMMONIUM COMPOUNDS; SEQUENCE ANALYSIS, DNA; TEMPERATURE;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); CORYNEBACTERIUM GLUTAMICUM; PROKARYOTA; UNCULTURED ACTINOMYCETE;

EID: 0033931565     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.66.7.2981-2987.2000     Document Type: Article

References (52)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1987. Current protocols in molecular biology. John Wiley and Sons, New York, N.Y.
2. Birnboim, H. C. 1983. A rapid alkaline extraction method for the isolation of plasmid DNA. Methods Enzymol. 100:243-255.
3. Blattner, F. R., G. Plunkett, C. A. Bloch, N. T. Perna, V. Burland, M. Riley, J. Collado-Vides, F. D. Glasner, C. K. Rode, G. F. Mayhew, J. Gregor, N. W. Davis, H. A. Kirkpatrick, M. A. Goeden, D. J. Rose, B. Man, and Y. Shao. 1997. The complete genome sequence of Escherichia coli K-12. Science 277:1453-1474.
4. Bonamy, C., A. Guyonvarch, O. Reyes, F. David, and G. Leblon. 1990. Interspecies electro-transformation in Corynebacteria. FEMS Microbiol. Lett. 66:263-270.
5. Brosius, J. 1988. A survey of molecular cloning vectors and their uses, p. 205-225. In R. L. Rodriguez and D. T. Denhardt (ed.), Vectors, Butterworth, Boston, Mass.
6. Caruthers, M. H., A. D. Barone, S. L. Beaucage, D. R. Dodds, E. F. Fischer, L. J. McBride, M. Matteuci, Z. Staninsky, and J. Y. Tang. 1985. Chemical synthesis of deoxynucleotides by the phosphoramidite method. Science 230: 281.
7. Chen, F., Y. Okabe, K. Osano, and S. Tajima. 1997. Purification and characterization of the NADP-malic enzyme from Bradyrhizobium japonicum A1017. Biosci. Biotectmol. Biochem. 61:384-386.
8. Cocaign-Bousquet, M., C. Monnet, and N. D. Lindley. 1993. Batch kinetics of Corynebacterium glutamicum during growth on various substrates: use of substrate mixtures to localise metabolic bottlenecks. Appl. Microbiol. Biotechnol. 40:526-530.
9. Cocaign-Bousquet, M., and N. D. Lindley. 1995. Pyruvate overflow and carbon flux within the central metabolic pathways of Corynebacterium glutamicum during growth on lactate. Enzyme Microb. Technol. 17:260-267.
10. Cocaign-Bousquet, M., A. Guyonvarch, and N. D. Lindley. 1996. Growth rate-dependent modulation of carbon flux through central metabolism and the kinetic consequences for glucose-limited chemostat cultures of Corynebacterium glutamicum. Appl. Environ. Microbiol. 62:429-436.
11. Corpet, F., J. Gouzy, and D. Kahn. 1998. The ProDom database of protein domain families. Nucleic Acids Res. 26:323-326.
12. Delaunay, S., D. Uy, M. F. Baucher, J. M. Engasser, A. Guyonvarch, and J. L. Goergen. 1999. Importance of phosphoenolpyruvate carboxylase of Corynebacterium glutamicum during the temperature triggered glutamic acid fermentation. Metab. Eng. 1:334-343.
13. Dominguez, H., C. Rollin, A. Guyonvarch, J.-L. Guerquin-Kern, and N. D. Lindley. 1998 Carbon flux distribution in the central metabolic pathways of Corynebacterium glutamicum during growth on fructose. Eur. J. Biochem. 254:96-102.
14. Dower, W. J., J. F. Miller, and C. W. Ragsdale. 1988. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 1:6127-6145.
15. +-dependent malic enzymes of Rhizobium (Sinorhizobium) meliloti and differential expression of their genes in nitrogen-fixing bacteroids. Microbiology 143:489-498.
16. Fleishmann, R. D., M. D. Adams, O. White, R. A. Clayton, E. F. Kirkness, A. R. Kerlavage, C. J. Bult, J. F. Tomb, B. A. Dougherty, J. M. Merrick, K. McKenney, G. Sutton, W. Fitzhugh, C. A. Fields, J. D. Gocayne, J. D. Scott, R. Shirley, L. I. Liu, A. Glodek, J. M. Kelley, J. F. Weidman, C. A. Phillips, T. Spriggs, E. Hedblom, M. D. Cotton, T. R. Utterback, M. C. Hanna, D. T. Nguyen, D. M. Saudek, R. C. Brandon, L. D. Fine, J. L. Fritschman, J. L. Fuhrmann, N. S. M. Geoghagen, C. L. Gnehm, L. A. McDonald, K. V. Small, C. M. Fraser, H. O. Smith, and J. C. Venter. 1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
17. Garrido-Pertierra, A., C. Martinet Marcos, M. Martin Fernandez, and M. Ruiz-Amil. 1983. Properties and function of malate enzyme from Pseudomanas putida. Biochimie 65:629-635.
18. Gourdon, P., and N. D. Lindley. 1999. Metabolic analysis of glutamate production by Corynebacterium glutamicum. Metab. Eng. 1:224-231.
19. Gubler, M. E., M. S. M. Jetten, S. H. Lee, and A. J. Sinskey. 1994. Effects of phosphoenol pyruvate carboxylase deficiency on metabolism and lysine production in Corynebacterium glutamicum. Appl. Microbiol. Biotechnol. 40: 857-863.
20. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
21. Jetten, M. S. M., and A. J. Sinskey. 1993. Characterization of phosphoenolpyruvate carboxykinase from Corynebacterium glutamicum. FEMS Microbiol. Lett. 111:183-188.
22. Jetten, M. S. M., and A. J. Sinskey. 1995. Purification and properties of oxaloacetate decarboxylase from Corynebacterium glutamicum. Antonie Leeuwenhoek 67:221-227.
23. Kawai, S., H. Suzuki, K. Yamamoki, M. Inui, H. Yakawa, and H. Kumagai. 1996. Purification and characterization of a malic enzyme from the ruminal bacterium Streptococcus bovis ATCC 15352 and cloning and sequencing of its gene. Appl. Environ. Microbiol. 62:2692-2700.
24. Kobayashi, K., S. Doi, S. Negoro, I. Urabe, and H. Okada. 1989. Structure and properties of malic enzyme from Bacillus stearothermophilus. J. Biol. Chem. 6:3200-3205.
25. Kulkarni, G., P. F. Cook, and B. G. Harris. 1993. Cloning and nucleotide sequence of a full-length cDNA encoding Ascaris suum malic enzyme. Arch. Biochem. Biophys. 300:231-237.
26. Lapidus, A., N. Galleron, A. Sorokin, and S. D. Ehrlich. 1997. Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrbB-dnaB region. Microbiology 143:3431-3441.
27. Mallick, S., B. G. Harris, and P. F. Cook. 1991. Kinetic mechanism of NAD:malic enzyme from Ascaris suum in the direction of reductive carboxylation. J. Biol. Chem. 266:2732-2738.
28. Malumbres, M., J. A. Gil, and J. F. Martin. 1993. Codon preference in Corynebacteria. Gene 134:15-24.
29. Marx, A., K. Striegel, A. A. de Graaf, H. Sahm, and L. Eggeling. 1997. Response of the central metabolism of Corynebacterium glutamicum to different flux burdens. Biotechnol. Bioeng. 56:168-180.
30. Marx, A., B. J. Eikmanns, H. Sahm, A. A. de Graaf, and L. Eggeling. 1999. Response of the central metabolism in Corynebacterium glutamicum to the use of an NADH-dependent glutamate dehydrogenase. Metab. Eng. 1:35-48.
31. Molenaar, D., M. E. van der Reste, and S. Petrovic. 1998. Biochemical and genetic characterization of the membrane associated malate dehydrogenase (acceptor) from Corynebacterium glutamicum. Eur. J. Biochem. 254:395-403.
32. Mori, M., and I. Shiio. 1985. Purification and some properties of phosphoenolpyruvate carboxylase from Brevibacterium flavum and its aspartate-overproducing mutant. J. Biochem. 97:1119-1128.
33. Mori, M., and I. Shiio. 1985. Synergistie inhibition of phosphoenolpyruvate carboxylase by aspartate and 2-oxoglutarate in Brevibacterium flavum. J. Biochem. 98:1621-1630.
34. Nilsson, B., M. Uhlen, S. Josephson, S. Gatenbeck, and L. Philipson. 1983. An improved positive selection vector constructed by oligonucleotide mediated mutagenesis. Nucleic Acids Res. 11:8019-8030.
35. O'Regan, M., G. Thierbath, B. Bachmann, D. Villeval, P. Lepage, J. F. Viret, and Y. Lemoine. 1989. Cloning and nucleotide sequence of phosphoenolpyruvate carboxylase-coding gene of Corynebacterium glutamicum ATCC13032. Gene 77:237-251.
36. 13C-NMR spectroscopy and GC-MS. Appl. Microbiol. Biotechnol. 47:430-440.
37. Peters-Wendisch, P. G., B. J. Eikmanns, G. Thierbach, B. Bachmann, and H. Sahm. 1993. Phosphoenolpyruvate carboxylase in Corynebacterium glutamicum is dispensable for growth and lysine production. FEMS Microbiol. Lett. 112:269-274.
38. Peters-Wendisch, P. G., W. F. Wendisch, S. Paul, B. J. Eikmanns, and H. Sahm. 1997. Pyruvate carboxylase as an anaplerotic enzyme in Corynebacterium glutamicum. Microbiology 143:1095-1103.
39. Peters-Wendisch, P. G., C. Kreutzer, J. Kalinowski, M. Patek, H. Sahm, and B. J. Eikmanns. 1998. Pyruvate carboxylase from Corynebacterium glutamicum: characterisation, expression and inactivation of the pyc gene. Microbiology 144:915-927.
40. Reinscheid, D. J., B. J. Eikmanns, and H. Sahm. 1994. Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of the enzyme. J. Bacteriol. 176:3474-3483.
41. Reinscheid, D. J., B. J. Eikmanns, and H. Sahm. 1994. Malate synthase from Corynebacterium glutamicum: sequence analysis of the gene and biochemical characterization of the enzyme. Microbiology 140:3099-3108.
42. Reyes, O., A. Guyonvarch, C. Bonamy, V. Salti, F. David, and G. Leblon. 1991. Integron-bearing vectors: a method suitable for stable chromosomal integration in highly restrictive Corynebacteria. Gene 107:61-68.
43. Rosenberg, M., and D. Court. 1979. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu. Rev. Genet. 13: 319-353.
44. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
45. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
46. Sano, K., K. Ito, K. Miwa, and S. Nakamori. 1987. Amplification of the phosphoenolpyruvate carboxylase gene of Brevibacterium glutamicum to improve amino acid production. Agric. Biol. Chem. 51:597-599.
47. 13C NMR studies of the fluxes in the central metabolism of Corynebacterium glutamicum during growth and overproduction of amino acids in batch cultures. Appl. Microbiol. Biotechnol. 44:489-495.
48. +-dependent malic enzyme in an Escherichia coli mutant. Appl. Environ. Microbiol. 63:2695-2701.
49. Tinoco, I., P. N. Borer, B. Dengler, M. D. Levine, O. C. Uhlenbeck, D. M. Crothers, and J. Gralla. 1973. Improved estimation of secondary structure in ribonucleic acid. Nat. New Biol. 246:40-41.
50. Tosaka, O., H. Morioka, and K. Takinami. 1979. The role of biotin-dependent pyruvate carboxylase in L-lysine production. Agric. Biol. Chem. 43: 1513-1519.
51. Vallino, J. J., and G. Stephanopoulos. 1993. Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol. Bioeng. 41:633-646.
52. Wierenga, R. K., P. Terpstra, and W. G. J. Hol. 1986. Prediction of the occurrence of the ADP-binding βαβ fold in proteins, using an amino acid sequence fingerprint. J. Mol. Biol. 187:101-107.