Pyruvate carboxylase as an anaplerotic enzyme in Corynebacterium glutamicum

Microbiology

Volumn 143, Issue 4, 1997, Pages 1095-1103

  Peters Wendisch, Petra G ^a   Wendisch, Volker F ^a   Paul, Susanne ^a   Eikmanns, Bernhard J ^a   Sahm, Hermann ^a  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

Anaplerotic reactions; Corynebacterium glutamicum; Phosphoenolpyruvate carboxylase; Pyruvate carboxylase

Indexed keywords

ACETIC ACID; ACETYL COENZYME A; ADENOSINE DIPHOSPHATE; ADENOSINE PHOSPHATE; BACTERIAL ENZYME; BIOTIN; CARBON 13; GLUCOSE; LACTIC ACID; PYRUVATE CARBOXYLASE; PYRUVIC ACID;

ARTICLE; BACTERIAL GROWTH; BACTERIUM MUTANT; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME INHIBITION; IMMUNOBLOTTING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

EID: 0031000531     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-143-4-1095     Document Type: Article

References (46)

1. Cazzulo, J. J., Sundaram, T. K. & Kornberg, H. L. (1970). Properties and regulation of pyruvate carboxylase from Bacillus stearothermophilus. Proc R Soc Lond B 16, 1-19.
2. Chao, Y.-P. & Liao, J. C. (1993). Alteration of growth yield by overexpression of phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxykinase in Escherichia coli. Appl Environ Microbiol 59, 4261-4265.
3. Charles, A. M. & Willer, D. W. (1984). Pyruvate carboxylase from Thiobacillus novellus: properties and possible functions. Can J Microbiol 30, 532-539.
4. Cocaign-Bousquet, M. & Lindley, N. D. (1995). Pyruvate overflow and carbon flux within the central metabolic pathways of Corynebacterium glutamicum during growth on lactate. Enzyme Microb Technol 17, 260-267.
5. Cocaign-Bousquet, M., Guyonvarch, A. & Lindley, N. D. (1996). Growth rate-dependent modulation of carbon flux through central metabolism and the kinetic consequences for glucose-limited chemostat cultures of Corynebacterium glutamicum. Appl Environ Microbiol 62, 429-436.
6. Diesterhaft, M. D. & Freese, E. (1973). Role of pyruvate carboxylase, phosphoenolpyruvate carboxykinase and malic enzyme during growth and sporulation of Bacillus subtilis. J Biol Chem 248, 6062-6070.
7. Eikmanns, B. J., Follettie, M. T., Griot, M. U. & Sinskey, A. J. (1989). The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: molecular cloning, nucleotide sequence, and expression. Mol Gen Genet 218, 330-339.
8. Eikmanns, B. J., Metzger, M., Reinscheid, D., Kircher, M. & Sahm, H. (1991). Amplification of three theonine biosynthesis genes in Corynebacterium glutamicum and its influence on carbon flux in different strains. Appl Microbiol Biotechnol 34, 617-622.
9. Encarnacion, S., Dunn, M., Willms, K. & Mora, J. (1995). Fermentative and aerobic metabolism in Rhizobium etli. J Bacteriol 177, 3058-3066.
10. Feir, H. A. & Suzuki, I. (1969). Pyruvate carboxylase of Aspergillus niger: kinetic study of a biotin-containing carboxylase. Can J Biochem 47, 697-710.
11. Fisher, S. H. & Magasanik, B. (1984). Synthesis of oxaloacetate in Bacillus subtilis mutants lacking the 2-ketoglutarate dehydrogenase enzymatic complex. J Bacteriol 158, 55-62.
12. Gornall, A. G., Bardawill, C. J. & David, M. M. (1949). Determination of serum proteins by means of the biuret reaction. J Biol Chem 177, 751-766.
13. Gubler, M., Park, S. M., Jetten, M., Stephanopoulos, G. & Sinskey, A. J. (1994). Effects of phosphoenolpyruvate carboxylase deficiency on metabolism and lysine production in Corynebacterium glutamicum. Appl Microbiol Biotechnol 40, 857-863.
14. Gurr, J. A. & Jones, K. M. (1977). Purification and characterization of pyruvate carboxylase from Arthrobacter globiformis. Arch Biochem Biophys 179, 444-455.
15. Jäger, W., Peters-Wendisch, P. G., Kalinowski, J. & Pühler, A. (1996). A Corynebacterium glutamicum gene encoding a two-domain protein similar to biotin carboxylases and biotin-carboxyl-carrier proteins. Arch Microbiol 166, 76-82.
16. Jetten, M. S. M. & Sinskey, A. J. (1993). Characterization of phosphoenolpyruvate carboxykinase from Corynebacterium glutamicum. FEMS Microbiol Lett 111, 183-188.
17. Jetten, M. S. M. & Sinskey, A. J. (1995). Purification and properties of oxaloacetate decarboxylase from Corynebacterium glutamicum. Antonie Leeuwenhoek 67, 221-227.
18. Jetten, M. S. M., Pitoc, G. A., Follettie, M. T. & Sinskey, A. J. (1994). Regulation of phospho(enol)-pyruvate-and oxaloacetate-converting enzymes in Corynebacterium glutamicum. Appl Microbiol Biotechnol 41, 47-52.
19. Kawamura, F. & Doi, R. H. (1984). Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases. J Bacteriol 160, 442-444.
20. Kinoshita, S. (1985). Glutamic acid bacteria. In Biology of Industrial Microorganisms, pp. 115-142. Edited by A. Demain & N. Solomon. London: Benjamin/Cummings Publishing.
21. Kornberg, H. L. (1966). Anaplerotic sequences and their role in metabolism. In Essays in Biochemistry, vol. 2, pp. 1-31. Edited by P. N. Campbell & G. P. Greville. New York: Academic Press.
22. Krulwich, T. A. & Pelliccione, N. J. (1979). Catabolic pathways of coryneforms, nocardias and mycobacteria. Ann Rev Microbiol 33, 95-111.
23. Liebl, W. (1991). The genus Corynebacterium - nonmedical. In The Prokaryotes, vol. II, pp. 1157-1171. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer-Verlag.
24. Liebl, W. & Schein, B. (1990). Isolation of restriction deficient mutants of Corynebacterium glutamicum. In DECHEMA Biotechnology Conferences, vol. 4, pp. 323-327. Edited by D. Behrens & P. Krämer. Weinheim: Verlag Chemie.
25. Milrad de Forchetti, S. R. & Cazzulo, J. J. (1976). Some properties of the pyruvate carboxylase from Pseudomonas fluorescens. J Gen Microbiol 93, 75-81.
26. Modak, H. V. & Kelly, D. J. (1995). Acetyl-CoA-dependent pyruvate carboxylase from the photosynthetic bacterium Rhodobacter capsulatus: rapid and efficient purification using dye-ligand affinity chromatography. Microbiology 141, 2619-2628.
27. Mori, M. & Shiio, I. (1985a). Purification and some properties of phosphoenolpyruvate carboxylase from Brevibacterium flavum and its aspartate-overproducing mutant. J Biochem 97, 1119-1128.
28. Mori, M. & Shiio, I. (1985b). Synergistic inhibition of phosphoenolpyruvate carboxylase by aspartate and 2-oxoglutarate in Brevibacterium flavum. J Biochem 98, 1621-1630.
29. O'Brien, R. W., Chuang, D. T., Taylor, B. L. & Utter, M. F. (1977). Novel enzymic machinery for the metabolism of oxaloacetate, phosphoenolpyruvate and pyruvate in Pseudomonas citronellolis. J Biol Chem 252, 1257-1263.
30. Ozaki, H. & Shiio, I. (1969). Regulation of the TCA and glyoxylate cycles in Brevibacterium flavum. J Biochem 66, 297-311.
31. Payne, J. & Morris, J. G. (1969). Pyruvate carboxylase in Rhodopseudomonas sphaeroides. J Gen Microbiol 59, 97-101.
32. Peters-Wendisch, P. G., Eikmanns, B. J., Thierbach, G., Bachmann, B. & Sahm, H. (1993). Phosphoenolpyruvate carboxylase in Corynebacterium glutamicum is dispensable for growth and lysine production. FEMS Microbiol Lett 112, 269-274.
33. 3-Carboxylation as an anaplerotic reaction in phosphoenolpyruvate carboxylase-deficient Corynebacterium glutamicum. Arch Microbiol 165, 387-396.
34. Ruiz-Amil, M., deTorrontegui, G., Palacian, E., Catalina, L. & Losada, M. (1965). Properties and functions of yeast pyruvate carboxylase. J Biol Chem 240, 3485-3492.
35. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
36. Schobert, P. & Bowien, B. (1984). Unusual C3 and C4 metabolism in the chemoautotroph Alcaligenes eutrophus. J Bacteriol 159, 167-172.
37. Schrumpf, B., Eggeling, L. & Sahm, H. (1992). Isolation and prominent characteristics of an L-lysine hyperproducing strain of Corynebacterium glutamicum. Appl Microbiol Biotechnol 37, 566-571.
38. Scrutton, M. C. & Taylor, B. L. (1974). Isolation and characterization of pyruvate carboxylase from Azotobacter vinelandii OP. Arch Biochem Biophys 164, 641-654.
39. Scrutton, M. C. & Young, M. R. (1972). Pyruvate carboxylase. In The Enzymes, vol. 6, pp. 1-35. Edited by P. D. Boyer. New York: Academic Press.
40. Seubert, W. & Weicker, H. (1969). Pyruvate carboxylase from Pseudomonas. Methods Enzymol 13, 258-262.
41. Sundaram, T. K. (1973). Physiological role of pyruvate carboxy-lase in a thermophilic bacillus. J Bacteriol 113, 549-557.
42. Tosaka, O., Morioka, H. & Takinami, K. (1979). The role of biotin-dependent pyruvate carboxylase in L-lysine production. Agric Biol Chem 43, 1513-1519.
43. Vallino, J. J. & Stephanopoulos, G. (1993). Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol Bioeng 41, 633-646.
44. Willison, J. C. (1988). Pyruvate and acetate metabolism in the photosynthetic bacterium Rhodobacter capsulatus. J Gen Microbiol 134, 2429-2439.
45. 2 fixation in metabolism. In Essays in Biochemistry, vol. 2, pp. 1-27. Edited by P. N. Campbell & G. D. Greville. New York: Academic Press.
46. Young, M. R., Tolbert, B. & Utter, M. F. (1969). Pyruvate carboxylase from Saccharomyces cerevisiae. Methods Enzymol 13, 250-258.