A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis

Chemistry and Biology

Volumn 7, Issue 11, 2000, Pages 895-905

  Aparicio, Jesús F ^a,b   Fouces, Roberto ^a   Mendes, Marta V ^a   Olivera, Noemí ^a   Martín, Juan F ^a,b  

^a Institute of Biotechnology INBIOTEC Parque Científico de León   (Spain)

^b UNIVERSITY OF LEÓN   (Spain)

Author keywords

Macrolide; Pimaricin; Polyene; Polyketide synthase; Streptomyces natalensis

Indexed keywords

ANTIFUNGAL ACTIVITY; CHEMICAL STRUCTURE; GENE CLUSTER; GENE DISRUPTION; MACROLIDE; MICROBIAL CONTAMINATION; MULTIENZYME COMPLEX; NATAMYCIN; OPEN READING FRAME; POLYENE; POLYKETIDE SYNTHASE;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; ANTIBIOTICS, ANTIFUNGAL; CHROMOSOME WALKING; CODON; GENE LIBRARY; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; MULTIENZYME COMPLEXES; MULTIGENE FAMILY; NATAMYCIN; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; STREPTOMYCES;

EID: 0033763409     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(00)00038-7     Document Type: Article

References (63)

1. Polyene antibiotics
2. Molecular genetics of polyketides and its comparison to fatty acid biosynthesis
3. The extraordinary enzymes involved in erythromycin biosynthesis
4. Genetic contributions to understanding polyketide synthases
5. The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin
6. Repeated polyketide synthase modules involved in the biosynthesis of a heptaene macrolide by Streptomyces sp. FR-008
7. The biosynthetic gene cluster for the 26-membered ring polyene macrolide pimaricin
8. Modular organisation of genes required for complex polyketide biosynthesis
9. An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea
10. Limited proteolysis and active-site studies of the first multienzyme component of the erythromycin-producing polyketide synthase
11. Stereospecific acyl transfers on the erythromycin-producing polyketide synthase
12. Divergent sequence motifs correlated with the substrate specificity of (methyl)malonyl-CoA: Acyl carrier protein transacylase domains in modular polyketide synthases
13. Biosynthesis of fatty acid and polyketide metabolites
14. Biosynthesis of polyene macrolide antibiotics
15. How do the polyene macrolide antibiotics affect the cellular membrane properties?
16. Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: Analysis of the gene cluster and deduction of the biosynthetic pathway
17. Codon usage in the G+C-rich Streptomyces genome
18. An acyl-carrier-protein-thioesterase domain from the 6-deoxyerythronolide B synthase of Saccharopolyspora erythraea
19. Organisation of the biosynthetic gene cluster for the polyketide antihelmintic macrolide avermectin in Streptomyces avermitilis
20. The biosynthetic gene cluster for the microtubule-stabilising agents epothilones A and B from Sorangium celluiosum So ce90
21. Cloning and heterologous expression of the epothilone gene cluster
22. Repositioning of a domain in a modular polyketide synthase to promote specific chain cleavage
23. Biosynthesis of the ansamycin antibiotic rifamycin: Deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699
24. Engineering of a minimal modular polyketide synthase, and targeted alteration of the stereospecificity of polyketide chain extension
25. Evidence for a double-helical structure for modular polyketide synthases
26. 6-Deoxyerythronolide-B synthase 2 from Saccharopolyspora erythraea
27. Organisation of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora erythraea
28. Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: Analysis of the enzymatic domains in the modular polyketide synthase
29. Characterisation of a Streptomyces antibioticus gene encoding a type I polyketide synthase which has an unusual coding sequence
30. Crystal structure of β-ketoacyl-acyl carrier protein synthase II from E. coli reveals the molecular architecture of condensing enzyme
31. Identification and characterisation of the niddamycin polyketide synthase genes from Streptomyces caelestis
32. A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: Architecture of metabolic diversity
33. A chain initiation factor common to both modular and aromatic polyketide synthases
34. Conversion of a β-ketoacyl synthase to a malonyl decarboxylase by replacement of the active-site cysteine with a glutamine
35. Redesign of the coenzyme specificity of a dehydrogenase by protein engineering
36. Prediction of the occurrence of the ADP-binding βαβ-fold in proteins, using an amino acid sequence fingerprint
37. Cloning and sequencing analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: Sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases
38. An erythromycin derivative produced by targeted gene disruption in Saccharopolyspora erythraea
39. Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis
40. A cytochrome P450-like gene possibly involved in oleandomycin biosynthesis by Streptomyces antibioticus
41. Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: Analysis of genes flanking the polyketide synthase
42. High-resolution crystal-structure of cytochrome P450(CAM)
43. Structure of cytochrome P450(ERYF) involved in erythromycin biosynthesis
44. Occurrence and biological function of cytochrome P450 monooxygenases in actinomycetes
45. Cloning and heterologous expression of the genes encoding nonspecific electron transport components for a cytochrome P450 system of Saccharopolyspora erythraea involved in erythromycin production
46. Sequence of gene choB encoding cholesterol oxidase of Brevibacterium sterolicum: Comparison with choA of Streptomyces sp. SA-COO
47. Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts
48. Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp.
49. The pab gene from Streptomyces griseus, encoding p-aminobenzoic acid synthase, is located between genes possibly involved in candicidin biosynthesis
50. Impact of thioesterase activity on tylosin biosynthesis in Streptomyces fradiae
51. Nucleotide sequence and cloning in Bacillus subtilis of the Bacillus stearothermophilus pleiotropic regulatory gene degT
52. Molecular characterisation of a gene from Saccharopolyspora erythraea which is involved in erythromycin biosynthesis
53. 1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius
54. TTA codons in some genes prevent their expression in a class of developmental, antibiotic-negative, Streptomyces mutants
55. Direct evidence that the rifamycin polyketide synthase assembles polyketide chains processively
56. The biosynthetic gene cluster for the macrolactone ring of the immunosuppressant FK506
57. Bacterial cholesterol oxidases are able to act as flavoprotein-linked ketosteroid monooxygenases that catalyse the hydroxylation of cholesterol to 4-cholesten-6-ol-3-one
58. New derivatives of the Streptomyces temperate phage OC31 useful for the cloning and functional analysis of Streptomyces DNA
59. Restriction mapping of the DNA of the Streptomyces temperate phage OC31 and its derivatives
60. DNA sequencing with chain terminating inhibitors
61. A comprehensive set of sequence analysis programs for the VAX