Biodistribution and transgene expression with nonviral cationic vector/DNA complexes in the lungs

Gene Therapy

Volumn 7, Issue 20, 2000, Pages 1753-1760

  Bragonzi, A ^a   Dina, G ^a   Villa, A ^b   Calori, G ^c   Biffi, A ^a   Bordignon, C ^a   Assael, B M ^c   Conese, M ^a  

^a SAN RAFFAELE HOSPITAL   (Italy)

^b UNIVERSITY OF MILANO BICOCCA   (Italy)

^c UNIVERSITY OF MILAN   (Italy)

Author keywords

Barries; Cationic polymers; Gene transfer; Intratracheal; Intravenous; Lipids

Indexed keywords

CATION; DNA; EXGEN 500; GREEN FLUORESCENT PROTEIN; LIPOSOME; LUCIFERASE; MACROGOL; POLYETHYLENEIMINE; SURFACTANT PROTEIN A;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRONCHUS; CELL MEMBRANE; CELL POPULATION; COMPLEX FORMATION; CONTROLLED STUDY; ENDOTHELIUM CELL; ENZYME ACTIVITY; EXPRESSION VECTOR; GENE EXPRESSION; GENE TARGETING; GENE TRANSFER; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LUNG; LUNG ALVEOLUS; MOUSE; MOUSE STRAIN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; TISSUE DISTRIBUTION; TRANSGENE;

ANIMALIA; BRONCHUS;

EID: 0033758920     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301282     Document Type: Article

References (36)

1. Cystic fibrosis clinical trials
2. Status of gene therapy for cystic fibrosis lung disease
3. Cationic lipid-mediated CFTR gene transfer to the lungs and nose of patients with cystic fibrosis: A double-blind placebo-controlled trial
4. A phase I study of adenovirus-mediated transfer of the human cystic fibrosis transmembrane conductance regulator gene to a lung segment of individuals with cystic fibrosis
5. Gene transfer by guanidium-cholesterol cationic lipids into airway epithelial cells in vitro and in vivo
6. ExGen 500 is an efficient vector for gene delivery to lung epithelial cells in vitro and in vivo
7. Comparison between intratracheal and intravenous administration of liposome-DNA complexes for cystic fibrosis lung gene therapy
8. Optimization of formulations and conditions for the aerosol delivery of functional cationic lipid:DNA complexes
9. A concentrated and stable aerosol formulation of cationic lipid:DNA complexes giving high-level gene expression in mouse lung
10. Improved DNA:Liposome complexes for increased systemic delivery and gene expression
11. Factors influencing the efficiency of cationic liposome-mediated intravenous gene delivery
12. Polyethylenimine-based intravenous delivery of transgenes to mouse lung
13. Comparison between cationic polymers and lipids in mediating systemic gene delivery to the lungs
14. Characterization of cationic liposome-mediated gene transfer in vivo by intravenous administration
15. Factors controlling the efficiency of cationic lipid-mediated transfection in vivo via intravenous administration
16. In vivo gene transfer via intravenous administration of cationic lipid-protamine-DNA (LPD) complexes
17. Lung-specific direct in vivo gene transfer with recombinant plasmid DNA
18. Surfactant inhibits cationic liposome-mediated gene transfer
19. Interaction of liposomal and polycationic transfection complexes with pulmonary surfactant
20. A mutation in the surfactant protein B gene responsible for fatal neonatal respiratory disease in multiple kindreds
21. Biodistribution and gene expression of lipid/plasmid complexes after systemic administration
22. Overcoming the inhibitory effect of serum on lipofection by increasing the charge ratio of cationic liposome to DNA
23. Dynamic changes in the characteristics of cationic lipidic vectors after exposure to mouse serum: Implications for intravenous lipofection
24. Nanoscopic structure of DNA condensed for gene delivery
25. Glomerular filtration is required for transfection of proximal tubular cells in the rat kidney following injection of DNA complexes into the renal artery
26. Rapid crossing of the pulmonary endothelial barrier by polyethylenimine/DNA complexes
27. Size, diffusibility and transfection performance of linear PEI/DNA complexes in the mouse central nervous system
28. Safety of a single aerosol administration of escalating doses of the cationic lipid GL-67/DOPE/DMPE-PEG5000 formulation to the lungs of normal volunteers
29. Surfactant enhances adenovirus-mediated gene expression in rabbit lungs
30. In vitro and in vivo transfer and expression of human surfactant SP-A- and SP-B-associated protein cDNAs mediated by replication-deficient, recombinant adenoviral vectors
31. Cationic liposomes target angiogenic endothelial cells in tumors and chronic inflammation in mice
32. Efficiency of gene transfer for restoration of normal airway epithelial function in cystic fibrosis
33. Intratracheal gene delivery to the mouse airway: Characterization of plasmid DNA expression and pharmacokinetics
34. Comparison of DNA-lipid complexes and DNA alone for gene transfer to cystic fibrosis airway epithelia in vivo