Assay for IκB kinases using an in vivo biotinylated IκB protein substrate

Analytical Biochemistry

Volumn 274, Issue 2, 1999, Pages 220-228

  Wisniewski, D ^a   Lograsso, P ^a   Calaycay, J ^a   Marcy, A ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; COENZYMES; PHOSPHORYLATION; TRANSCRIPTION;

GLUTATHIONE S-TRANSFERASES; KINASE ACTIVITY; MYELIN BASIC PROTEIN; PEPTIDE SUBSTRATES; PHOSPHORYLATION ACTIVITIES; PROTEIN SUBSTRATE; REGULATORY SUBUNITS; STAUROSPORINES;

PEPTIDES;

GLUTATHIONE TRANSFERASE; MYELIN BASIC PROTEIN; PROTEIN KINASE; RECOMBINANT ENZYME; STAUROSPORINE; STREPTAVIDIN; BIOTIN; CHUK PROTEIN, HUMAN; I KAPPA B; I KAPPA B KINASE; IKBKB PROTEIN, HUMAN; IKBKE PROTEIN, HUMAN; PROTEIN SERINE THREONINE KINASE;

ARTICLE; BIOTINYLATION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PHOSPHORYLATION; ENZYME PURIFICATION; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; CELL LINE; HUMAN; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS;

BACTERIA (MICROORGANISMS);

BIOTIN; BIOTINYLATION; CELL LINE; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; I-KAPPA B KINASE; I-KAPPA B PROTEINS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PROTEIN-SERINE-THREONINE KINASES;

EID: 0033568924     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4287     Document Type: Article

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