Continuous assay for VanX, the D-alanyl-D-alanine dipeptidase required for high-level vancomycin resistance

Analytical Biochemistry

Volumn 272, Issue 1, 1999, Pages 94-99

  Brandt, Jeffrey J ^a   Chatwood, Lisa L ^a   Yang, Ke Wu ^a   Crowder, Michael W ^a  

^a MIAMI UNIVERSITY   (United States)

Author keywords

Continuous assay; L alanine p nitroanilide; UV VIS spectrophotometry; VanX, Zn(II)

Indexed keywords

AMINO ACIDS; COBALT COMPOUNDS; IRON COMPOUNDS; KINETICS; LACTIC ACID; NICKEL COMPOUNDS; SPECTROPHOTOMETRY;

CONTINUOUS ASSAY; HIGH THROUGHPUT SCREENING; KINETIC MECHANISM; P-NITROANILIDE; P-NITROANILINE; SATURATION KINETICS; UV-VIS SPECTROPHOTOMETRY; VANX, ZN(II);

ZINC COMPOUNDS;

4 NITROANILINE; ALANINE; COBALT; COBALT CHLORIDE; DIPEPTIDASE; FERROUS CHLORIDE; FERROUS ION; LACTIC ACID DERIVATIVE; NICKEL; NICKEL CHLORIDE; VANCOMYCIN; ZINC CHLORIDE; ZINC ION;

ANTIBIOTIC RESISTANCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME PURIFICATION; GENE OVEREXPRESSION; HYDROLYSIS; PRIORITY JOURNAL; SPECTROPHOTOMETRY;

FELIS CATUS;

EID: 0033566230     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4166     Document Type: Article

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