Complete kinetic mechanism of elongation factor Tu-dependent binding of aminoacyl-tRNA to the A site of the E.coli ribosome

EMBO Journal

Volumn 17, Issue 24, 1998, Pages 7490-7497

  Pape, Tillmann ^a   Wintermeyer, Wolfgang ^a   Rodnina, Marina V ^a  

^a UNIVERSITY OF WITTEN HERDECKE   (Germany)

Author keywords

Codon recognition; Conformational change; EF Tu function; GTP hydrolysis; Protein synthesis

Indexed keywords

AMINOACYL TRANSFER RNA; ELONGATION FACTOR TU; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; MAGNESIUM ION; PHENYLALANINE TRANSFER RNA;

ARTICLE; BINDING KINETICS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN RNA BINDING; RIBOSOME;

CODON; COMPUTER SIMULATION; ESCHERICHIA COLI; GUANOSINE TRIPHOSPHATE; HYDROLYSIS; KINETICS; MODELS, CHEMICAL; NUCLEIC ACID CONFORMATION; PEPTIDE CHAIN ELONGATION, TRANSLATIONAL; PEPTIDE ELONGATION FACTOR TU; POLY U; PROTEIN CONFORMATION; RIBOSOMES; RNA, TRANSFER, AMINO ACYL;

ESCHERICHIA COLI;

EID: 0032535207     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.24.7490     Document Type: Article

References (14)

1. Abel, K., Yoder, M.D., Hilgenfeld, R. and Jumak, F. (1996) An α to β conformational switch in EF-Tu. Structure, 4, 1153-1159.
2. Bilgin, N., Claesens, F., Pahverk, H. and Ehrenberg, M. (1992) Kinetic properties of Escherichia coli ribosomes with altered forms of S12. J. Mol Biol., 224, 1011-1027.
3. Dell, V.A., Miller, D.L. and Johnson, A.E. (1990) Effects of nucleotide-and aurodox-induced changes in elongation factor Tu conformation upon its interactions with aminoacyl transfer RNA. A fluorescence study. Biochemistry, 29, 1757-1763.
4. Eccleston, J.K., Dix, D. and Thompson, R.C. (1985) The rate of cleavage of GTP on the binding of Phe-tRNA-elongation factor Tu-GTP to poly(U)-programmed ribosomes of Escherichia coli. J. Biol. Chem., 260, 16237-16241.
5. Fourmy, D., Yoshizawa, S. and Puglisi, J.D. (1998) Paromomycin binding induces a local conformational change in the A-site of 16S rRNA. J. Mol. Biol, 277, 333-345.
6. Grosjean, H., Söll, D.G. and Crothers, D.M. (1976) Studies of the complex between transfer RNAs with complementary codons. I. Origins of enhanced affinity between complementary triplets. J. Mol. Biol., 103, 499-519.
7. Grosjean, H.J., de Henau, S. and Crothers, D.M. (1978) On the physical basis for ambiguity in genetic coding interactions. Proc. Natl Acad. Sci. USA, 75, 610-614.
8. Harmark, K., Anborgh, P.H., Merola, M., Clark, B.F. and Parmeggiani, A. (1992) Substitution of aspartic acid-80, a residue involved in coordination of magnesium, weakens the GTP binding and strongly enhances the GTPase of the G domain of elongation factor Tu. Biochemistry, 31, 7367-7372.
9. Ivell, R., Sander, G. and Parmeggiani, A. (1981) Modulation by monovalent and divalent cations of the guanosine-5′-triphosphate activity dependent on elongation factor Tu. Biochemistry, 20, 6852-6859.
10. Jelenc, P.C. and Kurland, C.G. (1979) Nucleoside triphosphate regeneration decreases the frequency of translation errors. Proc. Natl Acad. Sci. USA, 76, 3174-3178.
11. H-ras. J. Biol. Chem., 268, 923-929.
12. Karim, A.M. and Thompson, R.C. (1986) Guanosine 5′-O-(3-thiotri-phosphate) as an analog of GTP in protein biosynthesis. J. Biol. Chem., 261, 3238-3243.
13. Kaziro,Y. (1978) The role of guanosine-5′-triphosphate in polypeptide chain elongation. Biochim. Biophys. Acta, 505, 95-127.
14. Moazed, D. and Noller, H. (1990) Binding of tRNA to the ribosomal A and P sites protects two distinct sets of nucleotides in 16S rRNA. J. Mol Biol., 211, 135-145.