Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses

EMBO Journal

Volumn 17, Issue 24, 1998, Pages 7239-7249

  Martinho, Rui G ^a   Lindsay, Howard D ^a   Flaggs, Gail ^b   DeMaggio, Anthony J ^b   Hoekstra, Merl F ^b   Carr, Antony M ^a   Bentley, Nicola J ^a  

^a UNIVERSITY OF SUSSEX   (United Kingdom)

^b ELI LILLY AND COMPANY   (United States)

Author keywords

ATM; ATR; Cell cycle checkpoints; Chk1; Rad3

Indexed keywords

PROTEIN KINASE;

ARTICLE; CELL CYCLE G2 PHASE; CELL CYCLE S PHASE; DNA DAMAGE; DNA REPLICATION; DNA STRUCTURE; EUKARYOTIC CELL; NONHUMAN; PRIORITY JOURNAL; SCHIZOSACCHAROMYCES POMBE;

ADENOSINE TRIPHOSPHATASES; AMINO ACID SEQUENCE; DNA DAMAGE; DNA HELICASES; DNA REPLICATION; G2 PHASE; GENE DOSAGE; HYDROXYUREA; INTERPHASE; MOLECULAR SEQUENCE DATA; MUTATION; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN KINASES; PROTEIN-SERINE-THREONINE KINASES; RADIATION TOLERANCE; S PHASE; SELECTION (GENETICS); SUPPRESSION, GENETIC; ULTRAVIOLET RAYS;

EUKARYOTA; SCHIZOSACCHAROMYCES POMBE;

EID: 0032535157     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.24.7239     Document Type: Article

References (30)

1. Al-Khodairy,F., Fotou,E., Sheldrick,K.S., Griffiths,D.J.F., Lehmann,A.R. and Carr,A.M. (1994) Identification and characterisation of new elements involved in checkpoints and feedback controls in fission yeast. Mol. Biol. Cell, 5, 147-160.
2. Barbet,N.C., Muriel,W.J. and Carr,A.M. (1992) Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene, 114, 59-66.
3. Bentley,N.J., Holtzman,D.A., Flaggs,G., Keegan,K.S., DeMaggio,A., Ford,J.C., Hoekstra,M. and Carr,A.M. (1996) The Schizosaccharomyces pombe rad3 checkpoint gene. EMBO J., 15, 6641-6651.
4. Boddy,M.N., Furnari,B., Mondesert,O. and Russell,P. (1998) Replication checkpoint enforced by kinases Cds1 and Chk1. Science, 280, 909-912.
5. Carr,A.M. (1995) DNA structure checkpoints in fission yeast. Semin. Cell Biol., 6, 65-72.
6. Carr,A.M., Moudjou,M., Bentley,N.J. and Hagan,I.M. (1995) The chk1 pathway is required to prevent mitosis following cell-cycle arrest at 'start'. Curr. Biol., 5, 1179-1190.
7. Cliby,W.A., Roberts,C.J., Cimprich,K.A., Stringer,C.M., Lamb,J.R., Schreiber,S.L. and Friend,S.H. (1998) Overexpression of a kinase-inactive ATR protein causes sensitivity to DNA-damaging agents and defects in cell cycle checkpoints. EMBO J., 17, 159-169.
8. Edwards,R.J. and Carr,A.M. (1997) Analysis of radiation-sensitive mutants of fission yeast. Methods Enzymol., 283, 471-494.
9. Flaggs,G. et al. (1997) ATM- and ATR-dependent interactions of a mammalian CHK1 homolog with meiotic chromosomes. Curr. Biol., 7, 977-986.
10. Ford,J.C., Al-Khodairy,F., Fotou,E., Sheldrick,K.S., Griffiths,D. and Carr,A.M. (1994) 14-3-3 protein homologs required for the DNA damage checkpoint in fission yeast. Science, 265, 533-535.
11. Furnari,B., Rhind,N. and Russell,P. (1997) Cdc25 mitotic inducer targeted by Chk1 DNA damage checkpoint kinase. Science, 277, 1495-1497.
12. Griffiths,D.J.F., Barbet,N.C., McCready,S., Lehmann,A.R. and Carr,A.M. (1995) Fission yeast rad17: a homologue of budding yeast RAD24 that shares regions of sequence similarity with DNA polymerase accessory proteins. EMBO J., 14, 5812-5823.
13. Grimm,C., Kohli,J., Murray,J. and Maundrell,K. (1988) Genetic engineering of Schizosaccharomyces pombe: A system for gene disruption and replacement using the ura4 gene as a selectable marker. Mol. Gen. Genet., 215, 81-86.
14. Gutz,H., Heslot,H., Leupold,U. and Loprieno,N. (1974) Schizosaccharomyces pombe. In King,R.C. (ed.), Handbook of Genetics Vol. 1. Plenum Press, New York, NY, pp. 395-446.
15. Hartwell,L.H. and Weinert,T.A. (1989) Checkpoints: controls that ensure the order of cell cycle events. Science, 246, 629-634.
16. Humphreys,G.O., Willshaw,G.A., Smith,H.R. and Anderson,E.S. (1976) Mutagenesis of plasmid DNA with hydroxylamine: isolation of mutants of multi-copy plasmids. Mol. Gen. Genet., 145, 101-108.
17. Kostrub,C., Knudsen,K., Subramani,S. and Enoch,T. (1998) Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage. EMBO J., 17, 2055-2066.
18. Lieberman,H.B., Hopkins,K.M., Nass,M., Demetrick,D. and Davey,S. (1996) A human homologue of the Schizosaccharomyces pombe rad9+ checkpoint control gene. Proc. Natl Acad. Sci. USA, 93, 13890-13895.
19. Lindsay,H.D., Griffiths,D.J.F., Edwards,R., Murray,J.M., Christensen,P.U., Walworth,N. and Carr,A.M. (1998) S phase specific activation of Cds1 kinase defines a subpathway of the checkpoint response in Schizosaccharomyces pombe. Genes Dev., 12, 382-395.
20. Martinho,R.G. and Carr,A.M. (1998) Isolation of DNA structure dependent checkpoint mutants in S.pombe. Humana Press Inc., in press.
21. Maundrell,K. (1990) nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine. J. Biol. Chem., 265, 10857-10864.
22. 2 checkpoint control function. Mol. Gen. Genet., 255, 332-340.
23. Murakami,H. and Okayama,H. (1995) A kinase from fission yeast responsible for blocking mitosis in S phase. Nature, 374, 817-819.
24. Murray,J.M., Doe,C., Schenk,P., Carr,A.M., Lehmann,A.R. and Watts,F.Z. (1992) Cloning and characterisation of the S.pombe rad15 gene, a homologue to the S.cerevisiae RAD3 and human ERCC2 genes. Nucleic Acids Res., 20, 2673-2678.
25. O'Connell,M.J., Raleigh,J.M., Verkade,H.M. and Nurse,P. (1997) Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation. EMBO J., 16, 545-554.
26. 2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216. Science, 277, 1501-1505.
27. Sanches,Y., Wong,C., Thoma,R.S., Richman,R., Wu,Z., Piwnica-Worms,H. and Elledge,S.J. (1997) Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science, 277, 1497-1501.
28. Savitsky,K. et al. (1995) A single ataxia telangiectasia gene with a product similar to PI 3-kinase. Science, 268, 1749-1753.
29. Walworth,N. and Bernards,R. (1996) rad-dependent responses of the chk1-encoded protein kinase at the DNA damage checkpoint. Science, 271, 353-356.
30. Walworth,N., Davey,S. and Beach,D. (1993) Fission yeast chk1 protein kinase links the rad checkpoint pathway to cdc2. Nature, 363, 368-371.