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K. Lundgren et al., ibid. 64, 1111 (1991).
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P. Jin, Y. Gu, D. O. Morgan, J. Cell Biol. 134, 963 (1996); A. Blasina, S. Paegle, C. H. McGowan, Mol. Biol. Cell 8, 1013 (1997).
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Blasina, A.1
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10
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2642624122
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Y. Sanchez et al., ibid., p. 1497; C.-Y. Peng et al., ibid., p. 1501.
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Science
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Sanchez, Y.1
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11
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15444355447
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Y. Sanchez et al., ibid., p. 1497; C.-Y. Peng et al., ibid., p. 1501.
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Science
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Peng, C.-Y.1
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17
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2642599532
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note
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70 truncation product were replicated with a precise fusion of GST and amino acids 1 to 72 of Wee1.
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18
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0026025891
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+. All strains were leu1-32 ura4-D18. All nmt1 promoter constructs were integrated at the leu1-32 locus. Growth media and general methods for S. pombe have been described [S. Moreno, A. Klar, P. Nurse, Methods Enzymol. 194, 795 (1991)]. Unless otherwise indicated, yeast cultures were grown in YES medium at 30°C. YES consists of glucose, yeast extract, and amino acid supplements. HU was used at a concentration of 12 mM. Purification of GST fusion proteins and hexahis-tagged proteins expressed in S. pombe, immunoblotting, and kinase assays were performed as described [K. Shiozaki and P. Russell, Nature 378, 739 (1995)].
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(1991)
Methods Enzymol.
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, pp. 795
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Moreno, S.1
Klar, A.2
Nurse, P.3
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19
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0029563651
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+. All strains were leu1-32 ura4-D18. All nmt1 promoter constructs were integrated at the leu1-32 locus. Growth media and general methods for S. pombe have been described [S. Moreno, A. Klar, P. Nurse, Methods Enzymol. 194, 795 (1991)]. Unless otherwise indicated, yeast cultures were grown in YES medium at 30°C. YES consists of glucose, yeast extract, and amino acid supplements. HU was used at a concentration of 12 mM. Purification of GST fusion proteins and hexahis-tagged proteins expressed in S. pombe, immunoblotting, and kinase assays were performed as described [K. Shiozaki and P. Russell, Nature 378, 739 (1995)].
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(1995)
Nature
, vol.378
, pp. 739
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Shiozaki, K.1
Russell, P.2
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20
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0027173751
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M. Fernandez-Sarabia, C. McInerny, P. Harris, C. Gordon, P. Fantes, Mol. Gen. Genet. 238, 241 (1993).
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Fernandez-Sarabia, M.1
McInerny, C.2
Harris, P.3
Gordon, C.4
Fantes, P.5
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22
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2642602592
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note
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+ construct (7).
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23
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2642664011
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The in vivo interaction between GST:Cds1 and Wee1 shown in Fig. 20 did not require HU treatment, presumably because GST:Cds1 was overexpressed
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The in vivo interaction between GST:Cds1 and Wee1 shown in Fig. 20 did not require HU treatment, presumably because GST:Cds1 was overexpressed.
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24
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2642598539
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note
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600 of 0.3 in YES medium and then diluted to 13,000 cells/ml in YES media containing HU (12 mM), followed by growth at 30°C for a further 8 hours. Samples (75 μl) were taken at regular intervals, plated onto YES plates, and grown for 4 days at 30°C to determine survival.
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28
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2642636375
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We thank N. Rhind, J.-M. Brondello, C. McGowan, and K. Shiozaki for their advice and the Scripps Cell Cycle Groups for their support and encouragement. Funded by NIH
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We thank N. Rhind, J.-M. Brondello, C. McGowan, and K. Shiozaki for their advice and the Scripps Cell Cycle Groups for their support and encouragement. Funded by NIH.
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