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Volumn 280, Issue 5365, 1998, Pages 909-912

Replication checkpoint enforced by kinases Cds1 and Chk1

Author keywords

[No Author keywords available]

Indexed keywords

HYDROXYUREA; PROTEIN KINASE;

EID: 0032496322     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5365.909     Document Type: Article
Times cited : (282)

References (28)
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    • Y. Sanchez et al., ibid., p. 1497; C.-Y. Peng et al., ibid., p. 1501.
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    • Y. Sanchez et al., ibid., p. 1497; C.-Y. Peng et al., ibid., p. 1501.
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    • note
    • 70 truncation product were replicated with a precise fusion of GST and amino acids 1 to 72 of Wee1.
  • 18
    • 0026025891 scopus 로고
    • +. All strains were leu1-32 ura4-D18. All nmt1 promoter constructs were integrated at the leu1-32 locus. Growth media and general methods for S. pombe have been described [S. Moreno, A. Klar, P. Nurse, Methods Enzymol. 194, 795 (1991)]. Unless otherwise indicated, yeast cultures were grown in YES medium at 30°C. YES consists of glucose, yeast extract, and amino acid supplements. HU was used at a concentration of 12 mM. Purification of GST fusion proteins and hexahis-tagged proteins expressed in S. pombe, immunoblotting, and kinase assays were performed as described [K. Shiozaki and P. Russell, Nature 378, 739 (1995)].
    • (1991) Methods Enzymol. , vol.194 , pp. 795
    • Moreno, S.1    Klar, A.2    Nurse, P.3
  • 19
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    • +. All strains were leu1-32 ura4-D18. All nmt1 promoter constructs were integrated at the leu1-32 locus. Growth media and general methods for S. pombe have been described [S. Moreno, A. Klar, P. Nurse, Methods Enzymol. 194, 795 (1991)]. Unless otherwise indicated, yeast cultures were grown in YES medium at 30°C. YES consists of glucose, yeast extract, and amino acid supplements. HU was used at a concentration of 12 mM. Purification of GST fusion proteins and hexahis-tagged proteins expressed in S. pombe, immunoblotting, and kinase assays were performed as described [K. Shiozaki and P. Russell, Nature 378, 739 (1995)].
    • (1995) Nature , vol.378 , pp. 739
    • Shiozaki, K.1    Russell, P.2
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    • note
    • + construct (7).
  • 23
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    • The in vivo interaction between GST:Cds1 and Wee1 shown in Fig. 20 did not require HU treatment, presumably because GST:Cds1 was overexpressed
    • The in vivo interaction between GST:Cds1 and Wee1 shown in Fig. 20 did not require HU treatment, presumably because GST:Cds1 was overexpressed.
  • 24
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    • note
    • 600 of 0.3 in YES medium and then diluted to 13,000 cells/ml in YES media containing HU (12 mM), followed by growth at 30°C for a further 8 hours. Samples (75 μl) were taken at regular intervals, plated onto YES plates, and grown for 4 days at 30°C to determine survival.
  • 28
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    • We thank N. Rhind, J.-M. Brondello, C. McGowan, and K. Shiozaki for their advice and the Scripps Cell Cycle Groups for their support and encouragement. Funded by NIH
    • We thank N. Rhind, J.-M. Brondello, C. McGowan, and K. Shiozaki for their advice and the Scripps Cell Cycle Groups for their support and encouragement. Funded by NIH.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.