A genome-wide functional assay of signal transduction in living mammalian cells

Nature Biotechnology

Volumn 16, Issue 13, 1998, Pages 1329-1333

  Whitney, Mike ^a   Rockenstein, Edward ^a   Cantin, Greg ^a   Knapp, Tom ^a   Zlokarnik, Gregor ^a   Sanders, Pam ^a   Durick, Kyle ^a   Craig, Frank F ^a   Negulescu, Paul A ^a  

^a Aurora Biosciences Corporation   (United States)

Author keywords

lactamase; Flow cytometry; Gene trap; T cell

Indexed keywords

BETA LACTAMASE;

ANIMAL CELL; ARTICLE; CELL ISOLATION; CELL LINE; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; REPORTER GENE; SIGNAL TRANSDUCTION;

BETA-LACTAMASES; CELL SEPARATION; ENZYME ACTIVATION; FLOW CYTOMETRY; FLUORESCENT DYES; GENES, REPORTER; GENOME; HUMANS; JURKAT CELLS; SIGNAL TRANSDUCTION; TETRADECANOYLPHORBOL ACETATE; THAPSIGARGIN;

ANIMALIA; MAMMALIA;

EID: 0031733589     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/4302     Document Type: Article

References (28)

1. Heller, R.A., Schena, M., Chai, A., Shalon, D., Bedilion, T., Gilmore, J. et al. 1997. Discovery and analysis of inflammatory disease-related genes using cDNA microarrays. Proc. Natl. Acad. Sci. USA 94:2150-2155.
2. Schena, M., Shalon, D., Heller, R., Chai, A., Brown, P.O., and Davis, R.W. et al. 1996. Parallel human genome analysis: microarray-based expression monitoring of 1000 genes. Proc. Natl. Acad. Sci. USA 93:10614-10619.
3. Chee, M., Yang, R., Hubbell, E., Berno, A., Huang, X.C., Stern, D. et al. 1996. Accessing genetic information with high-density arrays. Science 274:610-614.
4. Skarnes, W.C., Auerbach, B.A., and Joyner, A.L. 1992. A gene trap approach in mouse embryonic stem cells: the lacZ reporter is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice. Genes Dev. 6:903-918.
5. Forrester, L.M., Nagy, A., Sam, M., Watt, A., Stevenson, L., Berstein, A. et al. 1996. An induction gene trap screen in embryonic stem cells: identification of genes that respond to retenoic acid in vitro. Proc. Natl. Acad. Sci. USA 93:1677-1682.
6. Zlokarnik, G., Negulescu, P.A., Knapp, T.E., Mere, L., Burres, N., Feng., L. et al. 1998. Quantitation of transcription and clonal selection of single living cells using beta-lactamase as reporter. Science 279:84-88.
7. Wurst, W., Rossant, J., Prideaux, V., Kownacka, M., Joyner, A., Hill, D.P. et al. 1995. A large-scale gene-trap screen for insertional mutation in developmentally regulated genes in mice. Genetics 139:889-899.
8. Gogos, J.A., Lowry, W., and Karayiorgou, M. 1997. Selection for retroviral insertions into regulated genes. J. Virol. 71:1644-1650.
9. Alberola-lla, J., Takaki, S., Kerner, J.D., and Perlmutter, R.M. 1997. Differential signaling by lymphocyte antigen receptors. Annu. Rev. Immunol. 15:125-154.
10. Truneh, A., Albert, F., Goldstein, P., and Schmitt-Verhulst, A.M. 1985. Early steps of lymphocyte activation bypassed by synergy between calcium ionophores and phorbol ester. Nature 313:318-320.
11. 2(+)-ATPase. Proc. Natl. Acad. Sci. USA 87:2466-2470.
12. Takemura, H., Hughes, A.R., Thastrup, O., and Putney, J.W. 1989. Activation of calcium entry by the tumor promoter thapsigargin in parotid acinar cells. J. Biol. Chem. 264:12266-12271.
13. Rosenstreich, D.L. and Mizel, S.B. 1979. Signal requirements for T lymphocyte activation: replacement of macrophage function with phorbol myristic acetate. J. Immunol. 123:1749-1755.
14. Clipstone, N.A. and Crabtree, G.R. 1992. Identification of calcineurin as a key signaling enzyme in T-lymphocyte activation. Nature 357:695-697.
15. Elliot, J.F., Lin, Y., Mizel, S.B., Bleackley, R.C., Harnish, D.G., and Paetkau, V. 1984. Induction of interleukin 2 messenger RNA inhibited by cyclosporin A. Science 226:1439-1443.
16. Negulescu, P.A., Shastri, N., and Calahan, M.D. 1994.Intracellular calcium dependence of gene expression in single T lymphocytes. Proc. Natl. Acad. Sci. USA 91:2873-2877.
17. Fiering, S. and Al., E. 1990. Single cell assay of a transcription factor reveals a threshold in transcription activated by signals emanating from the T-cell antigen receptor. Genes Dev.4:1823-1830.
18. Frohman, M.A., Dush, M.K., and Martin, G.R. 1988. Rapid production of full length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85:8998-9002.
19. Nagasawa, M., Melamed, I., Kupfer, A., Gelfand, E.W., and Lucas, J.J. 1997. Rapid nuclear translocation and increased activity of cyclin-dependent kinase 6 after T-cell activation. J. Immunol. 158:5146-5154.
20. Mages, H.W., Stamminger, T., Rilke, O., Bravo, R., and Kroczek, R.A. 1993. Expression of PILOT, a putative transcription factor, requires two signals and is cyclosporin A sensitive in T-cells. Int. Immunol. 5:63-70.
21. Patwardhan, S., Gashler, A., Siegel, M.G., Chang, L.C., Joseph, L.J., Shows, T.B. et al. 1991. EGR3, a novel member of the Egr family of genes encoding immediate-early transcription factors. Oncogene 6:917-928.
22. Korchak, H.M., Kane, L.H., and Rossi, M.W. 1994. Long chain acyl coenzyme A and signaling in neutrophils. An inhibitor of acyl coenzyme A synthetase, triacsin C, inhibits superoxide anion generation and degranulation of human neutrophils. J. Biol. Chem. 269:30281-30287.
23. Faergeman, M.J. and Knudsen, J. 1997. Role of long-chain fatty acyl-CoA esters in the regulation of metabolism and in cell signalling. Biochem. J. 323:1-12.
24. Townley, D.J., Avery, B.J., Rosen, B., and Skarnes, W.C. 1997.Rapid sequence analysis of gene trap integrations to generate a resourse of insertional mutations in mice. Genome Res.7:293-298.
25. Reddy, S., Rayburn, H., von Melcher, H., and Ruley, H.E. 1992. Fluorescence-activated sorting of titopotent embryonic stem cells expressing developmentally regulated lacZ fusion genes. Proc. Natl. Acad. Sci. USA 89:6721-6725.
26. Nolan, G.P., Fiering, S., Nicolas, J.F., and Herzenberg, L.A. 1988. Flourescence-activated cell analysis and sorting of viable mammalian cells based on β-D-galactosidase activity after transduction of E. coli lacZ. Proc. Natl. Acad. Sci. USA 85:2603-2607.
27. Chowdury, H., Bonaldo, P., Torres, M., Stoykova, A., and Gruss, P. 1997. Evidence for the satochastic integration of gene trap vectors into the mouse germline. Nucleic Acids Res. q25:1531-1536.
28. Miller, S.A., Dykes, D.D., and Polesky, H.F. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 16:1215.