Engineering protein kinases with distinct nucleotide specificities and inhibitor sensitivities by mutation of a single amino acid

Chemistry and Biology

Volumn 5, Issue 7, 1998, Pages

  Cohen, Philip ^a   Goedert, Michel ^b  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

^b MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0032127543     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(98)90068-0     Document Type: Review

References (14)

1. Shah, K., Liu, Y., Deirmengian, C. & Shokat, K.M. (1997). Engineering unnatural nucleotide specificity for Rous sarcoma virus tyrosine kinase to uniquely label its direct substrates. Proc. Natl Acad. Sci. USA 94, 3565-3570.
2. Liu, Y., Shah, K., Yang, F., Witucki, L & Shokat, K.M. (1998). Engineering Src family protein kinases with unnatural nucleotide specificity. Chem. Biol. 5, 91-101.
3. Bishop, A.C., Shah, K., Liu, Y, Witucki, L., Kung, C.-Y. & Shokat, K.M. (1998). Design of allele-specific inhibitors to probe protein kinase signaling. Curr. Biol. 8, 257-266.
4. Sicheri, F., Moarefi, I. & Kuriyan, J. (1997). Crystal structure of the Src-family tyrosine kinase Hck. Nature 385, 602-609.
5. Lee, J.C., et al., & Young, P.R. (1994). A protein kinase involved in the regulation of inflammatory cytokine biosynthesis. Nature 372, 739-746.
6. Badger, A.M., Bradbeer, J.N., Votta, B., Lee, J.C.. Adams, J.L & Griswold, D.E. (1996). Pharmacological profile of SB 203580, a selective inhibitor of cytokine suppressive binding protein/p38 kinase, in animal models of arthritis, bone resorption, endotoxin shock and immune function. J. Pharmacol. Exp. Ther. 279, 1453-1461.
7. Cuenda, A., et al., & Lee, J.C. (1995). SB 203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin-1. FEBS Lett. 364, 229-231.
8. Goedert, M., Cuenda, A., Craxton, M., Jakes, R. & Cohen, P. (1997). Activation of the novel stress-activated protein kinase SAPK4 by cytokines and cellular stresses is mediated by SKK3 (MKK6); comparison of its substrate specificity with that of other SAP kinases. EMBO J. 16, 3563-3571.
9. Cohen, P. (1997) The search for physiological substrates of MAP and SAP kinases in mammalian cells. Trends Cell Biol. 7, 353-361.
10. Young. P.R., et al., & Lee, J.C. (1997). Pyridinyl imidazole inhibitors of p38 mitogen-activated protein kinase bind in the ATP site. J. Biol. Chem. 272, 12116-12121.
11. Tong, L., et al., & Pargellis, C.A. (1997). A highly specific inhibitor of human p38 MAP kinase binds in the ATP pocket. Nat. Struct. Biol. 4, 311-316.
12. Wilson, K.P., et al., & Su, M.S.S. (1997). The structural basis for the specificity of pyridinylimidazole inhibitors of p38 MAP kinase. Chem. Biol. 4, 423-431.
13. Eyers, P.A., Craxton, M., Morrice, N., Cohen. P. & Goedert, M. (1998). Conversion of SB 203580-insensitive MAP kinase family members to drug-sensitive forms by a single amino acid substitution. Chem. Biol. 5, 321-328.
14. Gum, R.J., et al., & Young, P.R. (1998). Acquisition of sensitivity of stress-activated protein kinases to the p38 inhibitor, SB 203580, by alteration of one or more amino acids within the ATP binding pocket. J. Biol. Chem., in press.