Failure of hairpin-ended and nickel DNA to activate DNA-dependent protein kinase: Implications for V(D)J recombination

Molecular and Cellular Biology

Volumn 18, Issue 11, 1998, Pages 6853-6858

  Smider, Vaughn ^a   Rathmell, W Kimryn ^a   Brown, Greg ^b   Lewis, Susanna ^b   Chu, Gilbert ^a  

^a STANFORD UNIVERSITY MEDICAL CENTER   (United States)

^b HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN KINASE;

ARTICLE; CATALYSIS; DNA NICK TRANSLATION; DNA RECOMBINATION; ENZYME ACTIVATION; ENZYME ACTIVITY; GENETIC CODE; IONIZING RADIATION; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; PROTEIN ANALYSIS; SIGNAL TRANSDUCTION;

EID: 0031741234     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/mcb.18.11.6853     Document Type: Article

References (34)

1. Agrawal, A., and D. Schatz. 1997. RAG1 and RAG2 form a stable postcleavage synaptic complex with DNA containing signal ends in V(D)J recombination. Cell 89:43-53.
2. Biedermann, K., J. Sun, A. Giaccia, L. Tosto, and J. M. Brown. 1991. Scid mutation in mice confers hypersensitivity to ionizing radiation and a deficiency in DNA double-strand break repair. Proc. Natl. Acad. Sci. USA 88:1394-1397.
3. Blier, P., A. Griffith, J. Craft, and J. Hardin. 1993. Binding of Ku protein to DNA. Measurement of affinity for ends and demonstration of binding to nicks. J. Biol. Chem. 268:7594-7601.
4. Blunt, T., N. Finnie, G. Taccioli, G. Smith, J. Demengeot, T. Gottlieb, R. Mizuta, A. Varghese, F. Alt, P. Jeggo, and S. Jackson. 1995 Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation. Cell 80:813-823.
5. Blunt, T., D. Gell, M. Fox, G. Taccioli, S. Jackson, A. Lehman, and P. Jeggo. 1996. Identification of a nonsense mutation in the carboxy-terminal region of DNA-dependent protein kinase catalytic subunit in the scid mouse. Proc. Natl. Acad. Sci. USA 93:10285-10290.
6. Chu, G. 1997. Double-strand break repair. J. Biol. Chem. 272:24097-24100.
7. Danska, J., D. Holland, S. Mariathasan, K. Williams, and C. Guidos. 1996. Biochemical and genetic defects in the DNA-dependent protein kinase in murine scid lymphocytes. Mol. Cell. Biol. 16:5507-5517.
8. Errami, A., V. Smider, W. K. Rathmell, D. He, E. A. Hendrickson, M. Zdzienicka, and G. Chu. 1996. Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cells. Mol. Cell. Biol. 16:1519-1526.
9. Falzon, M., J. W. Fewell, and E. L. Kuff. 1993. EBP-80, a transcription factor closely resembling the human autoantigen Ku, recognizes single-to double-strand transitions in DNA. J. Biol. Chem. 268:10546-10552.
10. Fulop, G., and R. Phillips. 1990. The scid mutation in mice causes a general defect in DNA repair. Nature 347:479-482.
11. Gottlieb, T., and S. Jackson. 1993. The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell 72:131-142.
12. Hammarsten, O., and G. Chu. 1998. DNA-dependent protein kinase: DNA binding and activation in the absence of Ku. Proc. Natl. Acad. Sci. USA 95:525-530.
13. Hendrickson, E., X. Q. Qin, E. Bump, D. Schatz, M. Oettinger, and D. Weaver. 1991. A link between double-strand break related repair and V(D)J recombination: the scid mutation. Proc. Natl. Acad. Sci. USA 88:4061-4065.
14. Kirchgessner, C., C. Patil, J. Evans, C. Cuomo, L. Fried, T. Carter, M. Oettinger, and J. M. Brown. 1995. DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science 267:1178-1185.
15. Lees-Miller, S., K. Sakaguchi, S. Ullrich, E. Appela, and C. Anderson. 1992. Human DNA-activated protein kinase phosphorylates serines 15 and 37 in the amino-terminal transactivation domain of human p53. Mol. Cell. Biol. 12:5041-5049.
16. Leu, T., Q. Eastman, and D. Schatz. 1997. Coding joint formation in a cell-free V(D)J recombination system. Immunity 7:303-314.
17. Lewis, S. M. 1994. P nucleotide insertions, and the resolution of hairpin DNA structures in mammalian cells. Proc. Natl. Acad. Sci. USA 91:1332-1336.
18. Lieber, M. R., J. E. Hesse, S. Lewis, G. C. Bosma, N. Rosenberg, K. Mizuuchi, M. J. Bosma, and M. Gellert. 1988. The defect in murine severe combined immune deficiency: joining of signal sequences but not coding segments in V(D)J recombination. Cell 55:7-16.
19. McBlane, F., D. van Gent, D. Ramsden, C. Romeo, C. Cuomo, M. Gellert, and M. Oettinger. 1995. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps. Cell 83:387-395.
20. Meier, J., and S. Lewis. 1993. P nucleotides in V(D)J recombination: a fine-structure analysis. Mol. Cell. Biol. 13:1078-1092.
21. Morozov, V., M. Falzon, C. Anderson, and E. Kuff. 1994. DNA-dependent protein kinase is activated by nicks and larger single-stranded gaps. J. Biol. Chem. 269:16684-16688.
22. Paillard, S., and F. Strauss. 1991. Analysis of the mechanism of interaction of simian Ku protein with DNA. Nucleic Acids Res. 19:5619-5624.
23. Pergola, F., M. Z. Zdzienicka, and M. R. Lieber. 1993. V(D)J recombination in mammalian cell mutants defective in DNA double-strand break repair. Mol. Cell. Biol. 13:3464-3471.
24. Ramsden, D., T. Paull, and M. Gellert. 1997. Cell-free V(D)J recombination. Nature 388:488-491.
25. Roth, D., J. Menetski, P. Nakajima, M. Bosma, and M. Gellert. 1992. V(D)J recombination: broken DNA molecules with covalently sealed (hairpin) coding ends in scid mouse thymocytes. Cell 70:983-991.
26. Shore, D., J. Langowski, and R. Baldwin. 1981. DNA flexibility studied by covalent closure of short DNA fragments into circles. Proc. Natl. Acad. Sci. USA 78:4833-4837.
27. Smider, V., and G. Chu. 1997. The end-joining reaction in V(D)J recombination. Semin. Immun. 9:189-197.
28. Smider, V., W. K. Rathmell, M. Lieber, and G. Chu. 1994. Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science 266:288-291.
29. Taccioli, G., T. Gottlieb, T. Blunt, A. Priestly, J. Demengeot, R. Mizuta, A. Lehmann, F. Alt, S. Jackson, and P. Jeggo. 1994. Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination. Science 265:1442-1445.
30. van Gent, D., J. F. McBlane, D. Ramsden, M. Sadofsky, J. Hesse, and M. Gellert. 1995. Initiation of V(D)J recombination in a cell-free system. Cell 81:925-934.
31. van Gent, D., D. Ramsden, and M. Gellert. 1996. The RAG1 and RAG2 proteins establish the 12/23 rule in V(D)J recombination. Cell 85:107-113.
32. Weinfeld, M., M. Chaudhry, D. D'Amours, J. Pelletier, G. Poirer, L. Povirk, and S. Lees-Miller. 1998. Interaction of DNA-dependent protein kinase and poly(ADP-ribose) polymerase with radiation-induced DNA strand breaks. Radiat. Res. 148:22-28.
33. Yaneva, M., T. Kowalewski, and M. Lieber. 1997. Interaction of DNA-dependent protein kinase with DNA and with Ku: biochemical and atomic-force microscopy studies. EMBO J. 16:5098-5112.
34. Zhu, C., M. Bogue, D. S. Lim, P. Hasty, and D. Roth. 1996. Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates. Cell 86:379-389.